Collecting A Background Spectrum; Measuring A Sample - Agilent Technologies 4500 Series Operation Manual

Analyzing Samples
Do not break the spectrometer seal and attempt to clean interior surfaces.
CAUTION
Breaking the seal will void the warranty.

Collecting a background spectrum

To ensure accuracy of the measurement, it is recommended that the
system is configured in the software to collect a background
spectrum before every sample is analyzed. This provides a baseline
profile of the system conditions with no sample loaded on the
instrument. By collecting a background automatically before each
sample measurement, negative effects of changes in the environment
can be avoided.
For detailed software instructions for collecting a background, refer
to the MicroLab Software Operation Manual.
To ensure an accurate background spectrum is collected, perform a visual
CAUTION
inspection of the ZnSe window surfaces looking for any haze or film present on
the windows from measuring the previous sample. If a film is observed, repeat
the cleaning procedures above until the window surfaces are clear of any
residue.

Measuring a sample

To apply a lubrication sample to the system:
1
2
3
22
Open the sampling device (TumblIR) by rotating the arm
counterclockwise (see Figure 3).
The bottom window should be visible at this point. Place a small
amount of material on the sample window located on the
TumblIR base plate. The bottom window is the 2 mm diameter
yellow material held in place by the surrounding metal disk.
Ensure that the sample covers the entire surface area of the
bottom window
(see
Figure 5
).
Agilent 4500 Series FTIR Operation Manual