Step 3: Set Up Routine Methods; Fld Settings - Agilent Technologies 1100 Series Reference Manual

FLD settings

Step 3: Set up routine methods

In routine analysis, sample matrices can have a significant influence on retention times.
For reliable results, sample preparation must be thorough to avoid interferences or LC
methods must be rugged enough. With difficult matrices, simultaneous multi-wavelength
detection offers more reliability than timetable-controlled wavelength switching. The
Agilent 1100 Series FLD can, in addition, acquire fluorescence spectra while it records
the detector signals for quantitative analysis. Therefore qualitative data are available for
peak confirmation and purity checks in routine analysis.
1100 Series FD Reference Manual 49
First Steps with the Fluorescence Detector 2
PMT 12,
response time 4
s, step size 5 nm
Ex 265 nm and 430 nm
Em 540 nm