Agilent Technologies 8453 Operator's Manual page 21

Uv-visible spectrophotometer
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Figure 7
Table 2
Installing and Operating Your Multicell Transport
Installing the Filter Kit
Transmission Characteristics of Filters
Roll-off
265
295
Table 2 shows the type of filters available.
Filter Types and Labels
Label
Filter
Roll-off
UV roll-off
265
265 nm UV cut-off (at 50 %T)
295
295 nm UV cut-off (at 50 %T)
The choice of when to use a filter and which filter to use should be made only
after considering all the factors that affect your measurements. If you decide
that a filter is needed, you should choose a filter that transmits the
wavelengths needed for analytical information and that blocks the
wavelengths that contribute to sample degradation. Often, your final choice
will be a compromise since sometimes even the light at the wavelengths of
analytical interest can affect your sample.
If you do not need a filter for a group of measurements, you can unscrew the
filter from the multicell transport. This permits full light transmission. When
you will not be using the filter rings for an extended period of time you
should store it in a clean plastic bag.
The optical filters of the filter rings must be kept clean. Dirty filters interfere
with light transmission and lower the spectrophotometer performance.
These filters may be cleaned with isopropanol and photographic lens tissues.
21

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