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Table of Contents
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Summary of Contents for Integra DOSE IT
- Page 1 DOSE IT Operating instructions 171250_V08...
- Page 2 Declaration of conformity INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the device Description Model DOSE IT P910 171 000 complies with: EU Directives (DoW: Date of Withdrawal) Before DoW After DoW Low Voltage Equipment 2006/95/EC 20.04.2016...
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Page 3: Table Of Contents
DOSE IT – Operating instructions V08 Table of contents Chapter 1 Introduction 1.1 Intended use ..................5 1.2 Symbols used.................5 1.3 Safety notes ...................6 Chapter 2 Description of the device 2.1 Scope of delivery................7 2.2 Overview of the DOSE IT...............7 Chapter 3 Installation 3.1 Power supply connection ...............9... - Page 4 DOSE IT – Operating instructions V08 Chapter 7 Accessories and consumables 7.1 Consumables ................27 7.2 Spare part ..................28 7.3 Accessories..................29 Imprint ....................30 INTEGRA Biosciences AG...
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Page 5: Chapter 1 Introduction
The programmable peristaltic pump DOSE IT is designed for pumping and dispensing liquids in a volume range of 0.1 ml to 9999 ml using silicone tubings. If the DOSE IT is used in a manner not specified by INTEGRA, the protection provided by the DOSE IT may be impaired. -
Page 6: Safety Notes
DOSE IT – Operating instructions V08 Introduction Safety notes The DOSE IT conforms to the state of technology and the recognized safety rules, and is operationally safe. Operate the DOSE IT when in perfect conditions only and observing this user manual. -
Page 7: Chapter 2 Description Of The Device
DOSE IT – Operating instructions V08 Description of the device Description of the device Scope of delivery • DOSE IT P910 peristaltic pump • Silicone tubing, autoclavable, 4 mm inner diameter (ID) • 2x Aspiration/dispensing tubes 10 cm, stainless steel, 4 mm ID, ... - Page 8 Connections and mains switch 10 RS-232 interface for connection with serial printer 11 RS-232 interface for service connection 12 AUX connection for controlling a second DOSE IT 13 Mains switch 14 Connection for foot switch, benchtop switch, MEDIAJET or external relay contact (potential free, normally open, 3.5 mm jack plug) for remote control...
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Page 9: Chapter 3 Installation
“2.1 Scope of delivery” on page 7. Should something be missing or should you find a faulty component, please contact your local INTEGRA Biosciences representative. Power supply connection Connect the AC adapter cable to the DOSE IT mains connection socket and plug it to the electricity mains. ARNING ... -
Page 10: Chapter 4 Operation
4.1.1 Tubing selection The pumphead of the DOSE IT is compatible with differently sized tubing allowing the user to dispense a wide range of dose volumes. The main criteria for the choice of tubing are the speed and precision requirements of a specific application. The larger the inner diameter (ID) of the tubing, the faster but less precise is the dispensing. - Page 11 Operation 4.1.2 Loading of the tubing in the pumphead ARNING Always switch off the DOSE IT when loading the tubing and when manipulating the open pumphead. 1 Open the pumphead 2 Adjust the tubing clamps The jagged, V-shaped tubing clamps on both sides of the pumphead need to be adjusted and guarantee that the tubing does not slip when the rotor turns.
- Page 12 The aspiration/dispensing tubes delivered with the Silicone tubing DOSE IT facilitate the dosing process and are fitted on the end of the tubing. The aspiration tube is dented on one end in order to guarantee a free flowing of the liquid Aspiration/ entering the tubing.
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Page 13: Parameter Settings
DOSE IT – Operating instructions V08 Operation Parameter settings 4.2.1 System parameters System-Parameter Description Language Set the desired language. Time/Date Set time and date. Display contrast Change the contrast of the display. Access code Provides user access restrictions to parameters. - Page 14 If the speed of the follow- Pause ing step is identical to the end flow, and the pause is set to Start flow manual, the DOSE IT continues without pause. End flow INTEGRA Biosciences AG...
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Page 15: Adjusting The Dispensing Program
4.3.1 Choosing the language Because it is easiest to operate the DOSE IT by following the instructions on the screen, please select the language you are most familiar with. 1 Switch on the DOSE IT by pressing the mains switch at the back.... - Page 16 4.3.2 Access code The DOSE IT system and parameter settings can be protected by a code, if activated (Access code required ON). Before any parameter changes can be performed, an access code must be entered. A standard user can only run defined programs.
- Page 17 DOSE IT – Operating instructions V08 Operation 4.3.4 Defining a customized program The following example of a customized program can be used to fill 10 density gradient tubes. The careful and continuous dispensing without pauses prevents stirring up of layers.
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Page 18: Calibrating The Pump
DOSE IT – Operating instructions V08 Operation Calibrating the pump The calibration procedure tunes the pump to deliver a precise output. The output of the pump is determined by the tubing's inner diameter; therefore a calibration is necessary for tubing with different inner diameter. Also calibrate the pump after changing the fluid to be pumped. -
Page 19: Performing The Dispensing Process
DOSE IT – Operating instructions V08 Operation Performing the dispensing process 1 In the A window select a program and press PPLICATION to access the program window. Press P to fill TART RIME the tubing with liquid until no more bubbles are in the tube and calibrate if necessary. -
Page 20: Using The Double Pumphead Configuration (Optional)
Operation Using the double pumphead configuration (optional) The DOSE IT can be used with a double pumphead and the corresponding 8 mm ID tubing set (171 088) when it is necessary to quickly dispense large volumes. The double pumphead configuration also reduces flow pulsation. -
Page 21: Process Documentation (Optional)
Operation Process documentation (optional) To document the dispensing and pumping performed with a DOSE IT, relevant data can be recorded either on paper or in an electronic text file. The following data – exemplified by a dispensing program – are recorded: •... -
Page 22: Using The Foot And Benchtop Switch
INTEGRA dealer can assist you to connect this printer. To generate an electronic text file, connect a PC to the RS - 232 (2) port of the DOSE IT via a crossed female-female RS - 232 cable and record the process data using the HyperTerminal software installed on your PC. -
Page 23: Chapter 5 Maintenance
Always switch off the DOSE IT and disconnect it from the mains when carrying out maintenance work. If the DOSE IT gets soiled, it can be cleaned with a cloth moistened with soapy water or with a 70 % solution of Ethanol. -
Page 24: Disposal
Maintenance If the system is leaking, you have to check whether the tubing or the pumphead is worn: Repeat the leak test with a new tubing (only use original INTEGRA tubings, see “7.1 Consumables” on page 27). • If system is tight, the tubing was worn and the problem is solved. -
Page 25: Chapter 6 Technical Data
DOSE IT – Operating instructions V08 Technical data Technical data Environmental conditions Operation Temperature range 5–35 °C Humidity range Max. rel. humidity 80 % for temperatures up to 31 °C, decreasing linearly to 50 % rel. humidity at 40 °C. -
Page 26: Chemical Compatibility
The table below rates the compatibility of silicone to a few of the chemicals commonly used in laboratories. INTEGRA Biosciences AG assumes no liability for the information contained in the table. Chemical... -
Page 27: Chapter 7 Accessories And Consumables
DOSE IT – Operating instructions V08 Accessories and consumables Accessories and consumables There are a number of accessories and options that adapt the DOSE IT to particular application requirements and working environments. Consumables 7.1.1 Silicone tubing Part No. (mm) length 2.5 m, autoclavable, ... -
Page 28: Spare Part
DOSE IT – Operating instructions V08 Accessories and consumables 7.1.2 Aspiration/dispensing tubes ID (mm) Part No. Aspiration/dispensing tube, length 10 cm, 171 051 stainless steel, one end dented. 171 052 171 053 171 054 171 056 171 058 Aspiration/dispensing tube, length 35 cm,... -
Page 29: Accessories
DOSE IT – Operating instructions V08 Accessories and consumables Accessories Part No. Foot switch. 143 200 Benchtop switch. 171 081 Retort rod with filling arm. 171 091 Extension pumphead for double pumphead assembly. 171 090 See also “4.7 Using the double pumphead configuration (optional)”... -
Page 30: Imprint
© 2018 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
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