Related Manuals for Thermo Scientific Air-Jacketed Compact Flo-Thru 315
Summary of Contents for Thermo Scientific Air-Jacketed Compact Flo-Thru 315
- Page 1 Air-Jacketed Compact Flo-Thru ® Incubators Operator’s Manual and Parts List Model No. 315DV (dual voltage) 315-1 LT1448X1 • 5/28/09...
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Page 2: Table Of Contents
Table of Contents Safety Information ................................3 Alert Signals ................................3 Specifications ................................4 Power Requirements ............................4 Temperature Range ..............................4 Temperature Control ..............................4 Temperature Uniformity ............................4 Carbon Dioxide Range ............................4 Volume ..................................4 Overall Dimensions..............................4 Chamber Dimensions ............................4 Shelf Dimensions ..............................4 Shipping Weight ..............................4 Unit’s Environmental Operating Conditions ......................4 Unpacking and Installation ............................5 Shipping Carton ..............................5... -
Page 3: Safety Information
Safety Information Your Thermo Scientific Air-Jacketed Compact Flo-Thru ® Alert Signals CO2 Incubator has been designed with function, reliability, and safety in mind. It is your responsibility to install it in conformance with local electrical codes. It is most impor-... -
Page 4: Specifications
Specifications Power Requirements Overall Dimensions 315, 315DV (110): 120 Volts, 50/60 Hz, 200 Watts, 1.7 Amps 22.5”W x 18”D x 19”H (57cm x 46cm x 48cm) 315-1, 315DV (220): 240 Volts, 50/60 Hz, 200 Watts, 0.8 Amps Chamber Dimensions Temperature Range 14”W x 12”D x 12”H (36cm x 31cm x 31cm) Ambient +5°C to 60°C Shelf Dimensions... -
Page 5: Unpacking And Installation
Unpacking and Installation Shipping Carton The shipping carton should be inspected upon delivery. When received, carefully examine for any shipping dam- age before unpacking. If damage is discovered, the deliv- ering carrier should both specify and sign for the damage on your copy of the delivery receipt. -
Page 6: Gas Filter Interface Assembly Kit
NPACKING AND NSTALLATION Gas Filter Interface Assembly Kit Models 315, 315-1, and 315DV require this assembly to be used for both CO2 and air. Kit includes: • (4) Hose Clamps - #250-331-00 • (1) In-Line Filter - #525-034-00 • (1) 10 ft. (4.57 m) length of 1/4” (6.35 mm) ID PVC tubing - #720-418-00 Installation Procedure See next page for illustration. - Page 7 NPACKING AND NSTALLATION Figure A: Back of Unit – Rear Panel View, Exterior Back of unit In-line filter Hose clamp Hose clamp 1/4” (0.64 cm) ID tubing Hose clamp 1/4” (0.64 cm) hose barb connections (CO2 fitting) Pressure regulators and gauges must be installed on each tank.
- Page 8 Standard Type (Correct) Siphon Type (Incorrect) Compressed Gas Association #320 Warning tag indicating that cylinder is siphoning type. connector. Aluminum ring. Gas head space. Gold band. Liquid CO2 filled to 68% equal weight of water that cylinder would hold at Stamp or marking on cylinder: "Siphon"...
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Page 9: Description And Features
Description and Features Basic Components 1. Air and CO2 flowmeters 2. Unit feet 3. CO2 access port 4. Chamber shelves 5. Temperature sensor 6. Chamber gasket 7. Inner glass door, handle shaft 8. Inner glass door 9. Inner glass door, handle 10. - Page 10 ESCRIPTION AND EATURES Chamber Ceiling, Interior Circulating Fan Rear Panel View, Exterior Cordset Cordset CO2 inlet port Air inlet port Input connector 120/240 selector switch...
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Page 11: Description
ESCRIPTION AND EATURES Description CO2 levels are manually adjusted utilizing flowmeters. Flo- Thru control of CO2 concentrations and temperature create a growth environment ideal for tissue cultures, plants, cells, viruses and related organisms. An incoming gas mixture passes through the plenum and is gently circulated throughout the chamber. -
Page 12: Control Panel
ESCRIPTION AND EATURES Control Panel Temperature °C Temp Safety Temp Control Power Sample AIR FLOWMETER: Controls the amount of incoming air by rotating its control knob. LED DISPLAY: Displays current temperature. CALIBRATION POTENTIOMETER: Using a jeweler’s screwdriver, display can be adjusted. TEMPERATURE STATUS LAMP: Lit when heaters are active. -
Page 13: Operation
Operation Loading the Incubator Load the chamber, spacing the items as far apart as possible (for maximum air circulation). Air/Gas Ratio Set the flowmeters to obtain the required air and CO2 flow— using the lowest possible CO2 flow rate will help to conserve gas. -
Page 14: Co2/Air Flow Nomograph: 315 Series Flo-Thru Co2 Incubators
PERATION CO2/Air Flow Nomograph: 315 Series Flo-Thru CO2 Incubators 26 24 22 20 181614 12 10 CO2 10 1.5 2 2.5 3 4 5 8 9 10 15 20 25 30 40 LITERS PER MINUTE, AIR 1. DRAW A VERTICAL LINE FROM THE POINT THAT REPRESENTS THE AIR FLOW ON THE HORIZON- TAL AXIS UNTIL IT INTERSECTS A DIAGONAL LINE INDICATING THE REQUIRED VOLUME (%) OF CO2 FLOW;... -
Page 15: Thermostat Control
PERATION Thermostat Control A hydraulic thermostat is utilized for set point control to protect chamber from over-temperature conditions. Operating Suggestions, 315 Series Flo-Thru CO2 Incubators • In order to obtain and maintain the desired CO2 gas pressure, periodically check the tightness and integrity of the gasket that secures the glass door to the chamber. -
Page 16: Temperature Readout Calibration
PERATION Temperature Readout Calibration Place a scientifically accurate thermometer in the cham- ber, on a center shelf and allow the incubator to stabilize at some set temperature. If the digital temperature read- out does not agree with the thermometer, take the trim- mer pot tool, insert in calibration pot on control panel and adjust until readout matches the measured temperature. -
Page 17: Using Fyrite
Using Fyrite Hints on Using the Fyrite The following information is intended to supplement that found in the Fyrite manual and is based on experience working with users of our Incubators. One of the most important aspects of using the Fyrite pro- cedure and one that is frequently overlooked is the condi- tion of the fluid. -
Page 18: Taking A Fyrite Reading
SING YRITE drops of distilled water into the plunger on the top of the Fyrite. Depress the plunger and the water will transfer into the Fyrite causing the fluid level to rise. Repeat this procedure until the fluid level reads correctly. •... - Page 19 SING YRITE of the bubbles surface and the fluid has filled the head. Even the smallest bubbles may contain CO2, so that it is important to let most of the smaller bubbles surface. Turn it right side up and hold it at 45 degrees. Allow the fluid to fill the Fyrite and depress the plunger again.
- Page 20 SING YRITE complete and thorough transfer of the sample to the Fyrite chamber. 11. Once the bulb has been pumped the required number of times, HOLD THE SQUEEZE BULB IN THE "SQUEEZED" POSITION and release the pressure on the cup that will also release the plunger.
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Page 21: Maintenance
Maintenance Cleaning Warning Every 6 months inspect the chamber. Clean the stainless Disconnect from the power supply steel interior with any good scale remover or dilute acetic acid prior to maintenance and servicing. and a synthetic scouring pad. DO NOT USE chlorine-based cleansers or bleach, scouring pads with metallic content, or harsh abrasives to clean any part of the Incubator. -
Page 22: The Ph Factor
AINTENANCE The pH Factor Check pH regularly. If pH is <6.0, add disodium phos- phate to increase pH to a 7 to 9 value. Sodium carbonate or sodium bicarbonate may be used but they tend to form scale that must be rinsed out regularly. If pH is >10.0, add sodium bisulfate to decrease pH to a 7 to 9 value. -
Page 23: Cleansing Agents
AINTENANCE Cleansing Agents Anti-fungal and anti-bacterial additives are permissible to use as long as the pH of the aqueous solution is kept within the range of 7 to 9. These are available through laboratory distributors. Be sure to CONFIRM that they are not harmful to stainless steel. -
Page 24: Materials Effective In Disinfecting
AINTENANCE • Oxalic acid 2% to 5% in warm water. Swab solution on surface allowing it to remain until rust is loosened. Immediately follow with a clean water rinse. This method should ONLY be used if SEVERE rust and scale stains are present. Regardless of the approach utilized, ALWAYS follow the manufacturer’s directions and allow the chemicals to do the cleaning with MINIMAL scrubbing. -
Page 25: Replacement Parts
Replacement Parts 315 SERIES FLO-THRU CO2 INCUBATORS: DESCRIPTION PART NUMBER Thermostat MOV: CA1447X5 Heater: EL1447X4 Glass Door: 540-228-00 Door Gasket: GS1447X1 240V Euro Line Cord: 470-306-00 120V Domesticated Line Cord: 470-262-00 Foot: 790-225-00 Shelf, 315 Series (2): 810-424-02 Flowmeter - Air: 660-044-00 Flowmeter - CO2: 660-043-00... -
Page 26: Ordering Procedures
Ordering Procedures Please refer to the Specification Plate for the complete model number, serial number, and series number when requesting service, replacement parts or in any corre- spondence concerning this unit. All parts listed herein may be ordered from the Thermo Scientific dealer from whom you purchased this unit or can be obtained promptly from the factory. -
Page 28: Two Year Limited Warranty
(ii) misuse (including use inconsistent with written operating instructions for the product), mishandling, contamination, overheating, modification or alteration of the product by any customer or third party or (iii) use of replacement parts that are obtained from a party who is not an authorized dealer of Thermo Scientific prod- ucts.