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Table of Contents
Related Manuals for Integra VIAFLO Series
Summary of Contents for Integra VIAFLO Series
- Page 1 VIAFLO Electronic Pipettes Operating instructions 900-00002-00_V05...
- Page 2 Déclaration de conformité | Declaración de conformidad | Dichiarazione di conformità INTEGRA Biosciences – 2 Wentworth Drive, Hudson, New Hampshire 03051 declares on its own responsibility that the product | erklärt in alleiniger Verantwortung, dass das Produkt | déclare sous sa responsabilité exclusive, que le produit | declara bajo su propia responsabilidad que el producto | dichiara sotto la propria responsabilità...
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Page 3: Table Of Contents
3.2.1 Charging the battery on a stand..........14 3.2.2 Charging the battery with UPS..........15 3.3 Toolbox - adapt your VIAFLO Electronic Pipettes ......15 3.3.1 Preferences.................16 3.3.2 Calibration & Services............17 3.3.3 Communications ..............18 3.3.4 Owner Information ..............18 3.3.5 Help Language..............19 3.3.6 Write protect................19 3.3.7 Password key..............19 www.integra-biosciences.com... - Page 4 5.2.10 Serial dilution mode ............40 5.3 Custom step-based programming mode ........41 5.3.1 Create a custom program ...........41 5.3.2 Program tip adjustments in a custom program ....42 5.3.3 Modify existing programs ............42 5.3.4 Example of custom mode ...........43 INTEGRA Biosciences AG...
- Page 5 6.1.1 Assembly of single channel pipettes........44 6.1.2 Assembly of multichannel fixed spacing pipettes....46 6.2 Sterilization ..................48 6.2.1 Autoclaving the disassembled components......49 6.3 Servicing..................49 6.3.1 Shipping to INTEGRA Biosciences........49 6.3.2 Changing O-rings of tip fittings..........49 6.3.3 Lubrication ................50 6.4 Calibration ..................51 6.4.1 Materials ................51 6.4.2 Definitions ................51...
- Page 6 © 2013 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
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Page 7: Chapter 1 Introduction
This symbol identifies important notes regarding the correct operation of the device and labour-saving features. Intended use VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5 - 5000 µl using GripTip pipette tips. www.integra-biosciences.com... -
Page 8: Safety Notes
Do not open or modify the VIAFLO Electronic Pipettes in any way. Repairs may only be performed by INTEGRA Biosciences AG or by an authorised after-sales service member. Parts may be replaced with original INTEGRA Biosciences parts only. -
Page 9: Description Of The Device
VIAFLO single and multichannel pipettes couple an easy-to-use touch wheel controller with intuitive programming. They are microprocessor controlled and stepper motor driven. In addition, the VIAFLO VOYAGER adjustable tip spacing pipettes provides you with the ability to vary tip spacing. www.integra-biosciences.com... -
Page 10: Overview Of The Viaflo Electronic Pipettes
6 Purge button, to empty tips 7 Run key, to start operations 8 Tip ejector 9 Finger hook, facilitates easy operation 10 Volume indicator label, color matches GripTip rack insert. 11 Ejector sleeve 12 Tri-lobe tip interface INTEGRA Biosciences AG... -
Page 11: Back View
VIAFLO Pipettes – Operating instructions V05 Description of the device 2.3.2 Back view 13 Reset button 14 Power connector 15 Charging stand interface 16 Battery 2.3.3 Display The Display shows all pipetting options. Battery life indicator Current mode Instruction Active step Volumes Options www.integra-biosciences.com... -
Page 12: Touch Wheel
Press and release the Run key to initiate aspiration, dispense, mix, purge, and special pipetting operations. This button is centrally located for left- or right-handed actuation. During dispense, you can press and hold the Run key to perform a two-step blowout, see “4.3.2 Blowout modes” on page 21. INTEGRA Biosciences AG... -
Page 13: Tip Ejector
RAM in the pipette. The programs stored in memory are maintained. Once pressed, the VIAFLO startup screen will be displayed. Press any key to continue and allow the instrument to initialize and home the pipette. The routine ends by displaying the Main Menu. www.integra-biosciences.com... -
Page 14: Chapter 3 Installation
• Three static grey bars: Battery is fully charged. AUTION Use only the approved INTEGRA battery, power supply or charging stand. Use of an incompatible power transformer can damage the pipette. Battery charging can take place in two ways, using a pipette charging stand or the Universal Voltage Power Supply (UPS), that allows for pipetting while charging the battery, see (“8.1 Accessories”... -
Page 15: Charging The Battery With Ups
Enables communication between your VIAFLO Electronic Pipettes and a PC. Owner Information View your pipettor’s serial number and set a personal ID. Help Language Sets language specific help. Write Protect Protects programs or menu options from modification. Password Key Displays the encrypted password. www.integra-biosciences.com... -
Page 16: Preferences
(delay before the piston homes) at the end of a dispense, if no two step blowout is performed, see “4.3.2 Blowout modes” on page 21. After changing desired settings, press to Save. INTEGRA Biosciences AG... -
Page 17: Calibration & Services
Service His- Displays notes of any service that took place on the tory VIAFLO Electronic Pipettes listed newest entry first. Calibrate Tip Starts calibration of tip spacing of VOYAGER pipettes. Spacing After changing desired settings, press to Save. www.integra-biosciences.com... -
Page 18: Communications
VIALINK is a pipette management software for the PC and is used to manage VIAFLO Electronic Pipettes. It can be downloaded from the INTEGRA website in the product section and is free of charge for all VIAFLO Electronic Pipettes customers. A detailed description of the software, along with the operating instructions, can be found on the website as well. -
Page 19: Help Language
Keep the password in a safe place. 3.3.7 Password key This menu displays the encrypted password, if set. Should you lose your password, contact INTEGRA Biosciences to retrieve your password using these numbers. www.integra-biosciences.com... -
Page 20: Chapter 4 Operation
Tri-lobes minimize contact surface and temporarily deforms the tip shaft Shoulder provides a positive stop to prevent over tightening and all tips seal at the same height O-ring provides forgiving and robust seal surface for the pipet tip INTEGRA Biosciences AG... -
Page 21: Start Pipetting
• Two-step blowout: Perform a two-step blowout to manually delay the blowin: - Press and hold the Run key to start dispense with blowout. - Remove the tips from the target vessel. - Release the Run key to start blowin. www.integra-biosciences.com... -
Page 22: Recommendations For Pipetting
4.3.3 Recommendations for pipetting INTEGRA Biosciences recommends the following techniques for enhancing pipetting results. These techniques are consistent with ISO standard 8655-2. • It is best to immerse the GripTips just enough in liquid (2-3 mm) to allow the desired volume to be aspirated. -
Page 23: Pipetting Options And Settings
• When in other modes, select the Edit option and press OK. The steps with volumes to be adjusted are displayed. Use the Touch wheel to highlight the desired volume and press Favorites to display the list of favorite volumes. www.integra-biosciences.com... -
Page 24: Speed Selection
Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting. To dispense liquids with low viscosity and high vapor pressure, such as alcohol, use relative fast pipetting speeds and avoid prolonged pauses for aspiration. INTEGRA Biosciences AG... -
Page 25: Pace
The Help information available for each mode describes the mode operation. You can select Help in two ways: While in the Main menu, highlight a pipet mode, then press to select the Help option. While in a pipet mode, find the Help option from the list and press OK. www.integra-biosciences.com... -
Page 26: Voyager Tip Spacing
When the desired tip spacing is achieved, press OK. Make the desired changes to all positions. Upon completion, press to Save all the selections. INTEGRA Biosciences AG... -
Page 27: Tip Spacing Operation
Every time the pipette is turned on, the tip spacing motor homes and returns to the position that it was last in. You also may home the tip spacing motor as needed. To do so, press on the Tip Spacing menu to select the Home Tips option www.integra-biosciences.com... -
Page 28: Troubleshooting/Faq
• O-ring not installed. Droplets on the • Temperature of liquid differs • Pre-wet tips up to 3 times. tips. from that of air inside the tips. • Liquid of low viscosity and • Increase dispensing speed. high vapor pressure. INTEGRA Biosciences AG... -
Page 29: Electronic
Error message is • Indicates too much friction • Reset the pipette. displayed: “Home was encountered during • If problem persists, please con- Found” or “Home operation. tact service for technical assis- Not Found.” • Indicates possible motor fail- tance. ure. www.integra-biosciences.com... - Page 30 Motor sounds rough • Pipette has been set to the • Verify the speed setting. The and aspiration is slowest speed. slowest speed setting normally very slow. causes the motor to run slowly and louder than normal. INTEGRA Biosciences AG...
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Page 31: Pipetting Modes
Allows aspirating a transfer volume followed by a mix. Columns and Mix Cycles are tracked on the display. Custom Allows to create and store of up to 20 multi-stepped pipetting protocols. Press the OK to access the pipetting mode and to start defining parameters. www.integra-biosciences.com... -
Page 32: Detailed Description Of Pipetting Modes
• With the tip(s) in the destination plate, press and hold the Run key to execute the dis- pense and perform a two-step blowout, see “4.3.2 Blowout modes” on page 21. • When the tips are removed from the target plate, release the Run key. INTEGRA Biosciences AG... -
Page 33: Repeat Dispense Mode
• The pipettor will stop paced dispenses when it reaches the Last Dispense. This aliquot contains the accumulated error from all prior dispenses. You can choose to use this Last Dispense or discard it. During the Last dispense, press and hold the Run key to perform a two-step blowout. www.integra-biosciences.com... -
Page 34: Sample Dilute Mode
• Press and release the Run key to dispense. Mixing occurs automatically after the dis- pense step. • Upon completing the desired number of mixes, a blowout is initiated automatically prompting you to remove the tip(s) from the liquid and press Run to complete the blowout. INTEGRA Biosciences AG... -
Page 35: Manual Pipet Mode
The notation on the display changes between (Aspirate) and (Dispense). • Titrations can be performed by dispensing in this mode. The volume remaining in the tip(s) is always actively displayed. Use slower pipetting speeds (1-5) for better control and resolution. www.integra-biosciences.com... -
Page 36: Reverse Pipet Mode
- Press and release the Run key: Finish the aspirate and dispense process. The programmed volume will be dispensed. Last Dispense is the next active step. • To purge the Last Dispense volume with a two-step blowout, press and hold the Run key. INTEGRA Biosciences AG... -
Page 37: Variable Dispense Mode
Last Dispense. This aliquot contains the accumulated error from all prior dispenses. You can choose to use this Last Dispense or discard it. • During the Last dispense, press and hold the Run key to perform a two-step blowout. www.integra-biosciences.com... -
Page 38: Variable Aspirate Mode
• With the tip(s) in liquid, press and release the Run key to initiate the first aspiration volume. Again in liquid, press and release the Run key to initiate the second aspira- tion volume, etc. • Press and hold the Run key to start Dispense and perform a two-step blowout. INTEGRA Biosciences AG... -
Page 39: Sample Dilute/Mix Mode
• Press and release the Run key to dispense the entire tip contents and begin the mix- ing routine. Upon completing the desired number of mixes, a blowout occurs automat- ically. Remove tips from liquid and press and release the Run key to complete the blowout. www.integra-biosciences.com... -
Page 40: 5.2.10 Serial Dilution Mode
Run key to start the dispense and mix sequence. Proceed with the rest of the rows. • Rows (first number) and Mix Cycles (second number) are tracked on the display. Mix Cycles are shown in red when mixing. A green dot on the row number indicates the active program step. INTEGRA Biosciences AG... -
Page 41: Custom Step-Based Programming Mode
When starting with an “Aspirate” step followed by a “Mix” step, the tips contain the aspirate volume after completing the last mix cycle. When starting with a “Mix” step, the tips are emptied upon completion of the last mixing cycle. www.integra-biosciences.com... -
Page 42: Program Tip Adjustments In A Custom Program
At the Custom program display, use the Touch wheel to highlight an existing program. Press the OK button to select it. Select an option (View/Edit, Delete, Copy, Name) to modify the program. Press Back to return to the list of Custom programs. To run the program, press . INTEGRA Biosciences AG... -
Page 43: Example Of Custom Mode
Purge does not need to be programmed. The residual liquid is dispensed into the waste container. Press and hold Run key until liquid is purged and tips are removed from the liquid (two-step blowout). For a detailed description see “4.3.2 Blowout modes” on page 21. www.integra-biosciences.com... -
Page 44: Chapter 6 Maintenance
2) Turn off power and unplug the power supply. 3) Unscrew clockwise to remove the Cylinder Assembly (a) from the pipette. Slide the Cylinder Assembly down from the pipette body. This will expose the Piston Assembly (b) INTEGRA Biosciences AG... - Page 45 7) Position the black Sealing Ring (f) over the Ejector Sleeve (h) at the top of the Cylinder Assembly. Turn clockwise until the Sealing Ring snaps into place and secures the Ejector Sleeve in the Cylinder Assembly. 8) Slide the Cylinder Assembly over the Piston. www.integra-biosciences.com...
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Page 46: Assembly Of Multichannel Fixed Spacing Pipettes
Reassembly: Reengage the ball and socket to reconnect. Step 4 Disassembly: Remove the aluminum ring from the upper assembly. Reassembly: Slide the aluminum ring back onto the upper assembly. (The flanged surface goes into the upper assembly.) INTEGRA Biosciences AG... - Page 47 Remove the two screws from the rear housing. Reassembly: Replace the small screws from the rear housing. Step 9 Disassembly: Separate the housing from the cylinder/piston assembly. Reassembly: Place the rear housing (b) back onto the front housing/piston assembly (a). www.integra-biosciences.com...
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Page 48: Sterilization
Do not autoclave the entire unit. The extreme heat can damage the display and other electrical components. VOYAGER pipettes can NOT be autoclaved, they might be damaged! As-found calibration (measurement report, indicating “before” data) is not possible after autoclaving! INTEGRA Biosciences AG... -
Page 49: Autoclaving The Disassembled Components
You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes. After autoclaving send the pipette for service. Servicing 6.3.1 Shipping to INTEGRA Biosciences For any service or repairs, please contact your local service technician. ARNING If working with infectious materials, e. g. human pathogens, VIAFLO pipettes need to be decontaminated before sending them to service and the declaration on the absence of health hazards must be signed. -
Page 50: Lubrication
Single channel 1250 µl and 5000 µl: Use the Super-O-Lupe (#100-00135-50) to lightly lubricate the outer ring of the Cup Seals (d, e). For the 5000 µl pipette also lubricate the bottom of the white Cup Seal. INTEGRA Biosciences AG... -
Page 51: Calibration
Additionally, it equilibrates humidity of the air space inside tip and the pipette. Touch off: Touching the pipette tip against the surface liquid or side of well-plate to release any sample liquids that might be on the end of the pipettor tip. www.integra-biosciences.com... -
Page 52: Test Conditions And Environment
6. Aspirate by pressing the Run key. 4) Dispense: Keep the Run key pressed until all liquid is expelled, remove the tip from the liquid and then release the Run key to perform a two-step blowout, see “4.3.2 Blowout modes” on page 21. INTEGRA Biosciences AG... -
Page 53: Leak Test
4) Droplets are forming when holding the pipette in the air for 20 seconds, even if a pre- wet was performed. 5) The liquid levels are not equal on all channels after aspiration. If the pipette is leaking, change O-rings (see 6.3.2) and lubricate pistons (see 6.3.3) or contact your service technician. www.integra-biosciences.com... -
Page 54: Obtaining The Actual Volume
Maintenance 6.4.6 Obtaining the Actual Volume INTEGRA certifies the pipettes at 10%, 50% and 100% of the nominal value. For each volume 5 measurements are taken from all channels. General 1) Always use new, unused GripTips, also when changing the test volume (e.g. from 125 µl to 12.5 µl). -
Page 55: Calculation Of Accuracy And Precision
Add together the volumes i delivered, divide the sum by n (e.g. n = 5) to calculate the mean volume (in milliliters or micro liters) delivered at the test temperature, which is the Actual Volume: -- - www.integra-biosciences.com... -
Page 56: Adjusting Viaflo Electronic Pipettes
The “pipette” factor of the pipette’s software determines the performance of neat transfers and is therefore adjusted in case a pipette performs out of specifications. Adjusting a pipette in “Repeat Calibration Mode” is normally not required and is not described in this document. INTEGRA Biosciences AG... - Page 57 Select the Repeat factor in the calibration menu and enter the target and actual (measured) volume. b) Press calibrate. c) Then select Pipet factor and enter target and actual volume. d) Press calibrate. e) Both factors should be the same now. f ) Press Save. www.integra-biosciences.com...
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Page 58: Equipment Disposal
VIAFLO Pipettes – Operating instructions V05 Maintenance Equipment disposal The VIAFLO Electronic Pipettes must not be disposed of with unsorted municipal waste. Dispose of the VIAFLO Electronic Pipettes in accordance with the laws and regulations in your area governing disposal of devices. INTEGRA Biosciences AG... -
Page 59: Technical Data
21.38 53.79 215.69 832.93 2.56 25.83 65.00 260.63 1006.46 3.00 30.24 76.10 305.12 1178.29 3.97 40.00 100.65 403.55 1558.39 5.35 53.91 135.65 543.91 2100.43 6.83 68.89 173.33 695.00 2683.89 7.69 77.50 195.00 781.88 3019.38 8.79 88.57 222.86 893.57 3450.71 www.integra-biosciences.com... -
Page 60: Pipette Specifications
5.12 1.92 1.60 0.35 2.56 0.96 10.0– 4023 4.00 1.20 26.67 10.00 300.0 2.00 0.60 5.33 2.00 1.60 0.35 2.67 1.00 50.0– 4024 6.00 1.10 25.60 9.60 1250.0 2.40 0.50 5.12 1.92 1250 1.20 0.30 2.56 0.96 INTEGRA Biosciences AG... - Page 61 3.20 1.60 5.0– 4042 12.5 3.75 1.50 25.60 9.60 125.0 62.5 2.50 0.70 5.12 1.92 1.60 0.35 2.56 0.96 The multichannel and VOYAGER pipettes’ specifications represent the performance of all channels of the pipettes. Precision = Coefficient of Variation. www.integra-biosciences.com...
- Page 62 12.5 6.00 3.50 25.60 9.60 5.0– 4.5– 125.0 62.5 3.00 1.00 5.12 1.92 2.00 0.70 2.56 0.96 The multichannel and VOYAGER pipettes’ specifications represent the performance of all channels of the pipettes. Precision = Coefficient of Variation. INTEGRA Biosciences AG...
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Page 63: Z Correction Factors
1.0034 23.0 1.0033 1.0033 1.0034 1.0034 1.0035 1.0035 1.0036 23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0036 1.0037 24.0 1.0035 1.0036 1.0036 1.0037 1.0037 1.0038 1.0038 24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039 1.0039 Z values in microliters per milligram www.integra-biosciences.com... -
Page 64: Chapter 8 Accessories
12.5 µl, 125 µl and 300 µl pipettes Reagent Reservoirs Part No. 10 ml Disposable reagent reservoirs, sterile, individually sealed, 4331 30 reservoirs per case Disposable reagent reservoirs, sterile, four sleeves of 4332 50 reservoirs per case Reservoir Base, 10 pack 4306 INTEGRA Biosciences AG... - Page 65 5 inserts of 384 tips, non-sterile, GREEN CHOICE 4422 5 boxes of 384 tips, non-sterile 4423 5 boxes of 384 tips, sterile 4424 5 boxes of 384 tips, sterile, filter 4425 5 inserts of 384 tips, pre-sterilized, GREEN CHOICE 4426 www.integra-biosciences.com...
- Page 66 4444 5 boxes of 96 Tips, sterile, filter 4445 5 inserts of 96 tips, pre-sterilized, GREEN CHOICE 4446 5 ml 1 bag of 250 tips, non-sterile, bulk pack 4451 Individually wrapped, 100 tips per case, sterile 4456 INTEGRA Biosciences AG...
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