Olympus FLUOVIEW FV1000 User Manual page 126

I. When each fluorescence dye point is clear
I. When each fluorescence dye point is clear
From an XYL image where fluorescence dyes with similar fluorescence
spectrums are present together, derive the fluorescence spectrum for each
fluorescence dye and obtain an unmixed image based on the fluorescence
spectrums.
Sample: Double stain of green fluorescence dye (Alexa Fluor 488)
3
5
Unmixed image
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Image Analysis (
Image Analysis (
and green fluorescence dye (YOYO-1)
2
4
6
Unmixing
Unmixing
1. Open an XYL image file with both
Alexa Fluor 488 and YOYO1 applied.
2. Enclose a point dyed with Alexa Fluor
488 only and a point dyed with
YOYO1 only.
3. From Processing on the menu bar,
select Spectral Deconvolution.
4. Double-click on ROI1 and ROI2.
5. Check that the Processing Type is
set to "Normal" and click on Execute.
6. An unmixed image is obtained.
Indicates channel
assignments of
unmixed images
)
)
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