Thermo Scientific 7452 Operating And Maintenance Manual
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Thermo Scientific 7452 Operating And Maintenance Manual

7452 series. cryomed controlled rate freezer
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Model 7452 Series
CryoMed Controlled Rate Freezer
Operating and Maintenance Manual 7007452 Rev. 11

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  • Page 1 Model 7452 Series CryoMed Controlled Rate Freezer Operating and Maintenance Manual 7007452 Rev. 11...
  • Page 2 7456 2. A PDF version of the Windows Help file for the PC Interface 7451 7457 Software is located on the CD-ROM. The file name is 7452 7458 “Help_PC_Interface.pdf ”. 7453 7459 3. A “Read Me” file containing the known issues with Version 1.0 of the...
  • Page 3 Material in this manual is for information purposes only. The contents and the product it describes are subject to change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of this manual.
  • Page 4 This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific has contracted with one or more recycling/disposal companies in each EU Member State European Country, and this product should be disposed of or recycled through them.

  • Page 5: Section 9 Troubleshooting

    We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service...

  • Page 6: Table Of Contents

    Holder Adjustment ........2-21 Thermo Scientific...
  • Page 7 Saving Changes ........10-26 Sharing Host PC Resources ......10-26 7007452 CryoMed Freezer Thermo Scientific...

  • Page 8: Introduction

    Introduction Section 1 Figure 1-1. Non-IVF Controlled Rate Freezer Figure 1-2. IVF Controlled Rate Freezer (FDA clearance for human research) Thermo Scientific 7007452 CryoMed Freezer...

  • Page 9: Standard Features

    Displays There are three displays on the Controlled Rate Freezer control panel: Chamber and Sample temperature, ranging from +54° to -199°C, the LCD Display for profile status, messages, and freezing profile editing and viewing. 7007452 CryoMed Freezer Thermo Scientific...

  • Page 10: Indicators

    Graphs and cycle data, as well as additional information, are printed out at the top of the unit on a thermal printer in real-time mode. A connector is also available on the back of the unit for an optional strip chart temperature recorder. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 11: Pre-Set Profiles

    Step 4 ....... . .End 7007452 CryoMed Freezer Thermo Scientific...
  • Page 12 Step 7 ....... . .End Thermo Scientific 7007452 CryoMed Freezer...
  • Page 13 Step 7 ....... . .End S = Sample Temperature C = Chamber Temperature C/m = degrees Centigrade per Minute 7007452 CryoMed Freezer Thermo Scientific...

  • Page 14: Set-Up And Operation

    When closing the freezer door, be sure to turn the handle 180° in rotation to fully seal the door. Figure 2-1. Connections on Unit Back Thermo Scientific 7007452 CryoMed Freezer...
  • Page 15 Section 2 Set-Up and Operation Figure 2-2. Clearance for All Units 7007452 CryoMed Freezer Thermo Scientific...

  • Page 16: Supplies

    2 Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 17: Sample Thermocouple Connection

    4. To choose a profile, scroll through the selections using the up/down arrow keys. The profiles are displayed in a loop. Pressing either of the arrow keys consecutively brings the list back to the starting point. 7007452 CryoMed Freezer Thermo Scientific...

  • Page 18: Access Code Security

    5. Press the Run key. If the chosen profile begins with a Wait, the Run key must be pressed again to continue, after the appropriate temperature is reached. 6. The mode indicator is lit, showing the status of the profile. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 19: Stop A Profile Run

    However, Access Code protection is not required on an End step. If a power outage interrupts a run, the profile will restart from the point of interruption when power is restored. See Alarms in this section. 7007452 CryoMed Freezer Thermo Scientific...

  • Page 20: Manual Override

    Move the profile (USER 1 through USER 10) that you wish to view or edit to the top line by pressing the 5or 6 keys. Note Pre-set Profiles may not be edited. ▲ 2. To enter View Mode, press the Enter key. Thermo Scientific 7007452 CryoMed Freezer...
  • Page 21 Step 1 contains a command with a cursor blinking just to the right of a left pointing arrow (← __ ). With the cursor in this position, use the up or down arrow keys to scroll through the available commands. 7007452 CryoMed Freezer Thermo Scientific...
  • Page 22 For example, if the chamber set point is 30°C, the controller will ramp to 31°C until the setpoint is reached. Graph print-outs show this ramp Figure 2-4. Ramp Settings setting. ▲ Thermo Scientific 7007452 CryoMed Freezer...

  • Page 23: Copy And Paste Pre-Set And User Profiles

    When the desired User Profile is showing on the first line of the display, press the Enter key to complete the paste operation, or press Back to cancel. 2-10 7007452 CryoMed Freezer Thermo Scientific...

  • Page 24: Recorder

    Run start time and date Profile name 1234 cell culture media User information D.M.S.O. Time/Temp scale Chamber temp Sample temp Profile steps listed as they occur Temp tracking alarm Profile line Figure 2-4. Print-outs Thermo Scientific 7007452 CryoMed Freezer 2-11...

  • Page 25: Alarms

    Alarm LED and clear the alarm message from the fourth line of the display. The numeric display message will revert to a normal temperature display when the alarm condition clears. 2-12 7007452 CryoMed Freezer Thermo Scientific...

  • Page 26: Sample Thermocouple Open

    Immediately switches to Idle Mode, • Disables all control functions. Pressing the Silence key will mute the audible alarm, however, the Alarm LED and display message will remain active until the probe condition is resolved. Thermo Scientific 7007452 CryoMed Freezer 2-13...

  • Page 27: Heater Malfunction

    The audible alarm is silenced by pressing the Silence key. Since this alarm indicates a hard failure, the Alarm LED and fourth line message can only be cleared by turning the unit power off, then on again. 2-14 7007452 CryoMed Freezer Thermo Scientific...

  • Page 28: Chamber/Sample Over/Under Limit Temp

    However, the alarm message remains on the fourth display line until the Silence key is depressed. If the temperature of either probe continuously exceeds the pre-set limit temperatures for more than 10 minutes, the unit will immediately switch to Idle Mode and disable all functions. Thermo Scientific 7007452 CryoMed Freezer 2-15...
  • Page 29 Alarm LED will stop flashing and the audible alarm will silence (if the Silence key has not already been pressed). However, the alarm message will remain on the fourth display line until the Silence key is pressed a second time. 2-16 7007452 CryoMed Freezer Thermo Scientific...

  • Page 30: Power Failure

    The controller will maintain the temperature of the final step in the profile run until the run is stopped by the operator pressing the Back key. Caution If performing successive runs, dry the gaskets and heat breaks between each run. ▲ Thermo Scientific 7007452 CryoMed Freezer 2-17...

  • Page 31: Warm Complete

    Note: The printer continues to print current Run data even though the ‘Printer Buffer Full’ alarm has occurred. IVF Sample Holders Included with the IVF Controlled Rate Freezer are a straw holder, vial holder, cane holder and access port plugs. 2-18 7007452 CryoMed Freezer Thermo Scientific...

  • Page 32: Using A Vial Holder

    4. To install the straw probe, insert the probe down the center of the button indicated below. Slide the probe down through the holder until it feeds through the center straw. Thermo Scientific 7007452 CryoMed Freezer 2-19...

  • Page 33: Using The Cane Holder

    If the cane holder is used with a sample probe, use the standard 1.2ml vial probe. Connect the probe to the internal sample probe connection. Caution Do not connect two sample probes at the same time. ▲ 2-20 7007452 CryoMed Freezer Thermo Scientific...

  • Page 34: Holder Adjustment

    3. Tighten the small hex socket set screws when the adjustment is complete, just until the screws lightly touch the rods to avoid damaging the rod threads. Figure 2-7. Vial Holder Adjustment Figure 2-8. Straw Holder Thermo Scientific 7007452 CryoMed Freezer 2-21...

  • Page 36: Configuration/Calibration

    Enter and Back keys, and changing the digit using the up/down arrow keys. The default Access Code (security disabled) is 0 0 0 0. Each digit may be set over a range of 0 to 9. Exit this menu by using the Back key. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 37: Section 3 Configuration/Calibration

    Selects the thermal printer operating mode, printer scale, or initiates printing of the previous run chart. Select the desired item using the Enter and Back keys and change the selected parameter using the 5/6 keys. Exit the menu using the Back key. 7007452 CryoMed Freezer Thermo Scientific...
  • Page 38 The Chamber and Sample probe offset can be adjusted over a range of -9.9°C to +9.9°C in 0.1°C increments. Adjust the probe offset for a 0.0°C temperature display reading with the probe in a standard crushed ice bath. Thermo Scientific 7007452 CryoMed Freezer...
  • Page 39 7. Repeat Steps 3 through 6 as necessary until both temperature displays read 0.0°C and -196°C. There may be some interaction between the ‘Offset’ and the ‘Scaler’ adjustments. 7007452 CryoMed Freezer Thermo Scientific...
  • Page 40 Heater error • Check LN2 supply • RS-232 communication error C. Reset Error Logs This menu item is used to clear the Error Log entries. When the log is empty, this item reads “Error Log Empty”. Thermo Scientific 7007452 CryoMed Freezer...
  • Page 41 P.I.D. parameters for the heater vs. LN2 injection cooling. Exit this menu using the Back key. There is a separate set of parameters for each size of chamber available ([L] 1.7 cu ft, [M] 1.2 cu ft, and [S] 0.6 cu ft). 7007452 CryoMed Freezer Thermo Scientific...
  • Page 42 Simulation (Off; Cool: 1S, 10S, 90C/m; Heat: 1S, 45S, 9C/m) • Thermal Controller (Size M, 1000mS, P: 0.25, I: 0.075, D: 0.2, PR: 0.01, CP: 4, CM: 1.0, C) • Tracking Alarms (5C, 1m) Note User Profiles are not affected. ▲ Thermo Scientific 7007452 CryoMed Freezer...
  • Page 43 (Time - 1 minute increments) and temperature difference from setpoint (Delta - 1°C increments). The Time parameter can be adjusted over a range from a 1 to 99 minute range. Exit the menu using the Back key. 7007452 CryoMed Freezer Thermo Scientific...

  • Page 44: Maintenance

    After the cabinet has been rinsed, spray with 70% alcohol. 4. Allow the cabinet door to remain open for the alcohol to evaporate. 5. Plug the freezer in and turn the power switch on. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 45: Clean A Major Spill In The Chamber

    9. Connect the Sample probe to the connector, if applicable. 10. All chamber parts must be re-installed for consistent uniformity and airflow. Connect the unit to the power source and turn it on. 11. The freezer is ready to be returned to service. 7007452 CryoMed Freezer Thermo Scientific...

  • Page 46: Clean The Cabinet Exterior

    ▲ Clean Gasket/Heat Dry any moisture from the door gasket and heat breaks between each run, using a soft cloth, to prevent ice build-up which could reduce the Break effectiveness of the door seal. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 48: Printer Set-Up

    4 inches (10cm) through the paper cutter. 5. Press the paper feed switch until the paper Insertion feeds in straight and smoothly. Slot The printing surface is the outside of the thermal paper roll. Thermo Scientific 7007452 CryoMed Freezer...

  • Page 49: Section 5 Printer Set-Up

    Odd (ON) 4: Busy control Hardware (ON) XON/XOFF (OFF) 5: Baud rate (a) 75bps (ON) 9600bps (OFF) 6: Baud rate (b) (ON) (ON) 7: Baud rate (c) (ON) (ON) 8: Baud rate (d) (ON) (ON) 7007452 CryoMed Freezer Thermo Scientific...

  • Page 50: Specifications

    Rack and Holder Vial Placement for each size chamber 0.6 cu. ft. 1.2 cu. ft. 1.7 cu. ft. Models Models Models 7450/7451 7452/7453 7454/7455 7456/7457 7458/7459 7474 7472, 7475 7473, 7476 Thermo Scientific 7007452 CryoMed Freezer...

  • Page 51: Section 6 Specifications

    2) 2.0C/min C to 0.0C 7450 140 (63.5 ) 3) 0.0C/min S to 3.0C 7451 140 (63.5) 4) Hold 10.0min @1.0C 7452 160 (72.6) 7453 160 (72.6) 5) 1.0C/min C to -5.0C 7454 180 (81.6) 6) 99C/min C to -50C 7455 180 (81.6)
  • Page 52 External Connections Power entry Strip chart temperature recorder PC interface port LN2 connection 1/2” flare External thermocouple jack (IVF models only) Sample access (IVF models only) Thermo Scientific 7007452 CryoMed Freezer...

  • Page 54: Parts List

    270159 DB25F to DB9M Adapter 370248 T/C Plug Type T 8028 Flatbed Chart Recorder 370249 T/C Socket Type T 4000404 PC Interface Software 4000385 Internal Sample Probe 1.2 ml 4000386 Internal Sample Probe 4/5 ml Thermo Scientific 7007452 CryoMed Freezer...
  • Page 55 Cane, 1.2 ml, 6 ampule 180254 Cane Holder Plug 4000402 External Straw Sample Probe 4000403 External Vial Sample Probe 4000712 Sample T/C Vial with hole, 1.2 ml 4000713 Sample T/C Vial with hole, 2.0 ml 7007452 CryoMed Freezer Thermo Scientific...
  • Page 56 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer...
  • Page 57 Section 7 Parts List 7007452 CryoMed Freezer Thermo Scientific...
  • Page 58 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer...
  • Page 59 Section 7 Parts List 7007452 CryoMed Freezer Thermo Scientific...
  • Page 60 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer...
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  • Page 62 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer...
  • Page 63 Section 7 Parts List 7-10 7007452 CryoMed Freezer Thermo Scientific...
  • Page 64 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer 7-11...
  • Page 65 Section 7 Parts List 7-12 7007452 CryoMed Freezer Thermo Scientific...
  • Page 66 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer 7-13...
  • Page 67 Section 7 Parts List 7-14 7007452 CryoMed Freezer Thermo Scientific...
  • Page 68 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer 7-15...
  • Page 69 Section 7 Parts List 7-16 7007452 CryoMed Freezer Thermo Scientific...
  • Page 70 Section 7 Parts List Thermo Scientific 7007452 CryoMed Freezer 7-17...
  • Page 71 Section 7 Parts List 7-18 7007452 CryoMed Freezer Thermo Scientific...
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  • Page 84: Pc Interface Software

    9-pin RS232 ports (1 communication port for each freezer) • Support baud rates up to 9600K baud minimum • 16550 UARTS (suggested) • 128 byte transmit and receive buffers (FIFOs, suggested) • Automatic assignment of ports (suggested) Thermo Scientific 7007452 CryoMed Freezer 10-1...

  • Page 85: Installation And Set-Up

    Mission statement. Click anywhere on this screen to close it. • The Exit button closes the PC interface application software. All other windows must be closed before exiting to avoid error messages. 10-2 7007452 CryoMed Freezer Thermo Scientific...

  • Page 86: Edit Screen

    "Program waiting for user -- Click here to continue" button on the Run screen. The temperature can be set over a range of -180°C to +50°C with a setting resolution of 0.1ºC. Thermo Scientific 7007452 CryoMed Freezer 10-3...
  • Page 87 This step can only be used when the starting Sample temp is greater than the specified Sample temp (the Sample is cooling). Note The sample temp must be greater than the chamber temp. ▲ 10-4 7007452 CryoMed Freezer Thermo Scientific...
  • Page 88 Note The slope of the temperature ramp is determined automatically, based on the temperature entered and the chamber temperature at the beginning of the step. Entry of a sign for the rate term is not required/allowed. ▲ Thermo Scientific 7007452 CryoMed Freezer 10-5...
  • Page 89 X is the step number Automatic advance to a subsequent step or return to a previous step in a freezing profile. The Jump function can be programmed over a range of Step 1 to Step 20. 10-6 7007452 CryoMed Freezer Thermo Scientific...
  • Page 90 3. END is the mandatory last step. It alerts the freezer that the profile steps are completed. This step is automatically added during the profile creation. It cannot be deleted or replaced. Thermo Scientific 7007452 CryoMed Freezer 10-7...

  • Page 91: Create A New Freezer Profile

    To choose a different default printer, first exit the interface software. Choose the printer, then restart the PC interface software. The printer selection will not be changed if the software application is still open at the time of the change. 10-8 7007452 CryoMed Freezer Thermo Scientific...

  • Page 92: Display Profile Temp Graph

    When saving a profile which has the same name as one already existing, a file overwrite error message will appear. Choose YES to overwrite (replace) or NO to enter a new file name. Thermo Scientific 7007452 CryoMed Freezer 10-9...

  • Page 93: Run Screen

    Chamber temperature, Profile, etc). If 'Color' is selected with a monochrome printer, colors are represented by shades of gray so some colors may not be clearly visible. c. Selecting 'Single page report' prints the current Run screen. 10-10 7007452 CryoMed Freezer Thermo Scientific...
  • Page 94 This button is only available during a profile Run. a. The override Heat button turns the heater on. b. The override Cool button turns the Cool solenoid on. c. The override Cool+ button turns the Cool and Cool+ solenoids Thermo Scientific 7007452 CryoMed Freezer 10-11...
  • Page 95 Scroll the Graph from side-to-side, using the right/left arrow buttons. Use the up/down arrow buttons to scroll the graph top-to-bottom. Turn Auto-Scroll off to view a portion of the graph off-screen from the current plot position. 10-12 7007452 CryoMed Freezer Thermo Scientific...

  • Page 96: Status Indicators

    Alarm messages may also appear before or during a run. These messages alert the user to the alarm status of the freezer. The user must clear the message on the screen even though the alarm condition may have already corrected itself. Thermo Scientific 7007452 CryoMed Freezer 10-13...
  • Page 97 Back button at the freezer while the profile is in the End step. Click on the message to acknowledge that the run was stopped. This action has the same effect as clicking the Stop button. 10-14 7007452 CryoMed Freezer Thermo Scientific...

  • Page 98: Typical Run Procedure

    End step. The Run may be stopped by clicking on the Stop button or pressing the Back key on the freezer keypad. 10. Enter any additional comments when the text entry window opens again. This window appears regardless of the Run stop method. Thermo Scientific 7007452 CryoMed Freezer 10-15...

  • Page 99: Power Failure Recovery

    The graph on the Run screen will show a temperature occurrence of zero to indicate a freezer power failure, rather than a PC-only power failure. A communication error message will display on the Run screen during the freezer power failure. 10-16 7007452 CryoMed Freezer Thermo Scientific...

  • Page 100: Error Messages

    In this case, the PC cannot take control. Select the ‘Terminate Session’ button to close the Run window. Thermo Scientific 7007452 CryoMed Freezer 10-17...

  • Page 101: Remote Mode

    The message 'Under Remote Control' displays on the freezer as well as the current profile step being executed. Alarm messages alternate with the current step information if an alarm condition occurs. 10-18 7007452 CryoMed Freezer Thermo Scientific...

  • Page 102: Stand Alone Mode

    Review step select pull-down menu: Displays the steps of the profile in the open ‘Review’ Run file • Compare step select pull-down menu: Displays the steps of the profile in the open ‘Compare’ Run file Thermo Scientific 7007452 CryoMed Freezer 10-19...

  • Page 103: User Controls

    ‘monochrome’. If color, select either. Monochrome uses marker/line patterns to identify specific lines on the graph. If ‘Color’ is selected with a monochrome printer, colors are represented by shades of grey (some colors may not be clearly visible). 10-20 7007452 CryoMed Freezer Thermo Scientific...
  • Page 104 1) Compare file Profile name and path 2) Compare Run data file and path 3) User name 4) Date/time 5) User Information Field name/contents 6) Run start/stop "comments" 7) Profile listing 8) Alarm listing with time of occurrence Thermo Scientific 7007452 CryoMed Freezer 10-21...
  • Page 105 • Graph Scroll Buttons The left/right scroll arrow buttons move the graph right or left in the display window. The up/down scroll arrow buttons move the graph up or down in the display window. 10-22 7007452 CryoMed Freezer Thermo Scientific...

  • Page 106: Options

    Click the palette button to create custom colors. When the cursor is over the desired color, click the left mouse button. Click Save to keep changes. Click Restore to return to previously saved colors. Click Exit to exit Options menu. Thermo Scientific 7007452 CryoMed Freezer 10-23...

  • Page 107: Information Field Set-Up

    Enter the desired path in the window for the default program directory and run data directory. A Windows directory browse button may also be selected. Click Save to keep changes. Click Restore to return to previously saved changes. Click Exit to exit Options screen. 10-24 7007452 CryoMed Freezer Thermo Scientific...

  • Page 108: User Name And Password Maintenance

    If no user names are defined, all menus can be accessed without password protection. Anonymous is listed as the user name if no users are set up. If password protection is no longer desired, simply delete all user names. Thermo Scientific 7007452 CryoMed Freezer 10-25...

  • Page 109: Saving Changes

    Run. Run data will be lost during the time of the communication interruption. Experiment with test runs, or if in doubt, don't run other applications during a critical profile run. 10-26 7007452 CryoMed Freezer Thermo Scientific...
  • Page 110 Thermo Scientific 7007452 CryoMed Freezer 11-1...
  • Page 111 11-2 7007452 CryoMed Freezer Thermo Scientific...
  • Page 112 Thermo Scientific 7007452 CryoMed Freezer 11-3...
  • Page 113 11-4 7007452 CryoMed Freezer Thermo Scientific...
  • Page 114 Thermo Scientific 7007452 CryoMed Freezer AA-1...
  • Page 115 AA-2 7007452 CryoMed Freezer Thermo Scientific...
  • Page 116 Thermo Scientific 7007452 CryoMed Freezer AA-3...
  • Page 117 AA-4 7007452 CryoMed Freezer Thermo Scientific...
  • Page 118 Note Because argon is an inert gas whose physical properties are very similar to those of nitrogen, the precautions and safe practices for handling and use of liquid argon are the same as those for liquid nitrogen. ▲ Thermo Scientific 7007452 CryoMed Freezer AA-5...
  • Page 119 Rapid warming of the affected part is best achieved by using water at 108°F. Under no circumstance should the water be over 112°F, nor should the frozen part be rubbed either before or after rewarming. The patient should neither smoke nor drink alcohol. AA-6 7007452 CryoMed Freezer Thermo Scientific...
  • Page 120 Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Marietta, Ohio 45750 United States www.thermo.com...

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