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Summary of Contents for Thermo Scientific BIOShield 720
- Page 1 Thermo Scientific BIOShield™ 720 Instruction Manual 50121034-d • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
- Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
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Page 5: Table Of Contents
Chapter 6 Maintenance and Care ......................6-1 Cleaning intervals ....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination ..................... 6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-6 RCF-Values .......................A-1 Chemical Compatibility Chart ................B-1 Thermo Scientific 75003621 BIOShield... -
Page 7: Preface
• Do not use rotors which show any signs of corrosion and / or cracks. • Use only with rotors which have been loaded properly. • Never overload the rotor. • Never run the rotor without lid. Thermo Scientific 75003621 BIOShield... - Page 8 ATTENTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. 75003621 BIOShield Thermo Scientific...
- Page 9 Rotor Specifications Contents “Technical Data” page 1-2 • Thermo Scientific 75003621 BIOShield...
- Page 10 Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7 Pitch angle [°] Accel. / braking time [s] 65 / 65 45 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003621 BIOShield Thermo Scientific...
- Page 11 Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7 Pitch angle [°] Accel. / braking time [s] 75 / 70 55 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003621 BIOShield...
- Page 12 Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7 Pitch angle [°] Accel. / braking time [s] 65 / 65 45 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003621 BIOShield Thermo Scientific...
- Page 13 Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7 Pitch angle [°] Accel. / braking time [s] 75 / 70 55 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003621 BIOShield...
- Page 14 Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7 Pitch angle [°] Accel. / braking time [s] 75 / 70 55 / 50 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003621 BIOShield Thermo Scientific...
- Page 15 Rotor Specifications Technical Data Table 1-11. 230 V, 50 / 60 Hz ventilated Thermo Scientific SL 16 Centrifuge Catalog # 75004000 Weight of empty rotor [kg] Max. cycle number 66,000 Maximum permissible load [g] 4 x 470 Maximum speed n...
- Page 16 Rotor Specifications Technical Data Table 1-13. 120 V, 60 Hz ventilated Thermo Scientific SL 16 Centrifuge Catalog # 75004001 Weight of empty rotor [kg] Max. cycle number 66,000 Maximum permissible load [g] 4 x 470 Maximum speed n [rpm] 5,300...
- Page 17 Accessories Contents “Rotor data” • page 2-2 “Accessories” page 2-2 • Thermo Scientific 75003621 BIOShield...
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Page 18: Chapter 1 Rotor Specifications
Radius (cm) RCF/Radius 71880 16.2 Maximum 29700 Minimum BIOShield 720 Rotor Package Cat. No. Description 75003621 BIOShield 720 with buckets and sealing lid Tube Ordering Information Sealing Assembly Required: Adapters Required: Accessories Required: Tube Vol. Max. Dims ØxL Cat. No. Vol. - Page 19 ™ AutoLock Contents “Rotor Installation” page 3-2 • • “Removing the Rotor” page 3-3 Thermo Scientific 75003621 BIOShield...
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Page 20: Chapter 3 Autolock
WARNING If the rotor cannot be properly locked in place after several attempts, then the ™ AutoLock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor, damaged rotors must not be used. Keep the hub area clear of objects. 75003621 BIOShield Thermo Scientific... -
Page 21: Removing The Rotor
2. Grab the rotor handle with both hands and press against the green AutoLock button. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this. Thermo Scientific 75003621 BIOShield... -
Page 23: Rotor Loading
Rotor Loading Contents “Before a Run” page 4-2 • • “Proper Loading” page 4-2 “Improper Loading” page 4-3 • • “Maximum Loading” page 4-3 “Cycle Counter” page 4-4 • Thermo Scientific 75003621 BIOShield... -
Page 24: Before A Run
Always be sure to use buckets of the same weight class, which is marked on the buckets themselves. Proper Loading To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times. 75003621 BIOShield Thermo Scientific... -
Page 25: Improper Loading
There are two options available for centrifuging samples whose weight, including adapter, exceeds the maximum permissible load: • Reduce the fill level. • Reduce the speed. Use the table or the formula: Actual Load (g/bucket) 6300 6170 6048 5933 5824 5721 5623 5530 5442 5357 5277 5200 5126 5055 Thermo Scientific 75003621 BIOShield... -
Page 26: Cycle Counter
WARNING Replace the rotor when the specified number of cycles is reached. Due to the mechanical load, a rotor can break and thus damage the centrifuge. Service life examples Usage profile Maximum service life at 30,000 cycles frequent use 7 years 20 runs / day 220 days / year 75003621 BIOShield Thermo Scientific... -
Page 27: Aerosol-Tight Applications
Aerosol-tight Applications Contents “Basic Principles” page 5-2 • • “Fill Level” page 5-2 “Checking the Aerosol-Tightness” page 5-2 • Thermo Scientific 75003621 BIOShield... -
Page 28: Basic Principles
Check as needed to make sure your rotor is aerosol-tight. The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks, scratches and embrittlement are extremely important. Aerosol-tight applications are not possible if the lids are open. 75003621 BIOShield Thermo Scientific... - Page 29 Damaged or clouded rotor covers are to be replaced immediately! CAUTION This quick test is not suited for validating the aerosol tightness of your ™ BIOShield 720. Check the seals and sealing surfaces of the lid thoroughly. Thermo Scientific 75003621 BIOShield...
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Page 31: Maintenance And Care
Maintenance and Care Contents “Cleaning intervals” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-3 • • “Decontamination” page 6-4 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-6 Thermo Scientific 75003621 BIOShield... -
Page 32: Cleaning Intervals
• Store the aluminium parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 75003621 BIOShield Thermo Scientific... -
Page 33: Disinfection
CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific 75003621 BIOShield... -
Page 34: Decontamination
For general radioactive decontamination use a solution of equal parts of 70 % ethanol, 10 % SDS and water. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 75003621 BIOShield Thermo Scientific... -
Page 35: Autoclaving
Place the rotor on a flat surface. Note No chemical additives are permitted in the steam. CAUTION Never exceed the permitted temperature and duration when autoclaving. If the rotor shows signs of corrosion or wear, it must be replaced. Thermo Scientific 75003621 BIOShield... -
Page 36: Service Of Thermo Fisher Scientific
• the suitability of the set-up site; • the lid lock and the safety system; • the rotor; • the fixation of the rotor and the drive shaft. Thermo Fisher Scientific offers inspection and service contracts for this work. 75003621 BIOShield Thermo Scientific... -
Page 37: Rcf-Values
16.2 1700 16.2 1800 16.2 1900 16.2 2000 16.2 2100 16.2 2200 16.2 2300 16.2 2400 16.2 1043 2500 16.2 1132 2600 16.2 1224 2700 16.2 1320 2800 16.2 1420 2900 16.2 1523 3000 16.2 1630 Thermo Scientific 75003621 BIOShield... - Page 38 5500 16.2 2266 5479 5600 16.2 2349 5680 5700 16.2 2434 5884 5800 16.2 2520 6093 5900 16.2 2607 6305 6000 16.2 2697 6520 6100 16.2 2787 6739 6200 16.2 2879 6962 6300 16.2 2973 7188 75003621 BIOShield Thermo Scientific...
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Page 39: Chemical Compatibility Chart
S U U U U U U U - M S M - Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Thermo Scientific 75003621 BIOShield... - Page 40 S S S U S U S S S U ® M S S - S S S - S S S S - S S - S S S S S S S M S S S Ficoll-Hypaque 75003621 BIOShield Thermo Scientific...
- Page 41 S S S U S S M S S S S S U S U M S S S S S S S M S M U U U U U M S S U S U U S U U U U U M U U U S S M U S U Methylene Chloride Thermo Scientific 75003621 BIOShield...
- Page 42 S S S S S S S S S S S S S S S S S S S S S U - S M S S S M S U S S S U S S S S U S U M S M S Picric Acid 75003621 BIOShield Thermo Scientific...
- Page 43 S S S S S S S S S S S S S S S S S S S S S S S M S S M S S M S S S S S S S S S M S S S S S S S S S S Citric Acid (10 %) Polyethyleneterephthalate Thermo Scientific 75003621 BIOShield...
- Page 44 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. Because, no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. 75003621 BIOShield Thermo Scientific...
- Page 45 Cycle Counter ..........6-4 Decontamination ............6-3 Disinfection ............5-2 Fill Level ..........4-3 Improper Loading ............6-1 Maintenance ..........4-3 Maximum Loading ............ 5-2 Placing O-ring ............iii Precautions ..............iii Preface ........... 4-2 Proper Loading Thermo Scientific 75003621 BIOShield...
- Page 47 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/rotors © 2009-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.
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