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CELLROLL and CELLSPIN Operating instructions 183411_V12...
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Declaration of Conformity | Konformitäts- erklärung | Déclaration de conformité INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the products | erklärt in alleiniger Verantwortung, dass die Produkte | déclare sous sa responsabilité exclusive, que les produits in accordance with the EC directives | gemäß...
Danger: Warning that, if safety precautions are not observed, injury to personnel may result. Installation: Location and conditions CELLROLL/CELLSPIN are intended for use in a laboratory. Ambient conditions for the devices CELLROLL/ CELLSPIN see chapter Technical data Warning: Always locate the control unit outside of an incu- bator if used.
Conditions of use CELLROLL is a modular and expandable roller system for cell cul- tivation and preparation of cell products in roller bottles. It is suit- able for use with adherent cells and cell suspensions. The instru- ment is not designed for any other applications and should there- fore not be used for such.
INTEGRA Biosciences parts. CELLROLL/CELLSPIN may not be altered in design or in any of their safety aspects without the express written permission of INTEGRA Biosciences. In particular, no alterations may be made to any of the protective functions.
3. Abridged operating instructions CELLROLL /CELLSPIN 3.1 Instrument parameters CELLROLL CELLROLL CELLSPIN Motor unit fast (2 – 6 rpm) Motor unit (0.1 – 2 rpm) "SPEED" for continuous rolling "SPEED" for continuous rolling "SPEED" for continuous stirring (rolling with no directional change)
3.2 Brief description of keys and functions Keys: ON/OFF: Switches the instrument on/off SELECT: Switches between parameters. The corresponding green LED will light and the most recently set value will appear in the display. ENTER: For confirming the value entered. Keys "∨"...
3.3 Abridged operating instructions - CELLROLL NB: The control unit must always be located outside the incubator! – Connect CELLROLL to the control unit with the cable provided. If connection has not been made, "Err 02" will appear on the display on starting the instrument.
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In the function " – ": The pause interval cannot be selected for either motor unit and is overrun. In the function "CYCLES": Repeating programs: Programs can be set to repeat within the range 1 - 9999. Select the required value via "∨" and "∧". Confirm by pressing "ENTER".
3.4 Abridged operating instructions - CELLSPIN NB: The control unit must always be located outside the incubator! – Connect CELLSPIN to the control unit with the cable provided. If connection has not been made, "Err 02" will appear on the display on starting the instrument.
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In the function " – ": Setting the interval: Within any program, an interval can be freely selected within the range 00.00 (h/min) - 99.59 (h/min). Choose the position for hours and minutes via key "< >" and set the interval via keys "∨" and "∧". Confirm by pressing "ENTER".
Connection for power cable and integrated interface RS 232 C for connecting a printer/PC (activated via Y-cable) to POWER IN. Two asynchronous outputs - OUTPUT 1 and OUTPUT 2 for connecting: – 1 CELLSPIN and 1 CELLROLL – or 2 CELLSPINS or 2 CELLROLLS...
4.4 Designation of individual instrument parts CELLROLL - Motor unit x Socket Deck CELLSPIN -stirring unit with 4 stirrers z Left cable channel { Socket | Right cable channel Control cable } Socket ~ Plug Mains cable Socket...
The top deck of a tower remains without connectors. The gears of the decks mesh together automatically. Locate CELLROLL in such a way that personnel do not come in contact with the cogs. NB: Never stack more than 4 decks at any one time.
The top deck or each tower remains without intermediaries. Continue assembly following the instructions. Place the CELLROLL motor unit with its ex-factory fitted con- nectors on the top deck of one of the towers and ensure that it engages in position.
Connect the power pack to the source of electricity. NB: Always locate the control unit outside of the incu- bator. 4.7 Disassembly - CELLROLL Disconnect the power cable. Remove the low voltage cable from the clips. Disconnect the plug of the low voltage cable from control unit.
(temperature, humidity etc.) are not necessary, the control unit can be fitted directly to the guide slot on the motor unit. Ensure that it engages. NB: In setting up CELLROLL/CELLSPIN, please observe the conditions for location and setting up as outlined in section 1.
Data bits: Start bit: Stop bit: 4.10 Equipment disposal The CELLROLL and CELLSPIN motor units and the control unit are labelled with the "crossed-out wheeled bin" symbol to indicate that this equipment must not be disposed of with unsorted municipal waste.
5. Detailed Operating Instructions Please read and observe the safety precautions listed on page 5. 1. If, during entry of a new program or alteration of individual parameters a pause >120 sec arises, the current speed will appear in the display. 2.
– CS for CELLSPIN – Cr for CELLROLL with "motor unit" 0.1 to 2.0 rpm – CrF for CELLROLL with "motor unit fast": 2.0 to 6.0 rpm 5.2 CELLSPIN – Instrument-specific functions Stirring speed - "SPEED"...
Setting the angle for pendular stirring In the function " " the angle for pendular stirring can be set. Pendular Angle „SPEED“ rpm stirring 180°–1440° 5.0 – 40.0 rpm in steps of 2.5 * If values > 40 rpm are entered, the angle " "will automatically be overrun.
In the function "––", the time interval between repetition of the pro- gram can be set. For both motor units of CELLROLL the function pause can not be selected and will be automatically overrun. The interval between program runs can be set for CELLSPIN with- in the range 00.00 h.min to 99.59 h.min.
Setting the speed for continuous rolling and rocking: Press the "SELECT" key until the green light under "SPEED" and "rpm" lights. The currently set and speed will appear in the display. Set the required speed using the "∨" or "∧" keys. The display will blink.
5.4 CELLROLL/CELLSPIN - common functions Running time mode " " The running time is the duration of stirring or rolling and can be set from: - 00.05 h.min to 99.59 h.min or - unlimited = Con In the mode "...
Cycle mode "CYCLES" In the "CYCLES" mode, the number of cycles can be set from 1 to 9999. One cycle is the combination of the set running time and the set interval (see CELLSPIN). N.B.: If continuous running time has been entered, "Con" will appe- ar in the display automatically in the mode CYCLES.
PRINT - print-out and overview of program parameters Options of the function PRINT: - Viewing stored programs - P1 - Print-out of protocols- P2 - Print-out of stored programs - P3 Selecting options: Press the "SELECT" key until the green light under "PRINT" lights.
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In this option, all stored programs can be printed out. For each of the system components, i.e. • CELLSPIN • CELLROLL "Motor unit" • CELLROLL "Motor unit fast" • External device there are 10 memories available for each; these are noted on the print-out.
Storing programs - "STORE" mode For each of the connected system components, 10 memories (Pr. 01 to 10) are available for program storing. N.B.: If two identical system components (e.g. two Cellrolls, two Cell- spins) have been connected, each will be allocated 5 memories. In addition, one further memory is provided with the following fun- ction: In the case of power failure, all data of the current program for both outputs which have not been stored under "STORE"...
6. Troubleshooting Problem Probable cause Remedial measure CELLROLL or CELLSPIN control • Power interruption • Check all connections between control unit doesn't switch on by unit, power supply and wall socket. pressing the "ON/OFF" key • Faulty power supply • Replace the power supply with a new one.
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INTEGRA Biosciences AG. This operating instruction manual has the article number 183 411, version number V12.
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