Integra ASSIST PLUS Operating Instructions Manual
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Integra ASSIST PLUS Operating Instructions Manual

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ASSIST PLUS Operating instructions
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Summary of Contents for Integra ASSIST PLUS

  • Page 1 ASSIST PLUS Operating instructions 128950_V02...
  • Page 2 Declaration of conformity INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the ASSIST PLUS (Model 4505) complies with: EU Directives Low Voltage Equipment 2014/35/EU Electromagnetic Compatibility 2014/30/EU Restriction of Hazardous Substances 2011/65/EU Waste Electrical and Electronic Equipment...

  • Page 3: Table Of Contents

    3.9.6 Setting tip type ..............15 3.9.7 Position adjustment.............16 Chapter 4 Operation 4.1 Turn on/off the ASSIST PLUS device ..........17 4.2 Turn on/off an INTEGRA electronic pipette ........17 4.3 Connection ASSIST PLUS to Pipette ..........17 4.3.1 Bluetooth connection ............17 4.3.2 Via communication cable ............18 4.4 Running a program.................18...
  • Page 4 5.2 Setting up a program directly on the pipette........21 5.3 Modify existing programs ...............21 5.4 Adjust heights and positions............22 5.4.1 Enter heights/positions directly ...........22 5.4.2 Teach heights/positions on ASSIST PLUS ......22 5.5 Program offset ................23 5.6 Pipetting alternating wells...............24 5.7 Detailed description of predefined programs........25 5.7.1 Serial Dilution program ............25...

  • Page 5: Chapter 1 Introduction

    Introduction These operating instructions contain all the information required for installation, operation and maintenance of the ASSIST PLUS. This chapter informs about the symbols used in these operating instructions, the intended use of the ASSIST PLUS and the general safety instructions.

  • Page 6: Intended Use

    This product may not be directly exposed to the internet. If the ASSIST PLUS is used in a manner not specified by INTEGRA Biosciences, the protection provided by the ASSIST PLUS may be impaired.With a VIAFLO electronic multichannel or VOYAGER pipette attached, ASSIST PLUS performs pipetting operations automatically (for pipette compatibility see 7.4).

  • Page 7: Safety Notes

    AUTION Do not open or modify the ASSIST PLUS in any way. Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after-sales service member. Parts may be replaced with original INTEGRA Biosciences parts only.

  • Page 8: Chapter 2 Description Of The Device

    Description of the device Description of the device Scope of delivery • ASSIST PLUS (pipettes to be ordered separately, see “8.1 Accessories” on page 48) • Mains adapter • Tip waste bags sample pack • Trial pack 10 ml, 25 ml and 100 ml multichannel reagent reservoir •...

  • Page 9: Touch Panel

    Start/Pause key or follow (18 at the the instructions on the pipette bottom) lights red critical error, switch off ASSIST PLUS with On/ Standby key or Main switch On/Standby lights white ASSIST PLUS is switched on...

  • Page 10: Overview Of Integra Electronic Pipettes

    ASSIST PLUS – Operating instructions V02 Description of the device Overview of INTEGRA Electronic Pipettes 2.3.1 Pipette parts 24 Display 25 Back button, to navigate backward 26 Touch wheel, spin to scroll and move the cursor 27 OK button, to make a selection...

  • Page 11: Chapter 3 Installation

    Installation Operating environment ASSIST PLUS has been designed for use in a laboratory. It shall be operated in a dry and dust-free location with a temperature of 5–40°C and a maximal (non-condensing) relative humidity of 80 %, see “7.1 Environmental conditions” on page 43.

  • Page 12: Removing The Tip Ejector Lid

    (34) with your thumb nail and store it in a safe place (pipettes with serial number ≥7 000 000 only). Adapting the pipette holder The pipette holder can be adapted to accommodate INTEGRA electronic multichannel pipettes. To adapt the holder for an 8- or 16-channel VIAFLO pipette fold both silver levers (a, b) down as shown beside.

  • Page 13: Inserting Decks

    ASSIST PLUS – Operating instructions V02 Installation To release the pipette lift the black gripper by pushing down the left end. Inserting decks Hold the front and the back of the Tip box deck (6), find the correct orientation and place it over the corresponding positioning studs.

  • Page 14: Toolbox Settings

    Select Preferences and then Main Menu. Enable the ASSIST PLUS program by pressing OK (green ) and press  to save your settings. 3.9.2 Overview of Toolbox for ASSIST PLUS The Toolbox for ASSIST PLUS provides options to adapt the pipette to appropriate applications. Toolbox Description...

  • Page 15: Bluetooth Pairing

    Electronic Pipettes of 12.5, 300 and 1250 µl sizes can be used with standard, SHORT and LONG GripTips. In order to teach the correct heights for ASSIST PLUS movements, the used tip type must be specified. Go to the Toolbox of the pipette, select ASSIST PLUS and press OK.

  • Page 16: Position Adjustment

    Position adjustment The Position Adjustment option is used to set an offset for the absolute X/Y/Z-coordinates in mm to adjust the tip rack position. Select ASSIST PLUS on the Toolbox and press OK. Select Position Adjustment and press OK. Use the Touch wheel to select Tip Rack and press Set the offset(s) in mm of the selected option and Save ...

  • Page 17: Chapter 4 Operation

    After homing the LED of the Start/Pause key will turn off. To switch ASSIST PLUS to standby press the On/Standby key (19) for two seconds until its LED dims. The ASSIST PLUS automatically switches in standby program after 2 hours of inactivity.

  • Page 18: Via Communication Cable

    You are prompted to place the pipette on the ASSIST PLUS device, see “3.6 Attaching and removing an electronic pipette” on page 12. Press the blinking Start/Pause key (17) on the ASSIST PLUS device. It switches to solid white, the ASSIST PLUS homes and the program will be performed automatically.

  • Page 19: Troubleshooting

    ASSIST PLUS – Operating instructions V02 Operation Troubleshooting Problem Probable cause Remedy The connection • The two instruments have not • From the main menu of the between pipette and been paired or the pairing pipette go to Toolbox -> ASSIST ASSIST PLUS can- was lost.

  • Page 20: Chapter 5 Programming

    Please refer to www.integra-biosciences.com/download-vialab for more information. The table below shows the ASSIST PLUS program categories: VIALAB programs, predefined programs (Serial Dilution, Repeat Dispense, Variable Dispense, Multi Aspirate, Plate Copy, Reformat) and custom programs.

  • Page 21: Setting Up A Program Directly On The Pipette

    ASSIST PLUS – Operating instructions V02 Programming Setting up a program directly on the pipette Use the Touch wheel to scroll to your desired pipetting category and press OK. If no Bluetooth connection is available, you can also use the offline mode to create a new program.

  • Page 22: Adjust Heights And Positions

    Open a predefined or a custom program. When the setting dial is displayed as described above, use the ASSIST PLUS Touch panel (12): • Press the  Left and  Right keys to move the GripTips into the desired X-position.

  • Page 23: Program Offset

    Select Height Adjust, a step and scroll to a Height [1/n]. Press  Move to pos. and press the Start/Pause key on ASSIST PLUS to move the pipette to the programmed position. Use the Arrow keys (, ) to adjust the Height.

  • Page 24: Pipetting Alternating Wells

    Programming Pipetting alternating wells The pipetting arm of ASSIST PLUS moves in Y-direction (to the rear or the front) to accommodate even and odd rows/columns of plates using a pipette that does not correspond directly to the row/column number. For example, pipetting into all 16 wells per column of a 384 well plate with an 8-channel pipette.

  • Page 25: Detailed Description Of Predefined Programs

    ASSIST PLUS – Operating instructions V02 Programming Detailed description of predefined programs Use the Touch wheel to select an option, define the required parameters and press OK. Press OK to toggle between on (green ) and off (red ). All heights are defined relative to the deck, see “5.4 Adjust heights and positions”...
  • Page 26 ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Serial Dilution Source Type Sets the source container where the initial sample is (Reser- aspirated from (Reservoir or Plate). Only INTEGRA voir) reservoirs can be used as Reservoir type.
  • Page 27 ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Serial Dilution Target Plate Sets the destination plate position (B or C). Clear Height Sets the travel height at which the GripTips move from well to well on the target plate. Note: only visible if not yet defined under source.
  • Page 28 ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Serial Dilution Advan- Tip Travel Defines the distance which the tips move during an aspira- tion, dispense and mix step to artificially track the liquid level, see “7.8 Tip travel distances” on page 47.

  • Page 29: Repeat/Variable Dispense Programs

    Source Type Sets the source container where the initial sample is (Reser- aspirated from (Reservoir or Plate). Only INTEGRA reser- voir) voirs can be used in the Reservoir mode. Tracking If Reservoir is selected and Tracking is active, the pipette automatically adjusts the pipetting height according to the defined tip immersion depth.
  • Page 30 Options Steps Description of Repeat/Variable Dispense Source Start Height The height where aspiration starts. The ASSIST PLUS will (Gene- automatically refill the GripTips if the total dispense volume ral) required for the target exceeds the maximum volume of the pipette. The first aspiration will be at the start height and for consecutive aspiration steps the pipette will go lower until it reaches the end height.
  • Page 31 ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Repeat/Variable Dispense Target TipTouch It is highly recommended to activate a tip touch after a dispensing step to remove drops that may cling to the pipette tips. • Sets where the tip touch should be performed.

  • Page 32: Multi Aspirate Program

    ASSIST PLUS – Operating instructions V02 Programming 5.7.3 Multi aspirate program Application: This program can be used for pooling applications or removal of supernatants. Options Steps Description of Multi aspirate Plate Wells Sets the type of well plate (12, 24, 48, 96 or 384) to be used as (source)/target.
  • Page 33 ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Multi aspirate Target Type Sets the target container where the sample is dispensed (Reservoir or Plate). Plate If plate, set the destination plate position (B or C). Column/Row Select the column/row of the plate where the sample is dispensed.

  • Page 34: Plate Copy Program

    ASSIST PLUS – Operating instructions V02 Programming 5.7.4 Plate copy program Application: This program can be used to copy all columns/rows from plate A to plate B with optional tip change after each transfer. The well number and the orientation of the plate must be the same.

  • Page 35: Reformat Program

    ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Plate Copy Advan- Tracking Chooses if the pipette automatically follows the liquid level, see “7.8 Tip travel distances” on page 47. • Source Asp: moves the set distance down during aspira- tion from the source location.
  • Page 36 ASSIST PLUS – Operating instructions V02 Programming Options Steps Description of Reformat Aspirate Aspirate Sets the aspiration volume. Asp. Speed Sets speed uniquely for aspiration (1 = low, 10 = fast). Height Sets the height for the aspiration step. Mixing Mixes the sample in the source plate before the first aspira- tion.

  • Page 37: Custom Programs

    Custom programs can contain up to 98 steps. From the ASSIST PLUS Menu select “Custom Programs”, press OK and New . Define and save a name for your program. The first line is highlighted. Press OK.
  • Page 38 Moves the pipette in Z direction to the selected coordinate (the higher the value, the longer is the distance between pipette tip and deck). Use the Arrow keys on ASSIST PLUS to teach the position or dial the height manually on the pipette’s screen. Press OK on the pipette to accept the settings.
  • Page 39 ASSIST PLUS – Operating instructions V02 Programming Example of custom program Application: The task is to aspirate liquid from a 100 ml reservoir with a 300 µl pipette and fill the first 6 columns of a 96 well plate with 50 µl. The custom program would be set...
  • Page 40 ASSIST PLUS – Operating instructions V02 Programming Programming errors Problem Probable cause Remedy Z-Speed (ASSIST) • Tracking distance of Aspirate, • Increase tracking distance or too low! Increase Dispense or Mix step is too pipetting speed. distance or pipetting short for the current pipetting speed.

  • Page 41: Chapter 6 Maintenance

    Decontamination From regular use ASSIST PLUS should not come into direct contact with liquids. If aerosols or biohazardous fluids splash on the surfaces, they must be decontaminated in accordance to good laboratory practice.

  • Page 42: Servicing

    Equipment disposal The ASSIST PLUS must not be disposed of with unsorted municipal waste. Dispose the ASSIST PLUS in accordance with the laws and regulations in your area governing disposal of devices. INTEGRA Biosciences AG...

  • Page 43: Chapter 7 Technical Data

    Tower, waste bin, sensor: painted stainless steel Decks: anodized aluminum Touch panel: glass Sensor reflector: Polymethylmethacrylate (PMMA), aluminum Intellectual property The ASSIST PLUS is covered under the following patents: Patent Number Country Title Apply to 9,321,048 Sample Distribution System And Pro- ASSIST,...

  • Page 44: Compatible Pipettes

    ASSIST PLUS – Operating instructions V02 Technical Data Compatible pipettes The following INTEGRA pipettes are compatible with ASSIST PLUS: Pipette Firmware Serial No. VIAFLO 4.xx 7xxxxxx VOYAGER 4.xx 7xxxxxx Maximum labware height on deck The maximum labware height defines the distance between pipette tip end and deck (in...

  • Page 45: Plate Compatibility

    ASSIST PLUS – Operating instructions V02 Technical Data Plate compatibility The tables below provide an overview outlining the compatibilities of pipettes with different well plates and plate orientations. VIAFLO pipettes 96 well plate (8 x 12) 384 well plate (16 x 24)

  • Page 46: Plate Access Pattern

    ASSIST PLUS – Operating instructions V02 Technical Data Plate access pattern Plates can be reformatted in two different patterns using the predefined Reformat program. 96 wells 384 wells Reformat four 96 well source plates with an 8 channel VOYAGER pipette into 4...

  • Page 47: Tip Travel Distances

    ASSIST PLUS – Operating instructions V02 Technical Data Tip travel distances The Tip Travel defines the distance which the tips move during an aspiration, dispense and mix step to artificially track the liquid level. The Tip Travel starts from a user defined height as shown below.

  • Page 48: Chapter 8 Accessories

    ASSIST PLUS – Operating instructions V02 Accessories Accessories Accessories General Part No. Pipette Li-ion battery 4205 Bluetooth module for INTEGRA electronic pipettes 4221 Communication/charging cable pipette to ASSIST PLUS 4548 Pipette charging cable 4549 Charging/communication stand for 1 pipette, incl. mains adapter 4211 Tip waste bin (incl.
  • Page 49 ASSIST PLUS – Operating instructions V02 Accessories VIAFLO Electronic Pipettes Part No. 8-channel pipette, 0.5 – 12.5 μl 4621 8-channel pipette, 2 – 50 μl 4626 8-channel pipette, 5 – 125 μl 4622 8-channel pipette, 10 – 300 μl 4623 8-channel pipette, 50 –...

  • Page 50: Consumables

    ASSIST PLUS – Operating instructions V02 Accessories Consumables 10 ml Disposable Reagent Reservoirs for multichannel pipettes Part No. 10 ml Reservoir Base, 10 pack 4306 Polystyrene Trial pack (1 base and 3 reservoirs), sterile 4330 30 reservoirs individually sealed, sterile, 1 base per case...
  • Page 51 ASSIST PLUS – Operating instructions V02 Accessories 25 ml Disposable Reagent Reservoirs for multichannel pipettes Part No. 25 ml Reservoir Base, 10 pack 4304 Polystyrene Trial pack (1 base and 3 reservoirs), sterile 4310 30 reservoirs individually sealed, sterile, 1 base per case...
  • Page 52 ASSIST PLUS – Operating instructions V02 Accessories 100 ml Disposable Reagent Reservoirs for multichannel pipettes Part No. 100 ml Reservoir Base, 10 pack 4305 Polystyrene Trial pack (1 base and 3 reservoirs), sterile 4320 30 reservoirs individually sealed, sterile, 1 base per case...
  • Page 53 ASSIST PLUS – Operating instructions V02 Accessories Automation friendly Clear Advantage™ reagent reservoirs Part No. 300 ml 8 reservoir bases, automation friendly (microplate 6305 format), non-sterile 25 lids, sterile, polypropylene 6306 Polystyrene Trial pack (1 base, 1 reservoir and 1 lid), polystyrene,...
  • Page 54 ASSIST PLUS – Operating instructions V02 Accessories GripTips for automation of INTEGRA Pipettes Part No. Purple: 5 XYZ racks of 384 tips, non-sterile 6453 12.5 µl 5 XYZ racks of 384 tips, sterile 6454 5 XYZ racks of 384 tips, sterile, filter...
  • Page 55 ASSIST PLUS – Operating instructions V02 Accessories GripTips for automation of INTEGRA Pipettes Part No. Green: 5 racks of 96 tips, non-sterile 6533 300 μl 5 racks of 96 tips, sterile 6534 low retention 5 racks of 96 tips, sterile, filter...

  • Page 56: Imprint

    © 2019 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.

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