Chapter 4 Daily Operation; Things To Do Before Operating The Tsq System - Thermo Scientific TSQ Series Hardware Manual

Daily Operation

Things to Do Before Operating the TSQ System

Thermo Scientific
To ensure the proper operation of your system, Thermo Fisher Scientific recommends daily
performance of these checks and cleaning procedures for the TSQ system.
Contents
• Things to Do Before Operating the TSQ System
• Things to Do After Operating the TSQ System
Note You do not need to tune and calibrate the mass spectrometer as part of your daily
routine.
Tune parameters are instrument parameters that affect the intensity of the ion signal.
Calibration parameters are instrument parameters that affect the mass accuracy and
resolution of the mass spectrum. You must tune and calibrate the mass spectrometer (that
is, optimize the tune and calibration parameters) perhaps once a quarter. For procedures
on how to tune and calibrate your mass spectrometer, refer to "Automatic Tuning and
Calibrating in the ESI/MS Mode" in the TSQ Series Getting Started Guide.
You must optimize the tune parameters (or change the Tune Method) whenever you
change the type of experiment. For procedures on how to optimize the tune parameters
for your ESI or APCI experiment, refer to "Optimizing the Mass Spectrometer with
Your Compound in ESI/MS/MS Mode" or "Optimizing the Mass Spectrometer
with Your Compound in APCI/MS/MS Mode" in the TSQ Series Getting Started Guide.
Every day before starting analyses, verify that the instrument is ready for operation by doing
the following:
• Check the argon and nitrogen supplies.
• Check the ESI fused-silica sample tube for elongation.
• Check the system vacuum levels.
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