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Document No. 032882-05
SECTION 4 - EXAMPLE APPLICATIONS
Separation and elution of common anions on the IonPac Fast Anion Analytical Column has been optimized utilizing a specific
carbonate/bicarbonate eluent. Both monovalent and divalent anions are eluted in less than three minutes based on the concentration
and proportions of sodium carbonate and sodium bicarbonate. The Suppressor reaction product is carbonic acid (H
results in a low background conductivity.
4.1
Eluent Preparation
The 2.00 mM Na
CO
/0' 15 mM NaHC0
2
3
chromatographer to easily and precisely prepare large volumes of eluent while at the same time using a minimum amount of storage
space.
Since the IonPac Fast Anion Column is susceptible to irreversible damage from eluents with pH outside the range of 2 and 11, the
100X eluent concentrate is also recommended for column cleanup (see, "Column Care").
4.1.1 Preparation Of 100x Eluent Concentrates - 0.5 M Na
A 100X concentrate can be made from either reagent grade chemicals or from DIONEX eluent concentrates.
Do not use hydroxide to adjust the pH of the eluent in order to adjust the ion exchange selectivity of the system. Using
hydroxide in the eluent may cause irreversible damage to the IonPac Fast Anion Analytical Column.
USING REAGENT GRADE SALTS
A. Weigh 10.6 g of anhydrous Na
B. Add 250 mL of degassed, deionized water having a specific resistance of 18.2 megohm-cm to the flask and thoroughly
dissolve the Na
CO
2
C. Dilute to a final volume with degassed, deionized water having a specific resistance of 18.2 megohm-cm.
USING DIONEX ELUENT CONCENTRATES
It is possible to dilute the DIONEX 0.5 M Na
037163) directly to make the eluents, but the dilution factor is so large that the precise duplication of the diluted eluent
may be difficult to achieve from one dilution to the next. For the sake of precision and ease of preparation, DIONEX
recommends that the 100X eluent concentrates be prepared from the 0.5 M concentrates for use in making the eluents
A. Add 200 mL of 0.5 M Na
mL volumetric flask.
B. Add 250 mL of degassed, deionized water having a specific resistance of 18.2 megohm-cm to the flask and thoroughly
dissolve the Na
CO
2
C. Dilute the final volume of 500 mL degassed, deionized water having a specific resistance of 18.2 megohm-cm.
eluent is prepared daily by the dilution of a 100X eluent concentrate. This allows the
3
CO
and 0.63 g of NaHCO
2
3
and the NaHCO
.
3
3
CO
2
CO
concentrate (P/N 037162) and 15 mL of 0.5 M NaHCO
2
3
and the NaHCO
3
3.
IonPac Fast Anion
CO
/0.0075 M NaHCO
2
3
NOTE
into a 500 ml- volumetric flask.
3
concentrate (P/N 037162) and the 0.5 M NaHCO
3
Page 6 of 12
CO
2
3
concentrate (P/N
3
concentrate (P/N 037163) in a 500
3
) which
3
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