Thermo Scientific F1-ClipTip 4641170 Instructions For Use Manual
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Thermo Scientific F1-ClipTip 4641170 Instructions For Use Manual

Single channel; multichannel
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Thermo Scientifi c F1-ClipTip
Single Channel
Multichannel
Instructions for Use
1

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Summary of Contents for Thermo Scientific F1-ClipTip 4641170

  • Page 1 Thermo Scientifi c F1-ClipTip Single Channel Multichannel Instructions for Use...
  • Page 2 This product complies with the European Union Directive 98/79/EC, and it is marked with a CE marking. When the product is used in applications related to the Directive 98/79/EC, read the additional information enclosed in the product package or contact the manufacturer at info.pipettes@thermofi...

  • Page 3: Table Of Contents

    CONTENTS 1. INTRODUCTION 2. PACKAGE 3. MAIN ILLUSTRATION 4. SAFETY 5. OPERATION 6. CALIBRATION AND ADJUSTMENT 7. MAINTENANCE 8. STERILIZATION 9. TROUBLESHOOTING 10. APPENDICES...

  • Page 4: Introduction

    1. Introduction Congratulations on your purchase of a Thermo Scientifi c F1-ClipTip pipette! ® ® The F1-ClipTip is a general purpose air displacement micropipette. Its intended use is for aspirating and dispensing liquids. The F1-ClipTip features an innovative interlocking tip interface technology that locks the tip in place ensuring secure tip attachment and preventing tips from being displaced in the middle of pipetting.

  • Page 5: Package

    2. Package The F1-ClipTip package contains the following items: F1-ClipTip pipette Service tool Multichannel service tool Tip fi tting tool ClipTip tip sample Tube of grease (Order No. 2203130) Instructions for Use Calibration certifi cate / Warranty Certifi cate Shelf hanger (Order No. 2206040) 10.

  • Page 6: Safety

    3.1 . Materials The F1-ClipTip is made of mechanically durable and chemically resistant materials. The F1-ClipTip pipette includes components which contain the antimicrobial additive, silver sodium hydrogen zirconium phosphate. Silver is known to inhibit the growth of a broad spectrum of micro- organisms.

  • Page 7: Operation

    Incorrect dispensing results Performance may vary due to: a. pipetting method (forward pipetting technique recommended) b. temperature (air, liquid, vessel, pipette, and tip) c. pressure d. humidity e. operator, e.g. thumb movement, pipetting angle f. liquid density, viscosity and vapor pressure g.
  • Page 8 5.3 Setting the delivery volume 1. Set the delivery volume using the volume adjustment button on the top of the pipette (Picture 3). Pull the button to activate the volume setting. 2. To increase the delivery volume, turn the button counterclockwise.

  • Page 9: Calibration And Adjustment

    Picture 6 Plunger in up position Plunger in down position When the pipette is not used for a period of time, the plunger movement may become stiff due to hardening of grease. In rare cases this can affect the functionality of the super blow-out piston, which can become temporarily jammed.
  • Page 10 6.3 Procedure to check calibration The pipette is checked at maximum volume (nominal volume) and minimum volume. A new tip is fi rst pre-wetted three to fi ve times and a series of ten pipettings is done at both volumes. A pipette is always adjusted according to the delivery of the selected volume.

  • Page 11: Maintenance

    7. Maintenance Cautions for incorrect maintenance The pipette should be regularly serviced according to the instructions for use. The pipette performance and functioning must be checked after maintenance. Aggressive substances can damage the pipette or pipette parts. Check for material compatibility before using organic solvents and aggressive chemicals.
  • Page 12 Picture 10 2. Press the tip ejector 4.1. Rotate the tip ejector 10 counterclockwise and pull it out (Picture 11). 3. Turn the tip cone 11 counterclockwise with the service tool 1. There are two slots in the service tool: the smaller one for pipettes under 300 μl and the bigger one for pipettes over 300 μl.
  • Page 13 3. Remove the Clip trigger 8 and Clip trigger spring 9. 4. Turn the tip cone 11 counterclockwise with the service tool 1. There are two slots in the service tool: the smaller one for pipettes under 300 μl and the bigger one for pipettes over 300 μl.
  • Page 14 100-1000μl, 500 / 1000μl Fixed Put the O-ring 17 and support ring 16 onto the tip cone 11. Slide the spring 13 onto the piston 12 and slide the entire assembly into the tip cone 11. Put the spring 15 and support 14 on top of the tip cone and carefully insert the tip cone assembly to the handle.
  • Page 15 6. Remove the two screws 19 and pull the tip ejector 23 into the down position as shown in the picture on page 25. 7. While the tip ejector 23 is in the down position, open its upper end slightly on both sides and pull downwards.

  • Page 16: Sterilization

    7.2.7 Cleaning and reassembling multichannel pipettes See the cleaning instructions in Section 7.2.2 “Single channel pipettes”. Check the volume-specifi c reassembling of the piston assemblies starting on page 23. The pipette is assembled in the reverse order to disassembling. Note that the ejector link 24 must be in the down position simultaneously with the tip ejector 23 as shown in the picture on page 25.

  • Page 17: Troubleshooting

    9. Troubleshooting The table below lists possible problems and their solutions. Defect Possible reason Possible action ►Re-attach/tighten the tip fi tting Leakage • Tip fi tting incorrectly attached or loose or replace with new tip fi tting(s) using the tool. ►Discard the tip.

  • Page 18: Appendices

    10. Appendices Appendix 1. Conversion table Value of the conversion factor Z (μl/mg), as a function of temperature and pressure, for distilled water. Temperature Air pressure °C 101.3 15.0 1.0017 1.0018 1.0019 1.0019 1.0020 1.0020 1.0020 15.5 1.0018 1.0019 1.0019 1.0020 1.0020 1.0020...
  • Page 19 Appendix 2. Manufacturer specifi cation limits Thermo Scientifi c F1-ClipTip Variable Volume Single Channel Compatible Order No. Range Volume Inaccuracy Imprecision ClipTip µl µl SD µl 4641170 1–10 µl ±0.100 ±1.00 0.050 0.50 ±0.075 ±1.50 0.040 0.80 ±0.025 ±2.50 0.020 2.00 4641180 2–20 µl...
  • Page 20 Thermo Scientifi c F1-ClipTip Variable Volume Multichannel Compatible Order No. Range Channel Volume Inaccuracy Imprecision ClipTip µl µl SD µl 4661110 1–10 µl ±0.240 ±2.40 0.160 1.60 ±0.200 ±4.00 0.150 3.00 ±0.120 ±12.00 0.080 8.00 4661120 5–50 µl ±0.75 ±1.50 0.35 0.70 ±0.625...
  • Page 21 Appendix 3. ISO8655 calibration specifi cations Thermo Scientifi c F1-ClipTip specifi cations according to ISO8655 standard. Thermo Scientifi c F1-ClipTip Variable Volume Single Channel Compatible Range Volume Inaccuracy Imprecision ClipTip Color code µl µl SD µl 1–10 µl ±0.120 ±1.2 0.080 pink ±0.120...
  • Page 22 Appendix 4. Assembly and spare parts * Available as a spare part. See model specifi c order numbers on page 27. 1062930 1062800 Service and Multichannel service tool adjustment tool 2215870 Tip fitting tool * 4. Handle * 5. Tip fitting * 42.
  • Page 23 1–10μl 2–20μl 5–50μl 1μl / 5μl / 10μl Fixed 20 μl Fixed 25 / 50 μl Fixed 1–300μl 5.2* 5.1*...
  • Page 24 100–1000μl 20–200μl 30–300μl 500 / 1000 μl Fixed 100 / 200 μl Fixed 10–100μl 1–300μl 250μl Fixed 5.2* 5.2* 5.1* 5.1*...
  • Page 25 * 50 5.2* 5.1*...
  • Page 26 5.2* 5.1*...
  • Page 27 F1-ClipTip single channel and multichannel spare part order numbers Single Channel 1–10μl / 1μl / 5μl / 10μl Fixed 30–300μl / 250μl Fixed 2214000S 1-10μl 2214050S 30–300μl 2214070S Fix 1μl 2214150S Fix 250μl 2214080S Fix 5μl 11072080 2214090S Fix 10μl 5.1.
  • Page 28 Appendix 5. ClipTip ordering information Thermo Scientifi c ClipTip racked and sterile tips Code ClipTip Volume 94410210 ClipTip 20 20 μl 10x96/rack 94410213 ClipTip 20, sterile 20 μl 10x96/rack 94410250 ClipTip 50 50 μl 10x96/rack 94410253 ClipTip 50, sterile 50 μl 10x96/rack 94410310 ClipTip 200...
  • Page 29 Appendix 6. F1-ClipTip and ClipTip compatibility table Non filter tips Filter tips F1 ClipTip 1 10 l F1 ClipTip 2 20 l F1 ClipTip 5 50 l F1 ClipTip 10 100 l F1 ClipTip 20 200 l F1 ClipTip 30 300 l F1 ClipTip 100 1000 l F1 ClipTip 1 l fixed volume F1 ClipTip 5 l fixed volume...
  • Page 30 Appendix 7. Pipette accessories Multichannel stand #9420390 F–stand #9420400 Reagent reservoir 100 ml #95128085 Reagent reservoir 25 ml #95128093 Reagent reservoir 25 ml divided #951228095...
  • Page 31 info.pipettes@thermofi sher.com www.thermoscientifi c.com/GLP www.thermoscientifi c.com thermoscientific.com © 2015 Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientifi c Inc. and its subsidiaries. Specifi cations, terms and pricing are subject to change. Not all products are available in all countries.

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