Solvent Information - Agilent Technologies 1200 Series User Manual

Solvent Information

Flow Cell
To protect optimal functionality of your flow-cell:
• Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and
• If the flow cell is transported while temperatures are below 5 degree C, it
• Aqueous solvents in the flow cell can built up algae. Therefore do not leave
Use of Solvents
Observe the following recommendations on the use of solvents.
• Brown glass ware can avoid growth of algae.
• Small particles can permanently block capillaries and valves. Therefore
• Avoid the use of the following steel-corrosive solvents:
1200 Series TCC User Manual
thus impair the optical properties of the flow cell.
must be assured that the cell is filled with alcohol.
aqueous solvents sitting in the flow cell. Add a small % of organic solvents
(e.g. acetonitrile or methanol ~5%).
always filter solvents through 0.4 µm filters.
• Solutions of alkali halides and their respective acids (for example,
lithium iodide, potassium chloride, and so on),
• High concentrations of inorganic acids like sulfuric acid and nitric acid,
especially at higher temperatures (if your chromatography method
allows, replace by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel),
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl + O —> 2COCl
3 2
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol,
+ 2HCl
2
Appendix

Solvent Information

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