Integra VIAFLO ASSIST 4500 Operating Instructions Manual
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Summary of Contents for Integra VIAFLO ASSIST 4500
- Page 1 VIAFLO ASSIST Operating instructions 127950_V00...
- Page 2 Déclaration de conformité | Declaración de conformidad | Dichiarazione di conformità INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the product | erklärt in alleiniger Verantwortung, dass das Produkt | déclare sous sa responsabilité exclusive, que le produit | declara bajo su propia responsabilidad que el producto | dichiara sotto la propria responsabilità...
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Page 3: Table Of Contents
4.2.1 Overview pipetting modes...........14 4.2.2 General parameters of all pipetting modes ......15 4.2.3 Serial dilution mode ............16 4.2.4 Repeat dispense mode ............18 4.2.5 Variable dispense mode .............20 4.2.6 Custom program mode ............21 4.3 Modify existing programs ...............22 4.4 Troubleshooting................23 www.integra-biosciences.com... - Page 4 5.2 Decontamination ................24 5.3 Servicing..................24 Chapter 6 Technical Data 6.1 Environmental conditions ...............25 6.2 Specification of the device..............25 Chapter 7 Accessories 7.1 Accessories for VIAFLO Electronic Pipettes ........26 7.2 Accessories for VIAFLO ASSIST ...........26 7.3 Consumables .................27 INTEGRA Biosciences AG...
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Page 5: Chapter 1 Introduction
VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5– 1250 µl using GripTip pipette tips. Please refer to the VIAFLO II Electronic Pipettes operating instructions for more detailed informations on www.integra-biosciences.com. www.integra-biosciences.com... -
Page 6: Safety Notes
Do not open or modify the VIAFLO ASSIST in any way. Repairs may only be performed by INTEGRA Biosciences AG or by an authorised after-sales service member. Parts may be replaced with original INTEGRA Biosciences parts only. -
Page 7: Description Of The Device
12 Start/Pause key, to start/stop operations. Green LED blinks: press to start operation/homing Green LED lights: operation performed 13 Bluetooth control LED (lights blue if connection is active) and Error LED (flashes red in case of error) 14 On/Off key (lights green if On) www.integra-biosciences.com... -
Page 8: Overview Of Viaflo Ii Electronic Pipette
23 Finger hook, facilitates easy operation 24 Volume indicator label, color matches GripTip box insert. 2.3.2 Display The Display shows all pipetting options. Battery life indicator OK - Serial Dilution, select program Current mode Instruction Active step Volume Options INTEGRA Biosciences AG... -
Page 9: Chapter 3 Installation
The pipette holder can be adapted to the type of the VIAFLO II Electronic Pipette. To adapt the holder for an 8-channel pipette, push the silver lever down as shown beside. The width of the pipette holder is now reduced. Lift the lever upwards, if a 12-channel pipette should be inserted. www.integra-biosciences.com... -
Page 10: Attaching And Removing A Viaflo Ii Electronic Pipette
The ASSIST mode is hidden from the Main Menu of the VIAFLO pipettes by default. Use the Touch wheel to select Toolbox from the Main Menu and press OK. Select Preferences and then Main Menu. Enable the ASSIST mode by pressing OK (green tick) and press to save your settings. INTEGRA Biosciences AG... -
Page 11: Setting Tip Type
It is possible to create programs, however without teaching of active heights. Press the Start/Pause key when it is blinking to home the VIAFLO ASSIST device. ARNING Remove hands from VIAFLO ASSIST during homing. After homing the LED of the Start/Pause key will turn off. www.integra-biosciences.com... -
Page 12: Adjusting The Plate Alignment
When it turns red, the pipette needs to be charged. AUTION Use only the approved INTEGRA battery, power supply or charging stand. Use of an incompatible power transformer can damage the pipette. The battery can be charged using either the Universal Voltage Power Supply (UPS) or a charging stand, see (“7.1 Accessories for VIAFLO Electronic Pipettes”... -
Page 13: Chapter 4 Operation
Keep hands out of area of moving VIAFLO ASSIST parts during the run. A program can be interrupted by pressing the Start/Pause key (12). Either press the Start/Pause key again to continue the program or press Abort on the pipette to abort the program. www.integra-biosciences.com... -
Page 14: Setting Up A Program
“4.3 Modify existing programs” on page 22. Define all parameters of your program and press Save . To run the program, select the stored program and press OK on the VIAFLO Electronic Pipette, see “4.1 Running a program” on page 13. INTEGRA Biosciences AG... -
Page 15: General Parameters Of All Pipetting Modes
GripTips to the desired height above the plate. • Alternatively, use the Touch wheel to enter the height directly, if known (distance between Plate sledge (2) and Tip end). Press OK on the pipette to save current settings. www.integra-biosciences.com... -
Page 16: Serial Dilution Mode
Set the destination for the first transfer of the serial dilution (column 1-24 or First Row or row A-P). Rows or Set the number of rows or columns to dilute (1-n, including the first row/ Columns column). INTEGRA Biosciences AG... - Page 17 GripTips at the correct blow out height and press OK. • Alternatively, use the Touch wheel to enter the height directly, if known, and press OK. Press to save your settings. This will return you to the list of Serial Dilution programs. www.integra-biosciences.com...
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Page 18: Repeat Dispense Mode
Set the aspiration speed (1 = low, 10 = fast). Mixing If required, set a mixing step before aspiration. • Press OK to toggle between ON (green tick) and OFF (red cross, default). • If ON, define Mix volume, Mix Speed and number of Mix Cycles. INTEGRA Biosciences AG... - Page 19 • If ON, define the volume of Last Dispense and the blowout height. Set the Blowout height below the liquid in the wells for best results. Press to save your settings. This will return you to the list of Repeat Dispense programs. www.integra-biosciences.com...
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Page 20: Variable Dispense Mode
Option Description Dispense Set the different dispense volumes for every repeat dispense step. 1 to n Press to save your settings. This will return you to the list of Variable Dispense programs. INTEGRA Biosciences AG... -
Page 21: Custom Program Mode
Moves the pipette in Z direction to the selected coordinates. Use the Arrow keys on VIAFLO ASSIST to teach the position or dial the height manually on the pipette’s screen. Press OK on the pipette to accept the settings. www.integra-biosciences.com... -
Page 22: Modify Existing Programs
Modify existing programs At any program display, use the Touch wheel to highlight an existing program. Press Options, use the Touch wheel to select an option (View/Edit, Delete, Copy, Rename) to modify the program and press OK. INTEGRA Biosciences AG... -
Page 23: Troubleshooting
X-posi- “3.6.4 Adjusting the plate tion. alignment” on page 12 The tip height is not • Wrong tip type set. • Enter the tip type used, see correct using 12.5 μl “3.6.2 Setting tip type” on GripTips. page 11 www.integra-biosciences.com... -
Page 24: Chapter 5 Maintenance
If working with infectious materials, e. g. human pathogens, VIAFLO ASSIST needs to be decontaminated before sending it to service and the declaration on the absence of health hazards must be signed. This is necessary to protect service personnel. INTEGRA Biosciences AG... -
Page 25: Technical Data
40 °C. Specification of the device Power supply Input: 100–240VAC, 50/60Hz Output: 24 VDC, 3.0 A Dimensions (H x D x W) 400 mm x 360 mm x 340 mm (with pipette attached: height 510 mm) Weight 10 kg www.integra-biosciences.com... -
Page 26: Chapter 7 Accessories
8-channel pipette, 10.0–300.0 μl 4623 8-channel pipette, 50.0–1250 μl 4624 12-channel pipette, 0.50–12.50 μl 4631 12-channel pipette, 5.0–125.0 μl 4632 12-channel pipette, 10.0–300.0 μl 4633 12-channel pipette, 50.0–1250 μl 4634 16-channel pipette, 0.50–12.50 μl 4641 16-channel pipette, 5.0–125.0 μl 4642 INTEGRA Biosciences AG... -
Page 27: Consumables
4312 per case, sterile Reservoir Base, 10 pack 4304 100 ml Disposable reagent reservoirs, individually sealed, 4321 30 reservoirs per case, sterile Disposable reagent reservoirs, four sleeves of 50 reservoirs 4322 per case, sterile Reservoir Base, 10 pack 4305 www.integra-biosciences.com... - Page 28 5 inserts of 96 tips, non-sterile, GREEN CHOICE 4442 5 boxes of 96 Tips, non-sterile 4443 5 boxes of 96 Tips, sterile 4444 5 boxes of 96 Tips, sterile, filter 4445 5 inserts of 96 tips, pre-sterilized, GREEN CHOICE 4446 INTEGRA Biosciences AG...
- Page 29 © 2013 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
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