Centrifugation - Thermo Scientific Sorvall ST 8 Instruction Manual

Centrifugation

Maximum Loading
The rotor can run at high speeds. The rotor design has sufficient reserve stability even when spinning
at top speed.
The safety system of the centrifuge requires that you do not overload the rotor.
There are two options available for centrifuging samples whose weight, including adapter, exceeds the
maximum permissible load:
• Reduce the fill level.
• Reduce the speed.
Use the table or the formula:
n adm =
n
adm
n
max
Once the rotor has been properly installed, the main switch turned on and the lid closed, you may start
centrifuging.
Starting the Centrifuge Run
Press the START key on the control panel. The centrifuge accelerates to the pre-set speed with the time
display active.
If the speed setting is higher than the maximum permissible speed or RCF-value for the particular
rotor, then the display will show the message „Limit [max. permitted value in rcf or xg]" once the
centrifuge has been started.
Within 10 seconds you can apply this value by pressing the START key again, and the centrifuging
program will continue. Otherwise the centrifuge will stop and you will have to enter a valid number.
You cannot open the lid as long as the centrifuge is running.
Imbalance Indicator
If a load is imbalanced, this will be indicated at speed higher than approx. 300 rpm by the message
"Imbalanced load".
The run will terminate.
Thermo Scientific
WARNING Injuries with fatal consequences can occur when using substanxe mixtures
with a higher density at maximum speed than 1.2
n max Maximum permissible load
--------------------------------------------------------------------- -
Effective load
= admissible speed
= maximum speed
g
.
---------- -
3
cm
Sorvall ST 8 Centrifuge
4
Operation
Centrifugation
35