Mass Calibration; Em Gain Calibration; Tune And Mass Calibration; Figure 31. Ion Signal Intensity As A Function Of The Lens L0 Offset Voltage - Thermo Scientific TSQ Altis Hardware Manual

Mass Calibration

EM Gain Calibration

Tune and Mass Calibration

Figure 31. Ion signal intensity as a function of the lens L0 offset voltage

Thermo Scientific
Use the Mass Calibration option
During the mass position and resolution evaluation, the calibration procedure varies the RF
and DC voltages that it applies to quadrupoles Q1 and Q3 to best fit the measured peak
widths and positions of the EMRS calibrant ions. This procedure repeats for FWHM peak
widths of 0.2 Da (TSQ Altis MS only), 0.4 Da, and 0.7 Da; for three different scan rates
(250, 500, and 1000 Da/sec); and for quadrupoles Q1 and Q3.
If you notice a falloff of the ion signal intensity, use the EM Gain Calibration option
(Figure 28) to increases the voltage on the electron multiplier. This will maintain a gain of
5
1 × 10 for MS mode and 1 × 10
When you select the Tune and Mass Calibration option
ion signal by optimizing the DC offset voltages of the lenses and other optical elements.
Figure 31 shows the lens L0 offset voltage optimization for EMRS. After the optimizations are
complete, the system performs a full mass position and resolution calibration.
(Figure 28) if the Check Mass Calibration option fails.
6
for MS/MS mode.
(Figure
TSQ Altis, Quantis, and Fortis Hardware Manual
15
Calibrating the MS
Calibrating and Tuning the MS
28), the MS maximizes the
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