Solvent Information - Agilent Technologies 1200 Series User Manual

A Appendix

Solvent Information

Observe the following recommendations on the use of solvents.
Flow Cell
Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus
impair the optical properties of the flow cell.
Prevent any crystallization of buffer solutions. This will lead into a
blockage/damage of the flow cell.
If the flow cell is transported while temperatures are below 5 degree C, it must
be assured that the cell is filled with alcohol.
Aqueous solvents in the flow cell can built up algae. Therefore do not leave
aqueous solvents sitting in the flow cell. Add small % of organic solvents (e.g.
Acetonitrile or Methanol ~5%).
Solvents
Brown glass ware can avoid growth of algae.
Always filter solvents through 0.4 µm filters, small particles can permanently
block the capillaries. Avoid the use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium
• High concentrations of inorganic acids like nitric acid, sulfuric acid
• Halogenated solvents or mixtures which form radicals and/or acids, for
102
iodide, potassium chloride, and so on).
especially at higher temperatures (replace, if your chromatography method
allows, by phosphoric acid or phosphate buffer which are less corrosive
against stainless steel).
example:
→
2CHCl + O 2COCl
3 2
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
+ 2HCl
2
1200 Series TCC User Manual