Integra 4500 Operating Instructions Manual
Hide thumbs
Also See for 4500:
- Operating instructions manual (29 pages) ,
- Operating instructions manual (35 pages)
Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Subscribe to Our Youtube Channel
Related Manuals for Integra 4500
Summary of Contents for Integra 4500
- Page 1 VIAFLO ASSIST Operating instructions 127950_V07...
- Page 2 Declaration of conformity INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the device Description Model VIAFLO ASSIST 4500 complies with: EU Directives (DoW: Date of Withdrawal) Before DoW After DoW Low Voltage Equipment 2006/95/EC 20.04.2016...
-
Page 3: Table Of Contents
3.6.5 Move Speed................13 Chapter 4 Operation 4.1 Turn on/off the VIAFLO ASSIST device .........14 4.2 Turn on/off the VIAFLO II Electronic Pipette ........14 4.3 Bluetooth connection..............14 4.4 Running a program.................14 4.5 Pipetting in 96 and 384 well plates ..........15 4.6 Troubleshooting................16 www.integra-biosciences.com... - Page 4 7.2 Specification of the device..............30 7.2.1 Maximum labware height on plate carrier ......30 7.3 Intellectual Property................30 Chapter 8 Accessories 8.1 Accessories for VIAFLO ASSIST ...........31 8.2 Accessories for VIAFLO II Electronic Pipettes .......31 8.3 Consumables .................32 Imprint ....................35 INTEGRA Biosciences AG...
-
Page 5: Chapter 1 Introduction
This symbol identifies important notes regarding the correct operation of the device and labor-saving features. The device is marked with the following symbol: IOHAZARD The instrument can be potentially biohazardous due to the use of biozazardous substances by the operator. www.integra-biosciences.com... -
Page 6: Intended Use
They are used for aspirating and dispensing liquids in the volume range of 0.5– 1250 µl using GripTip pipette tips. Please refer to the VIAFLO II Electronic Pipettes operating instructions for more detailed informations on www.integra-biosciences.com. INTEGRA Biosciences AG... -
Page 7: Safety Notes
Regardless of the listed safety notes, additional applicable regulations and guidelines of trade associations, health authorities, trade supervisory offices, etc. must be observed. Please visit our website www.integra-biosciences.com on a regular basis for up to date information regarding REACH classified chemicals contained in our products. -
Page 8: Description Of The Device
Green LED blinks: press to start operation/homing Green LED lights: operation performed 14 Bluetooth control LED (lights blue if connection is active) and Error LED (flashes red in case of error) 15 On/Off key (lights green if On) INTEGRA Biosciences AG... -
Page 9: Overview Of Viaflo Ii Electronic Pipette
24 Finger hook, facilitates easy operation 25 Volume indicator label, color matches GripTip box insert. 2.3.2 Display The Display shows all pipetting options. Battery life indicator OK - Serial Dilution, select program Current mode Instruction Active step Volume Options www.integra-biosciences.com... -
Page 10: Chapter 3 Installation
When it turns red, the pipette needs to be charged. AUTION Use only the approved INTEGRA battery, power supply or charging stand. Use of an incompatible power transformer can damage the pipette. The battery can be charged using either the mains adapter or a charging stand, see (“8.2 Accessories for VIAFLO II Electronic Pipettes”... -
Page 11: Attaching And Removing A Viaflo Ii Electronic Pipette
From the ASSIST option select Bluetooth Pairing. Switch OFF and ON the VIAFLO ASSIST device, see “4.1 Turn on/off the VIAFLO ASSIST device” on page 14, and wait approx. 30 sec. until the message “Pairing successful” is displayed. Press OK. www.integra-biosciences.com... -
Page 12: Setting Tip Type
For fine adjustment of the pipette tips along the Y-axis turn the screw on the pipette holder (a) with a flathead screw driver size 2. Turning clockwise moves the pipette to the rear, counterclockwise to the front. The maximum Y-shift is +/- 5 mm. INTEGRA Biosciences AG... -
Page 13: Move Speed
Allows to change the move speed of the pipette arm (1, Z-axis) and the plate carrier (2, X- axis) on ASSIST. Select Move Speed and press OK. Use the Touch wheel to select Slow, Medium or Default (=fast) and press OK. Save your settings. www.integra-biosciences.com... -
Page 14: Chapter 4 Operation
It is possible to create programs, however without teaching of active heights. Running a program From the ASSIST menu select the Pipetting mode and the stored program you want to run and press OK. Press the Run key (22). INTEGRA Biosciences AG... -
Page 15: Pipetting In 96 And 384 Well Plates
(a) moves the pipette center to pipette in 96 well lever (a) moves the pipette to the rear to accommo- plates. to the front to accommo- date the rear positions of date the front positions of 384 well plates. 384 well plates. www.integra-biosciences.com... -
Page 16: Troubleshooting
“3.6.4 Adjusting the plate tion. alignment” on page 12. The tip height is not • Wrong tip type set. • Enter the tip type used, see correct using 12.5 μl “3.6.3 Setting tip type” on GripTips. page 12. INTEGRA Biosciences AG... -
Page 17: Chapter 5 Programming
Variable Dispense Allows dispensing multiple aliquots of different volumes. Multi Aspirate Allows aspirating multiple aliquots of the same or different volumes from a microplate. Custom Allows to create and store multi-stepped user-defined pipetting protocols. www.integra-biosciences.com... -
Page 18: Setting Up A Program
Modify existing programs At any program display, use the Touch wheel to highlight an existing program. Press Options, use the Touch wheel to select an option (View/Edit, Delete, Copy, Rename) to modify the program and press OK. INTEGRA Biosciences AG... -
Page 19: Detailed Description Of Pipetting Modes
Note: Switching to portrait requires to adapt default parameters like “Rows”, “Columns” or “Count”. Clear Height The travel height at which the GripTips move from well to well. The tips must be above the plate, see “Adjust the tip heights” on page 20. www.integra-biosciences.com... - Page 20 Start tracking from (h Start tracking from (x): Start tracking from (x): Height (h ) is defined in Height (x) is calculated Height (x) is calculated the Target section. automatically: (h automatically: (h Target Disp distance. Target Disp distance. INTEGRA Biosciences AG...
-
Page 21: Serial Dilution Mode
“Description of the tracking distances” on page 20. Set the mixing volume after dispensing. It does not affect the transfer volume. Mix Speed Set the mixing speed (1 = low, 10 = fast). Mix Cycles Set the number of mixes per well (1-30). www.integra-biosciences.com... - Page 22 The same distance is used to move down during aspiration in the target location. • Target Mix: moves the set distance down and up during mixing in the target location. Press to save your settings. This will return you to the list of Serial Dilution programs. INTEGRA Biosciences AG...
-
Page 23: Repeat Dispense Mode
Select the INTEGRA reagent reservoir for multichannel pipettes, see 8.3 type “Consumables” on page 32. Start volume Set the filling volume that is currently in the INTEGRA reagent reservoir. Set the approx. immersion depth of the pipette tips (2-3 mm are immersion recommended). - Page 24 Dispense and the blowout height (BlowOut at, only activated if Tracking is disabled). Set the Blowout height below the liquid in the wells for best results. Press to save your settings. This will return you to the list of Repeat Dispense programs. INTEGRA Biosciences AG...
-
Page 25: Variable Dispense Mode
Dispense Option Description Dispense Set the different dispense volumes for every variable dispense step. 1 to n Press to save your settings. This will return you to the list of Variable Dispense programs. www.integra-biosciences.com... -
Page 26: Multi Aspirate Mode
• Source Asp: set the distance in mm, which the pipette should move down (or up) during aspirating (and mixing) from the source plate, Press to save your settings. This will return you to the list of Multi Aspirate programs. INTEGRA Biosciences AG... -
Page 27: Custom Program Mode
The distance from well to well for a 96 well plate is 9 mm and for a 384 well plate 4.5 mm. 1. Tracking distance: the distance the pipette automatically follows during aspirating, dispensing or mixing allowing a constant tip immersion depth, see “Description of the tracking distances” on page 20. www.integra-biosciences.com... - Page 28 Beep Sets a beep. The sound is only active, if under Preferences - Sounds the option Messages is set to On. When finished, press to save the Custom program. To run the program, press OK. INTEGRA Biosciences AG...
-
Page 29: Chapter 6 Maintenance
This is necessary to protect service personnel. Equipment disposal The VIAFLO ASSIST must not be disposed of with unsorted municipal waste. Dispose the VIAFLO ASSIST in accordance with the laws and regulations in your area governing disposal of devices. www.integra-biosciences.com... -
Page 30: Technical Data
50 µl / 125 µl 101.3 mm 300 µl 96.0 mm 1250 µl 54.1 mm Intellectual Property The VIAFLO ASSIST is covered under the following patents: Patent Number Country Title Apply to 9,321,048 Sample Distribution System And Pro- ASSIST cess INTEGRA Biosciences AG... -
Page 31: Chapter 8 Accessories
Mains Adapter for linear stand and carousel charging stand 3216 Charging station for linear stand, incl. connection cable 3217 Charging/communication station for linear stand, incl. connection cable 3218 and USB cable Bluetooth module for VIAFLO II pipettes 4221 Bluetooth PC module with PC software 4225 www.integra-biosciences.com... -
Page 32: Consumables
Reservoir Base, 10 pack 4304 100 ml Disposable reagent reservoirs, individually sealed, 4321 30 reservoirs per case, sterile Disposable reagent reservoirs, four sleeves of 50 reservoirs, 4322 200 reservoirs per case, sterile Reservoir Base, 10 pack 4305 INTEGRA Biosciences AG... - Page 33 VIAFLO ASSIST – Operating instructions V07 Accessories GripTips for all INTEGRA Pipettes Part No. Purple 5 XYZ racks of 384 tips, sterile, filter, SHORT 6475 SHORT: 12.5 μl Purple Bulk pack, 1 bag of 1000 tips, non-sterile, LONG 4401 LONG:...
- Page 34 VIAFLO ASSIST – Operating instructions V07 Accessories GripTips for all INTEGRA Pipettes Part No. Green: Bulk pack, 1 bag of 1000 tips, non-sterile 4431 300 μl 5 inserts of 96 tips, non-sterile, GREEN CHOICE 4432 5 racks of 96 tips, non-sterile...
-
Page 35: Imprint
© 2017 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
Need help?
Do you have a question about the 4500 and is the answer not in the manual?
Questions and answers