Before Cultivation; Preparing And Autoclaving The Culture Vessel; Checking Gaskets (O-Rings) - Infors HT Minifors 2 Operating Manual

Minifors 2 - Benchtop Bioreactor

Before Cultivation

8 Before Cultivation

8.1 Preparing and Autoclaving the Culture Vessel

8.1.1

Checking Gaskets (O-Rings)

Page 74 of 214
The following chapters describe all the preparatory work before
starting the cultivation process. This essentially comprises:
Preparing and autoclaving the culture vessel:

Checking the gaskets (O-rings) on component parts and

culture vessel
Mounting component parts

Filling or moistening the culture vessel

Preparing sensors and other accessories

Autoclaving

Connecting the culture vessel and preparing for cultivation:

Hanging the culture vessel into place on the basic unit

and connecting cables and hoses between the culture
vessel and the equipment
Filling the vessel if necessary

Preparing sensors and other accessories

All accessories required for later cultivation must be prepared and
mounted accordingly and autoclaved together with the culture ves-
sel.
Certain accessories are already mounted when the equpment is
delivered.
O-rings are used to seal all openings on the vessel and top plate.
The top plate and all internal fittings or connections are thus
equipped with O-rings. Before every use, the O-rings must be
checked that they are present, undamaged and correctly seated.
Damaged O-rings must be replaced.
INFORMATION
Wet the O-rings with 70% alcohol or a little water to facilitate re-
moving and replacing O-rings or component parts with O-rings.
Do not use silicone grease; this can affect sterilisation results.
Carry out this check as follows:
07 February 2019