Calibration And Adjustment - Thermo Scientific F1-ClipTip User Manual

Withdraw the tip from the liquid by moving
it against the edge of the reservoir to
remove excess liquid.
4. Deliver the liquid by gently depressing the
button to the first stop. After a delay of
about one second, continue to depress the
button all the way to the second stop. This action
will empty the tip.
5. Withdraw the tip by moving it against the edge of
the target container.
Ensure proper functioning of 2-20µl single channel and 5-50µl pipettes
The F1-ClipTip 2-20µl single channel pipette and 5-50µl pipettes utilize an innovative super blow-out
piston technology to ensure efficient liquid delivery with small volumes. In pipettes with the super
blow-out piston three separate movements are observed (Picture 6) when the plunger is pressed
down to dispense liquid:
1. Primary plunger movement – delivers the set volume amount
2. Blow-out – additional phase to dispense residual liquid
3. Super blow-out – enhances the effectiveness of blow-out
1
2
3
Picture 6
When the pipette is not used for a period of time, the plunger movement may become stiff due to
hardening of grease. In rare cases this can affect the functionality of the super blow-out piston,
which can become temporarily jammed. To ensure proper functionality it is recommended to press
the plunger several times to the final stop (step 3 in picture) before dosing the sample liquid. This
releases the piston and re-distributes the lubricant.
NOTE! If the super blow-out piston is jammed, increased force might be required to release it. Ensure
that plunger moves to the down position (Picture 6).
For more information about different pipetting techniques, check the Good Laboratory Pipetting
Guide online.
5.6 Tip ejection
To release the tip, point the pipette at a suitable waste receptacle and press the tip ejector with
your thumb.

6. Calibration and Adjustment

6.1 Factory calibration limits
F1-ClipTip pipettes are factory calibrated and adjusted to give the volumes as specified with distilled
or deionized water using the forward pipetting technique. Manufacturer specifications are shown on
the pipette calibration certificate and in Appendix 2. The pipette should be recalibrated for use with
any tip type not named on the Calibration Certificate.
Cautions for calibration and pipette adjustment
It should be noted that the use of different pipetting techniques may affect the calibration
results. The pipettes are constructed to permit re-adjustment for other pipetting techniques,
types of tips, or liquids of a different temperature or viscosity.
Plunger in up position
9
1 2 3 4
Picture 5
Plunger in down position