List Of Optical Performances - Olympus CX33 Instructions Manual

Biological microscope
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8

List of optical performances

The following table shows the optical
performances when combining eyepieces and
objectives.
The picture on the right shows the various
performances indicated on the objectives.
Optical
performance
Objectives
Plan objective
(FN 20)
Glossary
Numerical aperture:
(NA)
Working distance:
(W.D.)
Resolution:
Field number:
(FN)
Total magnification:
Focal depth:
(Object side)
Actual field of view:
26
Working
Numerical
distance
Magnification
aperture
NA
4X
0.10
10X
0.25
20X
0.40
40X
0.65
100XO
1.25
The numerical aperture corresponds to F-number
resolution becomes higher when the numerical aperture becomes larger.
(
: F-number is a value obtained by dividing the focal distance of lens by the valid aperture diameter.
*
This is used as an index to show the brightness of the lens.)
Distance between the top surface of the cover glass and the tip of the objective.
The resolution is the ability of an objective to resolve adjacent two points in the image to the minimum
limit, which is expressed as the distance between two points on the specimen surface.
The field number is the diameter of the image viewed through eyepieces, indicated in millimeters.
Magnification of objective x Magnification of eyepiece
The focal depth is the depth range of the specimen focused. The depth becomes deeper by
narrowing down the aperture diaphragm and it becomes shallower by increasing the aperture
diaphragm of the objective.
The actual field of view is a diameter of the field of view, expressed as the size (mm) on the specimen
surface.
Magnification
Mechanical tube
length
Color band
Objective type (on the back side)
Plan objective
Cover
glass
Resolution
W.D.
thickness
(µm)
(mm)
(mm)
27.8
-
3.36
8.0
-
1.34
2.5
-
0.84
0.6
0.17
0.52
0.13
-
0.27
of the camera and is related to the resolution. The
*
Numerical aperture
Field number
Cover glass thickness
10X Eyepieces (FN 20)
Depth of
Actual
Total
focus
field of
magnification
(µm)
view
40X
175.0
5.0
100X
28.0
2.0
200X
9.27
1.1
400X
3.04
0.5
1000X
0.69
0.2
Remark
Oil immersed

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