Solvent Reservoirs; General Precautions - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
SR-3000 Solvent Rack

2.5 Solvent Reservoirs

The solvent rack is shipped with solvent reservoirs and appropriate tubing, including filter
holders and filter frits.

2.5.1 General Precautions

When connecting the solvent reservoirs, observe the following general precautions:
• Rinse the eluent reservoirs thoroughly before using them for the first time. Use high-
purity solvent for rising.
• Always install filter frits on the solvent lines. This prevents contaminants from reaching
the HPLC system.
• Regularly check the filter frits for permeability. Replace the filter frits at regular intervals.
This is especially important when using aqueous solvents. Aqueous solvents may
contaminate the filters with algae and other microorganisms that deposit on the filter frits.
Therefore, also replace the solvents at regular intervals. Rinse the reservoirs thoroughly
before refilling them. Replace the filter frits as necessary.
• As standard, the filter frits shipped with the solvent rack are stainless steel frits.
If the UltiMate 3000 system includes a biocompatible pump, NCS-3500RS, or
NCP-3200RS, replace the stainless frits with the frits from the accessories kit of the
module. To do so, unscrew the top part of the filter holder from its bottom part and
replace the filter frit. When placing the new filter frit into the bottom part, make sure that
the frit is in a level position (avoid tilting the frit).
If the UltiMate 3000 system includes a semipreparative pump, use the filter holder and
filter frit from the accessories kit of the pump.
• Locate the solvent reservoirs as close as possible to the pump. To avoid formation of gas
bubbles in the reservoirs, make sure that the reservoirs are on the same level or higher as
the pump. Therefore, stack the rack onto the pump as shown in Fig. 1 (→ page 12). The
tubing connecting the pump to the degasser should be as short as possible to avoid
reformation of gas bubbles in the solvent.
• When replacing solvents, make sure that the solvents are miscible. Mix immiscible
solvents with an intermediate solvent (for example, isopropanol) to replace them step-by-
step.
Page 14 Operating Instructions