Operation - Integra VACUSAFE Operating Instructions Manual

VACUSAFE – Operating instructions V12
3.3.4 Connection and calibration of the level sensor
In case of failed calibration (indicated by continuous beeping and blinking of the switch
indicator lamp), repeat the calibration after cleaning the sensor pins inside the lid and
checking that the level sensor cable is fully inserted in the lid and base.
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Operation

4.1 Vacuum activation and notes on use
Switch on the VACUSAFE to start the vacuum pump (the switch indicator lamp lights).
Wait a few seconds until the air from the collection bottle is evacuated. To increase or
decrease aspiration speed, turn the knob for vacuum regulation to the right or left,
respectively (see "2.2 Overview of the VACUSAFE" on page 5).
Aspirate liquid by pressing the green button of the VACUBOY hand operator fitted with a
suitable tip. Check the level of liquid in the bottle frequently, especially when working
without level sensor, and make sure that there is enough space left for the liquid that needs
to be aspirated.
To open the bottle, first release the vacuum by opening the venting port on the lid (open
the sealing screw or remove the sealing cap). Alternatively you can aspirate air with the
VACUBOY. When closing the bottle for use, screw the lid on tightly. The seal in the lid
needs to be adequately compressed in order to function. Also make sure the venting port
is closed.
Prevent liquid or foam overflow from the collection bottle into the filter. In case the filter
gets accidentally wetted or soiled, exchange it immediately or otherwise the retention of
further liquid cannot be guaranteed. Note that the hydrophobic filter holds back aqueous
solutions, whereas retention of apolar liquids is limited. To prevent the build-up of foam,
we recommend the use of an anti-foaming agent (e.g. Antifoam A from Sigma).
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1) Plug the level sensor cable into its socket at the back of
the instrument and connect it to the bottle lid (models
158300, 158310). Make sure that the sensor pins inside
the lid are dry and unsoiled.
2) Switch on the VACUSAFE. A double beep confirms
correct connection of the level sensor.
3) Press the "C
ALIBRATION
VACUSAFE base until a short beep is emitted.
Operation
" button on the back of the
INTEGRA Biosciences AG