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Summary of Contents for Thermo Scientific Sorvall BIOS A
- Page 1 Thermo Scientific Sorvall BIOS A Centrifuge and Heavy Duty Centrifuge Instruction Manual 50154616-c • 03 / 2021 Visit us online to register your warranty: thermofisher.com/labwarranty...
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Page 2: Table Of Contents
Items Supplied . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi Intended Use for Sorvall BIOS A and Sorvall BIOS A Heavy Duty . . . . . . . . . . . vii Associated Rotors and Accessories.................. - Page 3 Thermo Scientific Sorvall BIOS A Centrifuges 3 . Install a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .III–2 3.
- Page 4 Thermo Scientific Sorvall BIOS A Centrifuges 2 . Ice Formation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . V-2 3 .
- Page 5 Table I–1: Overview of necessary tools for setting up ............I–3 Table V–1: Error messages ....................V-3 Table A–1: Technical Data Thermo Scientific Sorvall BIOS A and Sorvall BIOS A Heavy Duty ..A–1 Table A–2: Directives, Standards and Guidelines Sorvall BIOS A and Sorvall BIOS A Heavy Duty .........................A–2...
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Page 6: Preface
Thermo Scientific Sorvall BIOS A, 380, 400, 415 V ±10%, 50/60 Hz, 3-phase 75007700 Thermo Scientific Sorvall BIOS A Heavy Duty, 200, 208, 220, 230, 240 V ±10%, 60 Hz, Single phase Japan only: 200 V ±10%, 50 Hz Power Supply Cable... -
Page 7: Intended Use For Sorvall Bios A And Sorvall Bios A Heavy Duty
Associated Rotors and Accessories The Thermo Scientific ™ Sorvall ™ BIOS A and Thermo Scientific Sorvall BIOS A Heavy Duty centrifuge can be operated with the Thermo Scientific ™ Fiberlite ™ F6-10x1000 LEX rotor. For more details on accessories refer to “Rotors”... -
Page 8: Preparation
Thermo Scientific Sorvall BIOS A Centrifuges Preface The mains plug must be freely accessible at all times. ƒ ƒ To shut down the centrifuge: Press the STOP key. Turn off the centrifuge at the main switch. Pull out the power supply plug. -
Page 9: Operating
Thermo Scientific Sorvall BIOS A Centrifuges Preface Operating ƒ Never use the centrifuge if parts of its casing is damaged or missing. ƒ Do not move the centrifuge while it is running. ƒ Do not lean on the centrifuge. ƒ... -
Page 10: Symbols Used On The Centrifuge
Thermo Scientific Sorvall BIOS A Centrifuges Preface Symbols used on the Centrifuge This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. CAUTION means that material damage could occur. -
Page 11: Symbols Used In The Manual
Thermo Scientific Sorvall BIOS A Centrifuges Preface Symbols used in the Manual This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. CAUTION means that material damage could occur. -
Page 12: Transport And Set Up
Thermo Scientific Sorvall BIOS A Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. Transport and Set Up CA UT I O N Make sure that the centrifuge and the packaging is not damaged. Speak to the shipping company and Thermo Fisher Scientific immediately if damage is found. -
Page 13: Transporting
Thermo Scientific Sorvall BIOS A Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. Figure I–1: Centrifuge clearance, example with barcode scanner 2. Transporting WARN I N G Never stand before a moving centrifuge to stop it. -
Page 14: Setting Up
Thermo Scientific Sorvall BIOS A Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. 3. Setting Up CAU T I ON Do not push at the touchscreen. Due to the weight of the centrifuge it must be controlled by two or more people when rolling of the pallet. Do not stand in front of the centrifuge when rolling it down the ramp. -
Page 15: Level The Centrifuge
Thermo Scientific Sorvall BIOS A Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. Level the Centrifuge CAU T I ON If you do not level the centrifuge, the centrifuge can crash because of imbalance. -
Page 16: Mains Connection
Thermo Scientific Sorvall BIOS A Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. 4. Mains Connection N OT I C E Connect the centrifuge into grounded electrical sockets only. Figure I–3: Mains connection on the backside of the centrifuge Turn off the power supply switch located on the right side (pull the switch handle towards the front of the centrifuge). -
Page 17: Shipping
Thermo Scientific Sorvall BIOS A Centrifuges Transport and Set Up THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS. 6. Shipping WA R N I N G Before shipping the centrifuge and accessories you must clean and if necessary, disinfect or decontaminate the full system. -
Page 18: Thermo Scientific
Thermo Scientific Sorvall BIOS A Centrifuges Thermo Scientific Centri-Touch User Interface Thermo Scientific Centri-Touch User Interface Actual status information ➀ Set values ➁ Settings and navigation ➂ II–1... -
Page 19: Status
Thermo Scientific Sorvall BIOS A Centrifuges Thermo ScientificCentri-Touch User Interface Status The upper part of the touchscreen displays the centrifuge status. The remaining time is indicated while centrifugation is in progress. The progress bar shows the actual phase of the centrifugation. -
Page 20: Run Parameters
Thermo Scientific Sorvall BIOS A Centrifuges Thermo ScientificCentri-Touch User Interface Run Parameters The set points for centrifugation can be set in the parameterization window. Clicking one of these buttons will open a new window in which you can input the appropriate set point value. -
Page 21: Lighthouse Mode
Thermo Scientific Sorvall BIOS A Centrifuges Thermo ScientificCentri-Touch User Interface Lighthouse Mode If no entry is made via the main screen in 30 seconds, the unit changes to lighthouse mode. In this mode, the status, current values and progress display are displayed larger. -
Page 22: Operation
Thermo Scientific Sorvall BIOS A Centrifuges Operation I I I Operation For detailed instructions and information refer to the separate manual for the Thermo Scientific ™ Centri-Touch ™ User Interface. 1. Switch on the Centrifuge Push the switch at the right side. -
Page 23: Install A Rotor
Thermo Scientific Sorvall BIOS A Centrifuges Operation 3. Install a Rotor WARN I N G If you have not installed the rotor correctly, do not operate it. Make sure that the rotor is not damaged. Do not use damaged rotors. -
Page 24: Figure Iii-2: Rotor Installation
Thermo Scientific Sorvall BIOS A Centrifuges Operation Figure III–2: Rotor installation Use the rotor locking tool (097-1406) clockwise to tighten the rotor on the drive shaft. Tighten the rotor locking pin until you reach a firm stop. CAUTION Use only the supplied rotor locking tool (097-1406) to avoid an overload on the fixation parts. Using any other tool, like a screwdriver, or over-tightening of the rotor locking pin can lead to an improper installation of the rotor possibly causing severe damage to the rotor and the centrifuge. -
Page 25: Figure Iii-4: Correct Loading
Thermo Scientific Sorvall BIOS A Centrifuges Operation Correct Loading ✓ Figure III–4: Correct Loading Incorrect Loading ✕ Figure III–5: Incorrect Loading III–4... -
Page 26: Maximum Loading
Thermo Scientific Sorvall BIOS A Centrifuges Operation Put the rotor lid on the rotor body. NOTICE If a centrifugation run is done without the rotor lid in place, the centrifuge will not be able to process the desired application as intended. -
Page 27: Use Of Tubes And Consumables
Thermo Scientific Sorvall BIOS A Centrifuges Operation Use of Tubes and Consumables Care should be taken to ensure that the tubes and bottles used in the centrifuge are: rated to or above the selected RCF to be spun at, ƒ... -
Page 28: Runtime Preselection
Thermo Scientific Sorvall BIOS A Centrifuges Operation Explanation of RCF-Value The relative centrifugal force (RCF) is given as a multiple of the force of gravity (g). It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device. -
Page 29: Programs
Thermo Scientific Sorvall BIOS A Centrifuges Operation 7. Programs For detailed instructions and information refer to the according manual for the Thermo Scientific Centri-Touch User Interface. The instructions stated here are just an extract of how to work with programs. Press on the touchscreen. -
Page 30: Centrifugation
Thermo Scientific Sorvall BIOS A Centrifuges Operation 8. Centrifugation WA R N I N G Do not operate the centrifuge on its casters. 8. 1. Start Press the Start key on the touchscreen. The centrifuge accelerates to the preset speed with the time display active. After 30 seconds the touchscreen switches to the lighthouse mode. -
Page 31: Using The Drain Box
Thermo Scientific Sorvall BIOS A Centrifuges Operation 8. 3. Using the Drain Box You can use the drain box (75007730) to collect water from of the centrifugation chamber. Figure III–8: Drain box (75007730) Install the drain box. Figure III–9: Installing the drain box (75007730) Remove the plug from the bottom of the centrifugation chamber. -
Page 32: Remove A Rotor
Thermo Scientific Sorvall BIOS A Centrifuges Operation 9. Remove a Rotor WA R N I N G Risk of cuts through fast rotating rotor lid. Do not open the centrifuge door while the rotor assembly spins. Only when the rotor assembly has stopped, use the mechanical emergency centrifuge door release. -
Page 33: Switch Off The Centrifuge
Thermo Scientific Sorvall BIOS A Centrifuges Operation Figure III–12: Disengage the rotor locking pin from drive shaft Grab the rotor without the rotor lid on the inner groove with your hands. Remove the rotor body from the drive shaft. NOTICE Two persons are necessary for this procedure because of the weight of a rotor. -
Page 34: Maintenance And Care
Thermo Scientific Sorvall BIOS A Centrifuges Maintenance and Care Maintenance and Care 1. Cleaning Intervals For the sake of personal, environmental and material protection, you must clean and if necessary disinfect the centrifuge and its accessories on a regular basis. -
Page 35: Rotor And Accessories Inspection
Press Settings (gears symbol), then Runs and then Rotor Log to see the information. Besides the values of the rotor an information about the number of cycles completed by this rotor type in this centrifuge is shown. For detailed instructions and information refer to the separate manual for the Thermo Scientific Centri-Touch User Interface. IV–2... -
Page 36: Cleaning
Thermo Scientific Sorvall BIOS A Centrifuges Maintenance and Care 3. Cleaning CAU T I ON Before using any cleaning methods, users should check with the manufacturer of the cleaning agents that the proposed method will not damage the equipment. CAU T I ON Drive and door lock can be damaged by entering liquids. -
Page 37: Filter Mat
Thermo Scientific Sorvall BIOS A Centrifuges Maintenance and Care 3. 2. Filter Mat CAU T I ON Do not touch the edges of the housing with your hands when the grid is removed. Cutting damage can occur if you touch the edges due to their sharpness. -
Page 38: Disinfection
Thermo Scientific Sorvall BIOS A Centrifuges Maintenance and Care 4. Disinfection WA R N I N G Do not touch infected parts. Hazardous infection is possible when touching the contaminated rotor and centrifuge parts. Infectious material can get into the centrifuge when a tube breaks or as a result of spills. -
Page 39: Autoclaving
Thermo Scientific Sorvall BIOS A Centrifuges Maintenance and Care 6. Autoclaving CA UT I O N Never exceed the permitted temperature and duration when autoclaving. N OT I C E No chemical additives are permitted in the steam. The rotor body is too big to be autoclaved. If not stated otherwise all other parts can be autoclaved at 121 °C for 20 min. -
Page 40: Shipping And Disposal
Thermo Scientific Sorvall BIOS A Centrifuges Maintenance and Care 8. Shipping and Disposal WA R N I N G When removing the centrifuge and accessories from use for disposal you have to clean and if necessary disinfect or decontaminate the entire system. In doubt contact the Thermo Fisher Scientific customer service. -
Page 41: Troubleshooting
Thermo Scientific Sorvall BIOS A Centrifuges Troubleshooting Troubleshooting 1. Mechanical Emergency Door Release WA R N I N G Serious injuries can occur, if you touch a spinning rotor with your hands or tools. A rotor can still be spinning after a power failure occurs. -
Page 42: Ice Formation
Thermo Scientific Sorvall BIOS A Centrifuges Troubleshooting Pull out the power supply plug. On both sides of the housing are two white plastic plugs. Get these two white plastic plugs out of both sides of the housing. Pull the release cords on both sides to trigger the mechanical centrifuge door release. The centrifuge door needs to be lifted manually. -
Page 43: Troubleshooting By Guide
Thermo Scientific Sorvall BIOS A Centrifuges Troubleshooting 3. Troubleshooting by Guide CAU T I ON If problems occur other than those listed in this table, service technician must be contacted. If an error occurs, follow the instructions on the touchscreen. Error messages have a red colored background. -
Page 44: Information For The Customer Service
Thermo Scientific Sorvall BIOS A Centrifuges Troubleshooting 4. Information for the Customer Service If you need to contact a service technician, please provide the order no. and the serial no. of your centrifuge. This information can be found on the back near the inlet for the power supply cable. -
Page 45: Technical Specifications
380, 400, 415 V, 50/60 Hz 466 kg (1 027 lb) 1 m in front of the instrument at 1.6 m height. Without rotor. Table A–1: Technical Data Thermo Scientific Sorvall BIOS A and Sorvall BIOS A Heavy Duty A–1... -
Page 46: Directives, Standards And Guidelines
200 V, 50 / 60 Hz IEC 61326-1 Class B China EN ISO 14971 3-Phase 380 V, 50 Hz EN ISO 9001 Table A–2: Directives, Standards and Guidelines Sorvall BIOS A and Sorvall BIOS A Heavy Duty 2. Refrigerants Article No. Centrifuge Refrigerant Quantity Pressure... -
Page 47: Mains Supply
Thermo Scientific Sorvall BIOS A Centrifuges Technical Specifications 3. Mains Supply The following data is to be taken into consideration, when selecting the mains connection socket. Power Building Equipment Mains Voltage Frequency Rated Current Consumption Fuse Fuse 200 V 50 Hz 32.0 A... -
Page 48: Rotors
Thermo Scientific Sorvall BIOS A Centrifuges Rotors Rotors Items Supplied for Rotor Article No. Item Quantity 096-101001 Thermo Scientific Fiberlite F6-10x1000 LEX Rotor 099-101001 Lid Assembly 020-101001 Rotor Care Kit 097-1403 1L Closure Tool 097-1406 10x1000 LEX Polars 5 mm tie-down T-Wrench... -
Page 49: Fiberlite F6-10X1000 Lex Rotor
Thermo Scientific Sorvall BIOS A Centrifuges Rotors Fiberlite F6-10x1000 LEX Rotor Technical Data Sorvall BIOS A / Sorvall BIOS A Heavy Duty Centrifuge Voltage 200, 208, 220, 230, 240 V 380, 400, 415 V Rotor Capacity 10 x 1 000 ml... - Page 50 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–1...
- Page 51 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–2...
- Page 52 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–3...
- Page 53 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–4...
- Page 54 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–5...
- Page 55 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–6...
- Page 56 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–7...
- Page 57 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–8...
- Page 58 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–9...
- Page 59 Thermo Scientific Sorvall BIOS A Centrifuges Chemical Compatibility Chart C–10...
- Page 60 Thermo Scientific Sorvall BIOS A Centrifuges Index Maintenance and Care IV–1 Maximum Loading III–5 Mechanical Emergency Door Release Metal Parts IV–2 Acceleration / Deceleration Profiles III–6 Open the Centrifuge Door III–1 Accessories A–3 Operating viii Aerosol-tight Rotors III–6 Operation III–1 Autoclaving IV–6...
- Page 61 Thermo Scientific Sorvall BIOS A Thermo Scientific Sorvall BIOS A Heavy Duty Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/centrifuge © 2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated.
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