Related Manuals for Turner Designs AquaFluor
Summary of Contents for Turner Designs AquaFluor
- Page 1 ® AquaFluor Handheld Fluorometer and Turbidimeter December 15, 2015 P/N: 998-0851 Revision 1.7 TURNER DESIGNS 845 W. Maude Ave. Sunnyvale, CA 94085 Phone: (408) 749-0994 FAX: (408) 749-0998...
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Page 2: Table Of Contents
Data Quality Applications In Vivo Chlorophyll Extracted Chlorophyll a Ammonium Detection Turbidity Fluorescent Tracer Dyes Cyanobacteria Monitoring CDOM/FDOM Optical Brighteners Warranty Terms Warranty Service Out of Warranty Service Appendixes Specifications Linear Range, Quenching and Temperature Considerations User’s Manual ® AquaFluor... - Page 3 WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well-being of our environment. Accordingly, we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products.
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Page 4: Introduction
The AquaFluor is a lightweight, handheld fluorometer/turbidimeter ideal for field use. Its ® small size and durability make the AquaFluor perfect for the user who needs quick measurements away from the laboratory. Dual-channel capability allows users to measure fluorescence and turbidity of one sample. -
Page 5: Setup
Do not force oversized cuvettes into the sample compartment. This can damage ® the sample compartment. If the cuvette does not easily fit down inside the AquaFluor you will need to purchase different cuvettes. Round glass test tubes can be used with an optional adapter that is available. Refer to Appendix A for cuvette information. -
Page 6: Instrument Operation And Calibration
Ideally the Primary Standard and Blank samples used for calibrating will be made with the same water the tests are being performed in. For more details on this and tracer dye use, please refer to the Turner Designs website under Application Notes for “A Practical Guide to Flow Measurement”, document 998-5000. -
Page 7: Calibration Procedure
1. Press the <CAL> button. 2. Press <ENT> to start the calibration. ® 3. Insert your blank sample and press <ENT>. The AquaFluor will average the reading for 10 seconds and set the blanking zero point. 4. Insert the standard sample and press <ENT>. The reading is averaged for 10 seconds and the Standard Calibration value is set. -
Page 8: Calibration Check
Please refer to Appendix A for the solid standard configuration for your application. ® When a Secondary Standard is used, the AquaFluor will give relative sample readings that are proportional to the measured fluorescence. In some cases these relative sample readings will be correlated back to actual concentrations that are determined later. - Page 9 Photo 2. Placing the adjustable secondary standard in the AquaFluor™ 4. Wait approximately 10 seconds while the instrument averages the reading, then read the value. 5. You can use the 3/32” Allen Wrench provided to adjust the attenuation screw through the hole at the top of the secondary standard to increase or decrease the value displayed on the screen.
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Page 10: Sample Analysis
® secondary standard to verify the AquaFluor calibration. If the secondary standard value ® has changed by more than ± 5% of its assigned value, the AquaFluor should be recalibrated. NOTE: Recalibration with a primary standard if possible is recommended. -
Page 11: Data Transfer And Internal Data Logging (Idl)
USB to Serial Adaptor Cable. ® Plug the opposite end of the cable into the port at the base of the AquaFluor Open the Spreadsheet Interface software. Click on the box to the right of the COM port icon to select the appropriate COM port. -
Page 12: Data Transfer Troubleshooting
® Connect the AquaFluor to the serial port of your computer. Open the Turner Designs Interface Software. See Section 3.1-3.3 for computer requirements and installation. Press the <DATA> button 3 times. Press <ENT> 5 times to start the data download. -
Page 13: Sample Analysis Guidelines
® The AquaFluor is very sensitive and even small amounts of material from a previous sample may contaminate the sample and result in errors. Use a clean cuvette for all readings. -
Page 14: Applications
However, in most cases distilled water is used for the Blank sample since the in vivo readings are semi-quantitative at best. Graph 1 For more details on chlorophyll analysis visit Turner Designs’ website under Applications. User’s Manual ®... -
Page 15: Extracted Chlorophyll A
Chlorophyll standard P/N 10-850 is used for calibration and the measurements are made using the 12 x 75 mm glass test tubes. EPA Method 445.0 is a popular Chlorophyll extractive method that was developed using a Turner Designs Model 10 fluorometer and is published by the United States Environmental Protection Agency. ®... -
Page 16: Turbidity
The two most commonly used tracer dyes are Fluorescein and Rhodamine WT. The ® Fluorescein dye can be detected using the Blue channel on the AquaFluor and Rhodamine dye is detected using the Green channel. The linear detection range for both dyes is 0.4 to 400 ppb (active ingredient) in potable water. -
Page 17: Cdom/Fdom
For more information on CDOM / FDOM visit Turner Designs website under Applications. 5.8 Optical Brighteners Optical Brightener Agents (OBAs) are primarily added to laundry soaps, detergents, and cleaning agents for the purpose of brightening fabrics and/or surfaces. -
Page 18: Terms
(RMA). Package the unit, write the RMA number on the outside of the shipping carton, and ship the instrument, prepaid, to Turner Designs. If the failure is covered under the warranty terms, the instrument will be repaired and returned free of charge, for all customers in the contiguous continental United States. -
Page 19: Out Of Warranty Service
Shipment to Turner Designs should be prepaid. Your bill will include return shipment freight charges. Address for Shipment: Turner Designs 845 W. Maude Avenue Sunnyvale, CA 94085 User’s Manual... - Page 20 User’s Manual ® AquaFluor...
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Page 21: Appendixes A Specifications
Appendix A: Specifications ® AquaFluor 1.75” x 3.5” x 7.25” Size (4.45cm x 8.9cm x 18.4cm) Weight 13.9oz (0.4kg) Resolution 12 bits LCD Display 2 x 16 characters Case Meets IP 67 Standard; dustproof and waterproof Temperature 41-104 °F; 5-40 °C... - Page 22 Adapter P/N 8000-936 and Minicells (500 ct.) P/N 138-0140 are included with Ammonium Channel. **Readings on the Phycocyanin channel using cuvettes or vials have a maximum range of approximately 3,500 ppb. Minicells P/N 7000-950 and Minicell adaptor P/N 8000-936 are required to achieve maximum range of approximately 20,000 ppb. User’s Manual ® AquaFluor...
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Page 23: B Linear Range, Quenching And Temperature Considerations
Appendix B: Linear Range, Quenching and Temperature Considerations ® The linear range is the concentration range in which the readout of the AquaFluor is directly proportional to the concentration of the fluorophore in the sample. The linear range begins with the lowest detectable concentration and spans to an upper limit concentration that is dependent on the properties of the fluorescent material and the cuvette optical path length.