4. At this step, it is important to refer to the manual provided for the LD1255R
(http://www.thorlabs.com/Thorcat/0000/0009-D01.pdf). Failure to follow the instructions there
may damage the LED light source.
5. Make sure you have the correct power supply line voltage for the LD1255R, i.e. PS-12DC-US
for 110V (or PS-12DC-EU for 230V).
6. Turn the Current Control Potentiometer (see LD1255R manual) a full 12 turns counter clockwise
to ensure the LED current is at a minimum. As a quick guide, the LD1255-CAB power cable
connects to the power supply at one end and to pins 1, 2 and 3 on the LD1255R (please see
LD1255R manual). The power cable from the LED light source connects to pins 6 and 7 on the
LD1255R with correct polarity, i.e. the side of the connector on the cable which is marked with a
„1‟ will connect to pin 6 (please see LD1255R manual).
7. Now gradually turn the Current Control Potentiometer and the LED should light up after a few
turns. Otherwise, you may have to check the polarity of the light source connector on the driver
board.
6.9 Thorlabs Optical Trapping Test Accessories
Thorlabs provides a set of accessories that allow the user to get started using the system and verify
proper operation. This can be ordered as the item OTKBTK. This set includes the following items:
1. A sample of non-drying immersion oil for microscopy, Cargille Type B, Cat. No. 16484.
2. One plastic slide, with build in channel with height of 400 microns and volume of 100 micro
liters. The bottom matches coverslip number 1.5.
3. A mini pipette with a 50 micro liter volume.
4. Some
non-functionalized fused silica beads in deionized water, 1 micron in diameter, Bangs
Laboratories, Inc.,
19590-D04-V15; Apr 12, 2012
catalog item:
SS04N/9857. Recommended storage temperature is 2 - 8
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Optical Trap Kit
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C.
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