Maintenance - Thermo Scientific F1-ClipTip User Manual

The factor Z is for converting the weight of the water to volume at the test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table in Appendix 1.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V
A = inaccuracy
0
V = mean volume
V = nominal volume
0
Inaccuracy can be expressed as a relative value: A% = 100% x A / V
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (S) or
coefficient of variation (CV)
Standard deviation can be expressed as a relative value: CV = 100% x S / V
Cautions for incorrect maintenance
The pipette should be regularly serviced according to the instructions for use.
The pipette performance and functioning must be checked after maintenance.
Aggressive substances can damage the pipette or pipette parts. Check for material
compatibility before using organic solvents and aggressive chemicals.
Use only original Thermo Scientific spare parts and ClipTip tips.
Avoid excess grease. Use only the grease provided with the pipette.
Annual pipette usage varies significantly among laboratories, and the types of substances being
aspirated and dispensed also vary tremendously among laboratories. In order to maintain proper
care of the pipette, it is the laboratory's responsibility to develop a proper maintenance program
that meets its specific needs and requirements.
When the F1-ClipTip is not in use, make sure it is stored in an upright position. We recommend
a pipette stand for this purpose.
When shipping the device for service or inspection, make sure that it is completely free of
chemical, biological, or radioactive contamination.
7.1 Daily maintenance
The pipette should be checked at the beginning of each day for dust and dirt on its outside surfaces.
Particular attention should be paid to the tip cone. To clean the pipette, we recommend wiping it with
a napless cloth wetted with 70% ethanol.
Leakage test
A leakage test can be performed at any time. It is recommended to be carried out after maintenance
or autoclaving.
Pre-wet the tip three to five times with water.
Aspirate a nominal volume of water into the tip.
Place the pipette on a stand or keep it firmly in your hand.
Wait for 20 seconds.
If a drop falls within 20 s, the pipette is leaking.
If the pipette leaks, see Chapter 9: "Troubleshooting" for possible reasons and solutions.
7.2 Periodic maintenance
The maintenance procedure starts with the disassembly of the pipette.
F1-ClipTip O-rings 5.1 are wearing parts. It is recommended to replace O-rings regularly to
ensure proper sealing. Always replace the O-rings if they are worn or damaged.
S = standards deviation
V = mean volume
n = number of measurements

7. Maintenance

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