INTRODUCTION............................................................................. 1
Dot/Slot Blotting............................................................................... 3
Plaque Blotting ................................................................................ 5
Southern Blotting............................................................................. 5
Capillary Blotting ............................................................................. 6
Figure 2.1: Capillary Blot Assembly ............................................ 7
Northern Blotting........................................................................................8
Equipment ....................................................................................... 12
Figure 3: Inserting Membranes in a Bottle .................................. 14
Prehybridisation............................................................................... 15
Hybridisation.................................................................................... 15
Washing .......................................................................................... 16
RNA Probes .................................................................................... 19
DNA Hybridisation ........................................................................... 21
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© Thermo Scientific, May 2003. Issue 7
HYBRIDISATION GUIDE
Contents
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