Insert Membranes In A Bottle - Thermo Scientific Shake n Stack 6240 Operating And Maintenance Manual

Section 4
Methodology of Hybridisation
Insert Membranes in a
4-2 Shake 'n Stack
1. Assemble these items:
Bottle
• Shallow tray containing 2 x SSPE (SSC).
• Mesh.
• Membrane.
Roll up mesh and membrane (Figure 4-1).
2. Place 0.3-0.5 fl. oz. (10-15 ml) of SSPE (SSC) into a bottle and then
insert the roll centrally (Figure 4-2).
Figure 4-1. Roll Mesh and Membrane
3. Secure cap and, holding bottle horizontally, roll to catch the trailing
edge of the mesh; continue rolling in the same direction until coil of
mesh and membrane is well positioned.
Figure 4-3. Rolling Direction of Bottle
4. Pour out SSPE (SSC) and replace with pre-warmed pre-hybridisation
buffer.
5. Place the bottle in the oven so that it rotates in the oven in the same
direction, as it was unrolled in Step 3.
Figure 4-2. Insert Roll
Figure 4-4. Bottle in Oven Rotation
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