Thermo Scientific 7452 Operating And Maintenance Manual page 13

Section 1
Introduction
Pre-set Profiles
(continued)
1-6 7007452 CryoMed Freezer
Pre-Set Profile #6 - Commonly used for hemopoietic stem cells that are
65-100ml in size, resulting in a 0.5°C rate from nucleation to -20°C and
a 1°C per minute cooling rate to a -80°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
S = Sample Temperature
C = Chamber Temperature
C/m = degrees Centigrade per Minute
at 4.0C
to -4.0C
to -45C
to -10.0C
to -20C
to -80C
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