Agilent Technologies 8453 Operator's Manual page 24

Installing and Operating Your Multicell Transport
Operating Your Multicell Transport
Helpful Hints
When mixing two solutions or adding the stirring bars, bubbles may form
inside the cell. Tap the outside of the cell lightly to free the trapped bubbles.
If your flow cells or cuvettes are dirty and/or you observe that air bubbles
stick on the window surfaces you may use the cuvette cleaning procedure,
described in "Cleaning Cuvettes" on page 35.
Use caps on the cuvettes whenever possible.
Best accuracy can be achieved by not removing the cell from the cell holder
when measuring a blank or between samples. When you must remove cells
make sure that you reinsert them facing the same way as before.
Applications with cycle times greater than five minutes and that require the
highest precision and accuracy should incorporate a blank prior to each new
cycle to compensate for possible fluctuations of the baseline.
Cells with apertures of 2 mm or less are not recommended to be used with
the transport because—depending on the aperture design—the transmitted
light intensity may be decreased.
To compensate for absorbance difference between cuvettes in the multicell
transport cell holder, use the Zero Cell feature in your Agilent ChemStation
software.
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