Olympus FLUOVIEW FV1000 User Manual page 129

III. When only the number of types of fluorescence dyes is known
III. When only the number of types of fluorescence dyes is known
From an XYL image where fluorescence dyes with similar fluorescence
spectrums are present together, obtain an unmixed image based on only the
number of types of fluorescence dyes.
Sample: Sample with two unknown types of fluorescence dyes
2
Unmixed image
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Image Analysis (
Image Analysis (
1
3
4
5
Unmixing
Unmixing
(Blind Unmixing
(Blind Unmixing
1. Open an XYL image file for a sample
that has two unknown types of
fluorescence dyes.
2. From Processing on the menu bar,
select Spectral Deconvolution.
3. Click on two Calculate check boxes.
(Click on three boxes when three
types of fluorescence dyes are used.)
4. Check that Processing Type is set to
"Blind" and click on Execute.
5. An unmixed image is obtained.
)
)
)
)
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