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- Page 1 Trilogy Laboratory Fluorometer User’s Manual Version 1.2 September 15, 2010 P/N 998-7210 Turner Designs 845 W. Maude Ave. Sunnyvale, CA 94085 Phone: (408) 749-0994 FAX: (408) 749-0998 www.turnerdesigns.com...
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Page 3: Highlights Of The Trilogy Fluorometer
Highlights of the Trilogy Fluorometer Multifunctional, Sample Chamber accepts Compact and Modern snap-in Application Modules Design provides for Fluorescence, flexibility and precision Absorbance and Turbidity measurements Spreadsheet Interface Software provides real time display and logging of data to Excel Trilogy saves time by storing... -
Page 4: Table Of Contents
Appendix E – Recommended Measurement Practices Linear Range and Quenching Temperature Considerations Appendix F - Chlorophyll a Acidification and Non-Acidification calculations Appendix G - In vivo fluorescence measurements using Trilogy Trilogy Fluorometer User’s Manual P/N 998-7210 Page 4 of 38... -
Page 5: Getting Started
Infrared allows Turbidity to be measured at wavelengths that are not normally absorbed by organic matter thereby reducing susceptibility to interference by sample color. When properly calibrated, the Trilogy Fluorometer will read out the actual concentration of the solution. Optical Modules contain the necessary light source and filters for the relevant... -
Page 6: Setup
Trilogy power on step will complete successfully. 2. Turn on the On/Off switch located on the back of the Trilogy, see Figure 1. Verify the display becomes active, and shows the module selection screen, see Figure 3 – Module Selection. -
Page 7: Precautions
3. Select the module inserted, and touch “OK” on the confirming screen. 4. When the Home Screen is displayed (Fig. 4), you are now ready to use Trilogy in its Raw Mode (measurements are relative) or calibrate the Trilogy and the snapped-in Optical Application Module to make quantitative measurements. -
Page 8: Fluorometer Operation
Press down firmly to lock the Optical Application Module in place. You should hear or feel a click indicating the module has snapped into place. (See Figure 5.) 4. Close the lid and power ON the Trilogy. Use the touch screen to identify the type of Optical Application Module installed. -
Page 9: Measuring Samples
Section V for the Calibration Procedure. The Trilogy accommodates 10 x 10 mm methacrylate and polystyrene cuvettes (minimum 2 mL volume). Use 12 x 35 mm or 12 x 75 mm glass test tubes for measuring solvent applications, such as extracted chlorophyll, and use methacrylate cuvettes for UV water applications. - Page 10 PC when properly connected, (see Section VII). Please note the Trilogy does not store more than 20 measurements at one time. If more than 20 readings are taken, the oldest reading will be overwritten. Measurements are not stored between power cycles.
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Page 11: Absorbance Operation
3. Press down to lock the Absorbance Module in place. You should hear or feel a click indicating the module has snapped into place. 4. Close the lid and power ON the Trilogy. Select "Absorbance" from the list of options on the touch screen. -
Page 12: Measuring Samples
Touch "Mode" to select the unit of measure for absorbance. The available options include Absorbance units (Ab) and Transmittance (%T). The following formulas describe the method of the Trilogy Absorbance Module for measuring % transmittance and absorbance: %T = [(s-z) / (b-z)] * 100... - Page 13 PC if properly connected, (see Section VII). Please note the Trilogy does not store more than 20 measurements at one time. If more than 20 readings are taken, the oldest reading will be overwritten. Measurements are not stored between power cycles.
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Page 14: Turbidity Operation
Press down firmly to lock the Optical Application Module in place. You will hear or feel a click indicating the module has snapped into place. (See Figure 9.) 4. Close the lid and power ON the Trilogy. Use the touch screen to identify the type of Optical Application Module installed. -
Page 15: Touch Screen Basics (Turbidity )
The "Home" screen appears after confirmation of the Optical Application Module. The "Home" screen provides orientation for the multiple functions of the Trilogy. From the "Home" screen, select "Calibrate," "Tools," "Mode," or "Help." The "Home" screen is also the measurement screen. - Page 16 PC when properly connected, (see Section VII). Please note the Trilogy does not store more than 20 measurements at one time. If more than 20 readings are taken, the oldest reading will be overwritten. Measurements are not stored between power cycles.
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Page 17: Calibration Overview
Relative Fluorescence Units (RFU). Use a standard curve to determine the concentration of the analyte in the samples. The Trilogy does not manipulate data while operating in the Raw Fluorescence Mode. It is not necessary to zero the Trilogy for use in the Raw Fluorescence Mode;... -
Page 18: Direct Calibration" Procedure - A) Fluorescence And Turbidity Modules, Single Point And Multi Point Cal
E. “Direct Calibration” Procedure – a) Fluorescence and Turbidity Modules, Single Point and Multi Point Cal The following procedure applies to Trilogy and the Fluorescence or Turbidity Modules. See page 20 for procedures to calibrate the Chl Acidification and Non-Acidification Modules. - Page 19 Enter the concentration for the first Standard. If using the Turner Designs Chlorophyll a Standard, this will be the concentration data supplied with the Standard. If doing multi point calibrations, be sure to use Standards in order of increasing concentration.
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Page 20: Extracted Chlorophyll Measurements With The Chl A - Acidification Fluorescent Module
G. Calibrating and Displaying Corrected Readings for the Chl a Acidification and Non-Acidification Modules The Trilogy software can correct displayed chlorophyll readings for filtered and solvent volumes used during the calibration and measurement cycles. Be sure to touch either “Chl A” or “Chl NA”... - Page 21 Select the unit of measurement Insert calibration “blank” and touch “OK” g) Enter the concentration for the first Standard. If using the Turner Designs Chlorophyll a Standard, this will be the concentration data supplied with the Standard. If doing multi point calibrations, be sure to use Standards in order of increasing concentration.
- Page 22 If the ratio is within the required range, touch “OK”. The ratio will be stored in the Trilogy for use in the EPA Method 445.0 for measurement of chlorophyll a and pheophytin a in water. k) After the calibration is complete, either select “Proceed with Current...
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Page 23: Direct Calibration" Procedure - C) Extraction, Non-Acidification, Single Point And Multi Point
. This will provide the correlation between the solid standard and the actual chlorophyll a concentration. Information on doing a chlorophyll extraction can be found on the Turner Designs web site at this URL: http://www.turnerdesigns.com/t2/doc/appnotes/998_9000.html Trilogy Fluorometer User’s Manual... -
Page 24: Use Of The Solid Secondary Standard For Dye Applications
Turner Designs URL: http://www.turnerdesigns.com/t2/doc/appnotes/main.html#fluorescent N. Trilogy Calibration Procedure, (Absorbance Module) Calibrate the Trilogy after powering up, and after changing filters. For best results, calibrate the Trilogy and Absorbance Optical Module immediately before reading a series of samples. Instructions Sample Screen a) Turn on the Trilogy b) Touch “Absorbance”... - Page 25 When the blanking is complete, the display will revert to the Absorbance Home/ Measurement Screen. This completes the Absorbance calibration procedure, and the Trilogy is now ready to make Absorbance measurements. Trilogy Fluorometer User’s Manual P/N 998-7210 Page 25 of 38...
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Page 26: Touch Screen Basics
VI. Touch Screen Basics Touch Screen The touch screen provides a user-friendly method to operate the Trilogy. The touch screen is sensitive to the light pressure of a fingertip. It is not necessary to use a stylus. After 20 minutes without activity or user stimulation, the touch screen hibernates. -
Page 27: Systems Connections
VII. Systems Connections Establish a connection between the Trilogy and a PC or a printer to export data. Connect the 9- pin RS-232 serial cable between the Trilogy and the PC or printer. The male 9-pin connector attaches to the Trilogy and the female connector attaches to the PC. -
Page 28: Spreadsheet Interface Software Installation
COM 1, and that the data will be displayed in an EXCEL spreadsheet Excel Spreadsheet Example Turner Designs SAMPLE-001 426.73 RFU SAMPLE-002 24.57 RFU SAMPLE-003 35.49 RFU SAMPLE-004 2.56 RFU Trilogy Fluorometer User’s Manual P/N 998-7210 Page 28 of 38... -
Page 29: Appendix A - Troubleshooting
Tools menu Install the proper filter and use the ultrapure Bad Calibration water in a clean cuvette to update the zero. Error Message Check the Calibration details from the Tools menu. Trilogy Fluorometer User’s Manual P/N 998-7210 Page 29 of 38... -
Page 30: Turbidity Troubleshooting
Check the RS-232 Excel Fluorometer not connection between connected to PC. the Trilogy and the PC. New data There is an editing Wait until all the data is does not process occurring collected before editing report to Excel within an Excel cell. - Page 31 IT support desk. Log in as Administrator The software was not The software and Remove the installed properly. does not open software and re-install. Trilogy Fluorometer User’s Manual P/N 998-7210 Page 31 of 38...
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Page 32: Appendix B - Warranty And Obtaining Service
Appendix B - Warranty and Obtaining Service Warranty Turner Designs warrants the Trilogy and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions: •... -
Page 33: Appendix C - Specifications
50-Well Storage Box Solid Secondary Standard 100 to 240VAC Universal Power Power Supply Supply included, Output 12VDC 12 mm Round Vial Adaptor 0.84A Max CD with User’s Guide and Spreadsheet Interface Software Trilogy Fluorometer User’s Manual P/N 998-7210 Page 33 of 38... -
Page 34: Appendix D - Principles Of Fluorescence
The lamp actually provides a broader range of light than that which excites the compound. This broad light range is illustrated by the "many wavelengths of light" shown in Figure 13. Trilogy Fluorometer User’s Manual P/N 998-7210 Page 34 of 38... -
Page 35: Appendix E - Recommended Measurement Practices
Practices Linear Range and Quenching The linear range is the concentration range in which the Trilogy output is directly proportional to the concentration of the fluorophore. The linear range begins with the smallest detectable concentration, and spans to an upper limit (concentration) that is dependent upon: the properties of the fluorescent material;... -
Page 36: Appendix F - Chlorophyll A Acidification And Non-Acidification Calculations
Non Acidification Method I. End calculation for corrected chlorophyll a Chlorophyll a concentration = C * [(F )/ (F )] * (V stand[1] samp blank stand[1] blank solvent water Trilogy Fluorometer User’s Manual P/N 998-7210 Page 36 of 38... -
Page 37: Appendix G - In Vivo Fluorescence Measurements Using Trilogy
This will provide the correlation between the solid standard and the actual chlorophyll a concentration The solid secondary standard can now be used to recalibrate and/or check the stability of the Trilogy fluorometer. Trilogy Fluorometer User’s Manual... - Page 38 Turner Designs 845 W. Maude Ave. Sunnyvale, CA 94085 Phone: (408) 749-0994 FAX: (408) 749-0998 www.turnerdesigns.com Trilogy Fluorometer User’s Manual P/N 998-7210 Page 38 of 38...
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