Integra VIAFLO 96 Operating Instructions Manual
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Integra VIAFLO 96 Operating Instructions Manual

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VIAFLO 96/384 Operating instructions
125950_V11

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Summary of Contents for Integra VIAFLO 96

  • Page 1 VIAFLO 96/384 Operating instructions 125950_V11...
  • Page 2 Declaration of conformity INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the devices Description Models VIAFLO 96 6000, 6001 VIAFLO 384 6030, 6031 comply with: EU Directives (DoW: Date of Withdrawal) Before DoW After DoW...

  • Page 3: Table Of Contents

    Installation 3.1 Operating environment ..............12 3.2 Setting up and moving of the instrument ........12 3.3 Getting started ................13 3.4 Toolbox - adapt your VIAFLO 96/384..........14 3.4.1 Change head ..............14 3.4.2 Storage of pipetting heads ..........15 3.4.3 Position Settings ..............16 3.4.4 Preferences.................16 3.4.5 Calibration &...
  • Page 4 VIAFLO 96/384 – Operating instructions V11 Chapter 4 Operation 4.1 Turn on/off the device..............21 4.2 Attaching and removing GripTips ...........21 4.2.1 Loading tips from a complete box ........21 4.2.2 Partial tip loading ..............22 4.2.3 Ejecting used GripTips............22 4.3 Start pipetting .................23 4.3.1 Pipetting ................23...
  • Page 5 VIAFLO 96/384 – Operating instructions V11 Chapter 6 Maintenance 6.1 Cleaning and Decontamination ............47 6.1.1 Base unit ................47 6.1.2 Pipetting heads ..............47 6.2 Prolonged period of non-use ............47 6.3 Leak test ..................48 6.4 Servicing..................49 6.4.1 Blocked pipetting unit............49 6.4.2 Changing O-rings of tip fittings..........50 6.5 Calibration ..................51...
  • Page 6 © 2017 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.

  • Page 7: Chapter 1 Introduction

    0.5 µl to 1250 µl using GripTip pipette tips. VIAFLO 96/384 is used like a hand held pipette. The movement and positioning of the 96-/384-channel pipette is supported by a servo assisted steering mechanism which allows fast, precise and stress free multichannel pipetting.

  • Page 8: Safety Notes

    CR (chloroprene), silicone. Corrosive vapors could also damage metallic parts inside the device. Do not open or modify VIAFLO 96/384 in any way. The sheet metal must not be removed. Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after-sales service member.

  • Page 9: Description Of The Device

    VIAFLO 96/384 – Operating instructions V11 Description of the device Description of the device Scope of delivery • VIAFLO 96/384 device • Power cable • USB cable type A to B • Operating instructions AUTION Verify the scope of delivery when unpacking the device and check for potential transportation damage.

  • Page 10: Viaflo 96/384 Control Unit

    VIAFLO 96/384 – Operating instructions V11 Description of the device 2.2.2 VIAFLO 96/384 control unit 11 Display 12 Back button, to navigate backward 13 Touch wheel, spin to scroll and move the cursor 14 OK button, to make a selection...

  • Page 11: Touch Wheel

    VIAFLO 96/384 – Operating instructions V11 Description of the device 2.2.4 Touch wheel The Touch wheel is fully operational with only one hand. Rotational finger movements translate into up and down cursor movement on the display. The Touch wheel is fully functional with the use of latex gloves.

  • Page 12: Chapter 3 Installation

    Installation Installation Operating environment VIAFLO 96/384 has been designed for use in a laboratory. It shall be operated in a dry and dust-free location with a temperature of 5–40 °C and a maximal (non-condensing) relative humidity of 80 %. Setting up and moving of the instrument The VIAFLO 96/384 must be set up on a cleaned, dry and horizontal surface.

  • Page 13: Getting Started

    Switch off VIAFLO 96/384 and disconnect it from the electricity mains. Getting started Turn on VIAFLO 96/384 (see "Turn on/off the device" on page 21). To adapt the device to the appropriate applications, select the following functions of the Toolbox menu in this order: •...

  • Page 14: Toolbox - Adapt Your Viaflo 96/384

    The Help information describes the modes and some settings, press  to select the Help option. 3.4.1 Change head VIAFLO 96 can only be used with 96-channel pipetting heads, while VIAFLO 384 is compatible with both 96- and 384-channel pipetting heads. The following pipetting heads are available: Pipetting head...

  • Page 15: Storage Of Pipetting Heads

    3.4.2 Storage of pipetting heads Once a pipetting head is removed from VIAFLO 96/384 it must be stored in the appropriate casing to protect it from dust and mechanical damage. AUTION Pipetting heads with 384-channels must always be stored in extended position.

  • Page 16: Position Settings

    VIAFLO 96/384 – Operating instructions V11 Installation 3.4.3 Position Settings Position settings contain options that help to find correct pipetting positions. These settings are globally stored and are valid for all pipetting heads. Perform these alignments with GripTips attached, see “4.2 Attaching and removing GripTips” on page 21.
  • Page 17 VIAFLO 96/384 – Operating instructions V11 Installation Handle Sen- Sets the sensitivity of the control unit to move the sitivity pipetting unit and base unit. • Use the Touch wheel to set a sensitivity 1 (low) to 9 (high). Press OK.

  • Page 18: Calibration & Services

    Description Range & Services Calibration Allows for re-calibration of VIAFLO 96/384 to restore accuracy. The calibration factors for Pipet and Repeat type are displayed. To edit the calibration volumes, press . • Target Volume: This is the volume you are interested in using for the calibration.

  • Page 19: Communications

    VIAFLO 96/384 – Operating instructions V11 Installation 3.4.6 Communications VIAFLO 96/384 can be programmed from a PC via USB communication cable (type A to Communi- Description cations VIALINK Connect the USB cable betweenVIAFLO 96/384 and a PC. Press OK to begin bi-directional communication.

  • Page 20: Write Protect

    VIAFLO 96/384 – Operating instructions V11 Installation 3.4.9 Write protect Select this option to protect programs and menu options from inadvertent modification. The pipetting programs can still be used. Write Description Range protect Select an option and press OK to switch protection on or /...

  • Page 21: Chapter 4 Operation

    Remove hands from Control unit (5) at switch on and during homing. When VIAFLO 96/384 is turned on by the Main switch (10), you are prompted to press the RUN key (17) to perform a vertical and horizontal homing routine.

  • Page 22: Partial Tip Loading

    VIAFLO 96/384 – Operating instructions V11 Operation When prompted push the Tip load button (a) and at the same time push down the Control unit (b). When the tips are loaded, the Tip load button lights up. Move the Pipetting unit (3) up until the light of the Tip load button turns off.

  • Page 23: Start Pipetting

    VIAFLO 96/384 – Operating instructions V11 Operation Start pipetting 4.3.1 Pipetting Use the Touch wheel (13) to scroll to your desired pipetting mode and press OK (14). Actions you are about to perform will be displayed on the Run screen.

  • Page 24: Recommendations For Pipetting

    • Calibrate based on fluid type. VIAFLO 96/384 is tested and calibrated at the factory for use with distilled water at room temperature. It may be necessary to re-calibrate your VIAFLO 96/384 if the liquid to be used has different physical properties (specific gravity and vapor pressure) than water.

  • Page 25: Pipetting Options And Settings

    VIAFLO 96/384 – Operating instructions V11 Operation Pipetting options and settings 4.4.1 Edit option The Edit option is available for each mode. It enables you to access the variables that you can adjust for a pipetting mode. These variables include Speed, Volume, Pace, Count, Mix Cycles, Rows and Direction.

  • Page 26: Speed Selection

    VIAFLO 96/384 – Operating instructions V11 Operation Define and select favorite volumes You can define, save, and select up to ten favorite volumes for quick access. These volumes can only be within the pipetting head volume range. There are two ways to access and customize the list of favorite volumes: •...

  • Page 27: Custom

    VIAFLO 96/384 – Operating instructions V11 Operation 4.4.4 Custom You can convert any predefined pipetting mode into a Custom program. After setting up a pipetting mode with your parameters, use the Touch wheel to select  Custom. You are prompted to enter an name. Use the Touch Wheel to select characters and press OK.

  • Page 28: Pipetting Between 96 And 384 Well Plates

    VIAFLO 96/384 – Operating instructions V11 Operation 4.4.7 Pipetting between 96 and 384 well plates For fast and simple reformatting between 96 and 384 well microplates and for loading of 12.5 or 125 µl GripTips with a 96-channel pipetting head, some Plate holders feature a slide function to shift the microplate in Y-direction beneath the pipetting head.

  • Page 29: Pipetting With The Three Position Stage

    VIAFLO 96/384 – Operating instructions V11 Operation 4.4.8 Pipetting with the three position stage The three position stage (see “8.1 Accessories” on page 55) supports the pipetting on two of three possible positions. Move the three position stage to the right to pipette on position A and AB.

  • Page 30: Troubleshooting/Faq

    VIAFLO 96/384 – Operating instructions V11 Operation Troubleshooting/FAQ Problem Probable cause Remedy Base unit is drifting Control unit was touched at Do not touch the control unit when sidewards. switch on. switching on the VIAFLO 96/384. Restart VIAFLO 96/384 to initiate new homing routine.

  • Page 31: Pipetting Modes

    Pipetting modes Pipetting modes This chapter describes how to program the VIAFLO 96/384 in two ways: • Function-based pipetting modes: You can select from ten predefined pipetting modes that you can quickly and easily edit and execute. They are described in the following sections.

  • Page 32: Detailed Description Of Pipetting Modes

    Pipetting modes Detailed description of pipetting modes VIAFLO 96/384 offers ten predefined pipetting modes. Most liquid handling protocols can be easily accommodated using one or more of these modes. The options and steps of the different pipetting modes are described in the following subsections.

  • Page 33: Repeat Dispense Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.2 Repeat dispense mode Application: This mode can be used for fast reagent addition to microplates from one source container. You can dispense a large aspirated volume of liquid in multiple aliquots to multiple targets.

  • Page 34: Sample Dilute Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.3 Sample dilute mode Application: Accomplish accurate sample dilutions by using diluent to “chase” small sample volumes from the pipet tips. An air gap keeps liquid separated in the tips and helps to minimize carryover of diluent when aspirating the sample.

  • Page 35: Manual Pipet Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.5 Manual pipet mode Application: This mode can be used when the aspiration volume is not defined or unknown. You have control over the aspiration and dispense steps and can view the display to confirm how much liquid has been aspirated or dispensed.

  • Page 36: Reverse Pipet Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.6 Reverse pipet mode Application: With this mode the aspiration volume is higher than the volume dispensed. It is recommended for liquid transfers of viscous and high vapor pressure liquids. The dispense method prevents introduction of air into the sample because no blowout is made.

  • Page 37: Variable Dispense Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.7 Variable dispense mode Application: Use this mode when differing dispense volumes are required. This mode could be used to quickly set up a dilution series in plates or for feeding similar samples to different assay plates where different sample volumes are needed.

  • Page 38: Variable Aspirate Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.8 Variable aspirate mode Application: This mode can be used for a variety of collection applications where the aspiration volume is well known. This mode is also suited for supernatant collection in microplates.

  • Page 39: Sample Dilute/Mix Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.9 Sample dilute/mix mode Application: Use this mode to perform sample dilutions where mixing of sample and diluent is required. This mode could also be used to introduce and mix diluent and sample to the first column of a serial dilution plate.

  • Page 40: 5.2.10 Serial Dilution Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.2.10 Serial dilution mode Application: Use this mode to perform serial dilutions. The Serial Dilution mode enables aspiration of a specific volume followed by a mix sequence and ending with the original aspiration volume in the tips.

  • Page 41: Custom Step-Based Programming Mode

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes Custom step-based programming mode Application: Use the Custom program mode to create personalized pipetting protocols. Up to forty programs can be stored. Select “Custom” to create a personalized protocol. Programs can contain up to 98 individual steps based upon the following basic operations: Aspirate, Dispense, Mix, Purge, Prompt, Set Z Height, Tip Align and Loop.

  • Page 42: Manual Custom Program

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.3.1 Manual custom program Manual custom programs can consist of the following steps: Step Description Aspirate Sets an aspiration volume and speed. Dispense Sets a dispense volume and speed. Sets the mixing volume after dispensing.
  • Page 43 VIAFLO 96/384 – Operating instructions V11 Pipetting modes Example of manual custom program The task is to combine 2 different liquids in a 96 well plate for a kinetic assay and then mixing it to achieve a homogeneous solution. The microplate should then be incubated for 5 minutes and the content is then distributed to a 384 well plate.

  • Page 44: Modify Existing Programs

    VIAFLO 96/384 – Operating instructions V11 Pipetting modes 5.3.2 Modify existing programs At the Custom program display, use the Touch wheel to highlight an existing program. Press  Options, use the Touch wheel to select an option (View/Edit, Delete, Copy, Rename) to modify the program and press OK Press Back to return to the list of Custom programs.
  • Page 45 The program continues with the next step after pressing the RUN key. As manual custom programs, automatic programs can be created directly on VIAFLO 96/ 384. However, we recommend using the VIALINK software to set up automatic custom programs, see “5.4 VIALINK” on page 46.

  • Page 46: Vialink

    GripTips cannot be attached automatically. VIALINK VIALINK is a pipette management software for the PC and is used to manage VIAFLO 96/ 384 and VIAFLO electronic hand held pipettes. It allows to create custom programs, manage a library of custom programs, establish a service history and more.

  • Page 47: Chapter 6 Maintenance

    Prolonged period of non-use Pipetting heads of VIAFLO 96/384 should not be left inside the device if not used for extended periods (>1 week). Remove the pipetting head from the device and store it wrapped in a plastic bag in the appropriate casing.

  • Page 48: Leak Test

    VIAFLO 96/384 – Operating instructions V11 Maintenance Leak test It is recommended to perform a leak test every 3 months or when errors occur. Leak test may be done on half of the head at a time or column by column for a better view of the liquid levels (load one GripTip column with reduced force as described under operation of “4.2.2 Partial tip loading”...

  • Page 49: Servicing

    If the control unit is defect or the pipetting unit parks in down position and the pipetting head can not be removed, start the following procedure: 1) Turn off the VIAFLO 96/384. Turn it back on while pressing the Tip load button (4) for approx. 5 sec. until the Tip load button lights up permanently.

  • Page 50: Changing O-Rings Of Tip Fittings

    VIAFLO 96/384 – Operating instructions V11 Maintenance 6.4.2 Changing O-rings of tip fittings 300 µl and 1250 µl pipetting heads feature tip fittings with red O-rings to seal against the inside wall of GripTips providing optimal sealing. O-rings are made of durable silicone.

  • Page 51: Calibration

    Maintenance Calibration VIAFLO 96/384 can be calibrated by adjusting a correction factor in the software. The correction factor is determined by a nominal volume and the measured volume. To assess the accuracy and precision of VIAFLO 96/384 pipetting heads, different methods can be used: •...

  • Page 52: Chapter 7 Technical Data

    VIAFLO 96/384 – Operating instructions V11 Technical Data Technical Data Environmental conditions Operation Temperature range 5–40°C Humidity range Max. rel. humidity 80 % for temperatures up to 31 °C, decreasing linearly to 50 % rel. humidity at 40 °C. Altitude range <...

  • Page 53: Intellectual Property

    VIAFLO 96/384 – Operating instructions V11 Technical Data Intellectual Property The VIAFLO 96/384 are covered under the following patents: Patent Number Country Title Apply to 8,033,188 Pipettor Software Interface VIAFLO 96/384 2192985 EPC/ Pipettor Software Interface VIAFLO 96/384 FRAN/ GBRI/...

  • Page 54: Pipetting Specifications

    VIAFLO 96/384 – Operating instructions V11 Technical Data Pipetting specifications Volume Min. Scrolling Test Volume Accuracy Precision Head Range Volume Part No. (µl) Increments (µl) (µl) (±%) (≤%) 1.25 6101 0.5–12.5 0.01 6.25 6131 12.5 (1 µl–) 6106 2–50 0.05 6136 12.5...

  • Page 55: Chapter 8 Accessories And Consumables

    VIAFLO 96/384 – Operating instructions V11 Accessories and consumables Accessories and consumables Accessories Pipetting heads Volume range Part No. 96-channel 0.5–12.5 µl 6101 pipetting heads 2–50 µl 6106 5–125 µl 6102 10–300 µl 6103 50–1250 µl 6104 384-channel 0.5–12.5 µl...

  • Page 56: Consumables

    VIAFLO 96/384 – Operating instructions V11 Accessories and consumables Consumables GripTips for VIAFLO 96/384 Part No. Purple: 5 XYZ racks of 384 tips, non-sterile, LONG 6403 12.5 µl GripTips 5 XYZ racks of 384 tips, sterile, LONG 6404 5 XYZ racks of 384 tips, sterile, filter, LONG...
  • Page 57 VIAFLO 96/384 – Operating instructions V11 Accessories and consumables Automation friendly disposable reagent reservoirs Part No. 150 ml 8 reservoir bases, automation friendly (microplate for- 6301 mat), non-sterile 25 lids, sterile, polypropylene 6302 Trial pack (1 base, 1 reservoir and 1 lid), sterile...

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