Running A Checkout Sample - Agilent Technologies Infinity 1260 User Manual

4
Using the Refractive Index Detector

Running a Checkout Sample

Running a Checkout Sample

This chapter describes the check out of the Agilent 1260 Infinity Refractive
Index Detector using the Agilent isocratic checkout sample.
When If you want to checkout the detector
Parts required #
1
1
1 Turn the detector on.
2 Set up the instrument with the following chromatographic conditions.
Table 16
Mobile phases
Column
Sample
Flow rate
Stroke A
Stop time
Injection volume
Column compartment temperature
Optical unit Temperature
Polarity
Peak Width (Response time)
72
p/n Description
993967-902 Zorbax Eclipse XDB C18, 150mm x 4.6mm i.d.
01080-68704 Agilent isocratic checkout sample
You are now ready to change the settings of your detector.
Chromatographic Conditions
30% Water, 70% Acetonitrile
Zorbax Eclipse XDB C18, 150 mm x 4.6 mm i.d.
Isocratic standard sample
1.5 ml/min
20 µl
10 min
20 µl
25 °C
35 °C
Positive
0.2 min (4 s, standard)
Agilent 1260 Infinity RID User Manual