Step 3: Set Up Routine Methods - Agilent Technologies 1200 Series User Manual

4 First Steps with the Detector
Table 11
Column
Mobile phase
Gradient
Flow rate
Column temperature
Injection volume
FLD settings

Step 3: Set up routine methods

In routine analysis, sample matrices can have a significant influence on
retention times. For reliable results, sample preparation must be thorough to
avoid interferences or LC methods must be rugged enough. With difficult
matrices, simultaneous multi-wavelength detection offers more reliability than
timetable-controlled wavelength switching. The Agilent 1200 Series FLD can,
in addition, acquire fluorescence spectra while it records the detector signals
for quantitative analysis. Therefore qualitative data are available for peak
confirmation and purity checks in routine analysis.
74
Conditions for Figure 33 and Figure 34 on page 73
Zorbax SB, 2 x 50 mm, PNA, 5 µm
A = water; B = acetonitrile
0 minutes, 5%
10 minutes, 15%
0.4 ml/min
°
35 C
5 µl
PMT 12,
response time 4 s,
step size 5 nm
Ex 265 nm and 430 nm
Em 540 nm
1200 Series FLD User Manual