Refractive Index Calibration - Agilent Technologies Infinity 1260 User Manual

8
Test Functions

Refractive Index Calibration

Refractive Index Calibration
Refractive Index Calibration
The refractive index calibration is based on a sucrose calibration solution,
which has a known refractive index compared to LC grade water. After both
the sample and reference cells have been purged with LC grade water the
sucrose solution is introduced into the flow cell and then the built-in
refractive index calibration functionality is used.
Filling the sample cell with the sucrose calibration solution will give a
theoretical detector response of 512,000 nRIU +/- 5,000 nRIU. The calibration
algorithm will allow the actual detector response, if different, to be changed to
the theoretical value.
Refractive index calibration is only required after exchange of the optical unit or the main
N O T E
(RIM) - board.
The Refractive Index Calibration Procedure
When Recommended after exchange of the optical unit or RIM board.
Tools required Laboratory balance
Parts required #
1
1
1
1
1
118
p/n Description
DAB/Ph Eur/BP/JP/NF/USP Grade Sucrose
9301-1446 Syringe
9301-0407 Needle
5061-3367 Sample filter
0100-1516 Fitting male PEEK, 2/pk
Agilent 1260 Infinity RID User Manual