Thermo Scientific Dionex IonPac Product Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Measuring Instruments
  5. Dionex IonPac
  6. Product manual
Thermo Scientific Dionex IonPac Product Manual

Thermo Scientific Dionex IonPac Product Manual

Fast anion analytical column 4 x 250 mm
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
PRODUCT MANUAL
IONPAC
FAST ANION ANALYTICAL COLUMN
®
(4 x 250 mm, P/N SP5516)
QUICKSTART STEPS AND LINKS
Click blue text below to get started.
1.
See
Section 3, "Operation".
requirements.
2.
See
Section 4.1, "Eluent
for eluents.
3.
See
"Quality Assurance
Report". Run the Production Test Chromatogram as a system
check.
4.
See
Section 4.3, "Standard Operating Conditions"
5.
See
"Column Care"
for column cleanup and long-term storage recommendations.
©DIONEX Corporation, 1997, 1998, 2002
Note operation precautions and chemical purity
Preparation". Make the required stock and working solutions
for an example application.
Document No. 032882
Revision 05
7 November 2002

Advertisement

Table of Contents
loading

Related Manuals for Thermo Scientific Dionex IonPac

Summary of Contents for Thermo Scientific Dionex IonPac

  • Page 1 PRODUCT MANUAL IONPAC FAST ANION ANALYTICAL COLUMN ® (4 x 250 mm, P/N SP5516) QUICKSTART STEPS AND LINKS Click blue text below to get started. Section 3, “Operation”. Note operation precautions and chemical purity requirements. Section 4.1, “Eluent Preparation". Make the required stock and working solutions for eluents.

  • Page 2: Table Of Contents

    Document No. 032882-05 IonPac Fast Anion Page 2 of 12 TABLE OF CONTENTS SECTION 1 - INTRODUCTION ....................... 3 SECTION 2 - INSTALLATION ......................4 System Requirements ............................... 4 SECTION 3 - OPERATION ....................... 5 Operation Precautions .............................. 5 Chemicals Required ..............................5 SECTION 4 - EXAMPLE APPLICATIONS ..................

  • Page 3: Section 1 - Introduction

    Document No. 032882-05 IonPac Fast Anion Page 3 of 12 SECTION 1 - INTRODUCTION The IonPac Fast Anion Analytical Column (P/N 039590) is designed specifically for the rapid analysis of anions in defined matrices. It provides the most rapid analysis available. Seven common anions can be determined in less than 3 minutes. It is ideal for achieving maximum throughput of relatively simple, well-characterized samples.

  • Page 4: Section 2 - Installation

    Document No. 032882-05 IonPac Fast Anion Page 4 of 12 SECTION 2 - INSTALLATION System Requirements The IonPac Fast Anion Analytical Column must be run on a Series 2000i or 4000i Ion Chromatograph (IC) equipped with an Anion MicroMembrane Suppressor, AMMS III, P/N 056750) and a Conductivity Detector. NOTE To save regenerant preparation time, consumption, and waste, it is recommended that the AutoRegen Accessory be purchased.

  • Page 5: Section 3 - Operation

    Document No. 032882-05 IonPac Fast Anion Page 5 of 12 SECTION 3 - OPERATION Operation Precautions A. The eluent pH should be between 2 and 11. The sample pH should be between 2 and 13. Exceeding the limits of these pH ranges will cause Irreversible damage to the IonPac Fast Anion Analytical Column.

  • Page 6: Section 4 - Example Applications

    Document No. 032882-05 IonPac Fast Anion Page 6 of 12 SECTION 4 - EXAMPLE APPLICATIONS Separation and elution of common anions on the IonPac Fast Anion Analytical Column has been optimized utilizing a specific carbonate/bicarbonate eluent. Both monovalent and divalent anions are eluted in less than three minutes based on the concentration and proportions of sodium carbonate and sodium bicarbonate.

  • Page 7: Dilution Of The Eluents Concentrate

    Document No. 032882-05 IonPac Fast Anion Page 7 of 12 4.1.2 Dilution of the Eluents Concentrate Dilute 10.0 mL of 100X eluent concentrate to one liter in a volumetric flask, using degassed, deionized water having a specific resistance of 18.2 megohm-cm. The eluent is now ready for use. Regenerant Preparation The regenerant is 20 mN sulfuric acid.

  • Page 8: Standard Operating Conditions

    Document No. 032882-05 IonPac Fast Anion Page 8 of 12 Standard Operating Conditions Sample Loop Volume: 20 µL Analytical Column: IonPac Fast Anion Analytical Column Eluent: 2.00 mM Na 0. 15 mM NaHCO Eluent Flow Rate: 2.0 mL/min MMS Suppressor: Anion MicroMembrane Suppressor, AMMS III MMS Regenerant: 20 mNH...

  • Page 9: Section 5 - Troubleshooting Guide

    Document No. 032882-05 IonPac Fast Anion Page 9 of 12 SECTION 5 - TROUBLESHOOTING GUIDE The purpose of the Troubleshooting Guide is to help you solve operating problems that may arise while using the IonPac Fast Anion Analytical Column. For more information on problems that originate with the Ion Chromatograph (IC) or the Anion MicroMembrane Suppressor (AMMS III), refer to the Troubleshooting Guide in the appropriate operator’s manual.

  • Page 10: High Background, Noise

    Document No. 032882-05 IonPac Fast Anion Page 10 of 12 NOTE If any of the column packing becomes lodged between the end of the column and the bed support washer assembly, no amount of tightening will seal the column. Make sure that the washer and the end of the column are clean before screwing the end fitting back onto the column.

  • Page 11: Short Retention Times

    Document No. 032882-05 IonPac Fast Anion Page 11 of 12 B. Extra-column effects can result in sample band dispersion, making the peaks elution less efficient. Make sure you are using 0.01 2-inch ID tubing, in all cases, between the injection valve and the detector cell inlet, and that the tubing lengths are minimized.

  • Page 12: Baseline Upsets

    Document No. 032882-05 IonPac Fast Anion Page 12 of 12 5.4.2 Baseline Upsets When an injection valve or a column switching valve is actuated, the possibility of creating a baseline disturbance exists. This baseline upset can show up as a peak of varying size and shape. It will happen when the particular valve needs to be cleaned or retorqued (see system manual).
  • Page 13 ® IonPac Fast Anion Analytical (4 x 250 mm) Product No. SP5516 Serial No. : 0102 Pressure (PSI) : 780 Date : 2/24/00 2:16:48 PM 0102 Eluent: 2.0 mM Na /0.15 mM NaHCO Flow Rate: 2.0 mL/min 16.0 Detection: Suppressed Conductivity 14.0 ASRS-ULTRA ®...
  • Page 14 IonPac Fast Anion Column Care Document No. 032882C05 Page 1 of 2 COLUMN CARE RECOMMENDED OPERATION PRESSURES Operating a column above its recommended pressure limit can cause irreversible loss of column performance. The maximum recommended operating pressure for the IonPac Fast Anion Analytical Column is 4000 psi. COLUMN START-UP The column is shipped in eluent as the storage solution.
  • Page 15 IonPac Fast Anion Column Care Document No. 032882C05 Page 2 of 2 BASE-SOLUBLE CONTAMINANTS CAUTION Do not use the standard NaOH procedure described in other Dionex documentation for base cleanup of the IonPac Fast Anion Analytical Column. The standard procedure will destroy this column. A.

Table of Contents