Olympus FLUOVIEW FV1000 User Manual page 37

Sample: single stain of green fluorescence dye (GFP)
3
Unmixing image between GFP and Auto fluorescence
31
Image Analysis (
Image Analysis (
I. When each fluorescence dye point is clear
I. When each fluorescence dye point is clear
and auto fluorescence from cell
1
ROI 2
2
ROI 1
4
5
6
Only GFP image
Unmixing
Unmixing
1. Open the XYL image (GFP +
auto fluorescence).
2. Enclose a point dyed with GFP only
and a point dyed with
auto fluorescence only.
3. From Processing on the menu bar,
select Spectral Deconvolution.
4. Double-click on ROI1(GFP) and
ROI2(Auto fluorescence).
5. Check that the Processing Type is
set to "Normal" and click on Execute .
6. An unmixing image is obtained.
Green color is GFP.
Gray color is Auto fluorescence.
)
)
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