Olympus FLUOVIEW FV1000 User Manual page 157

Laser scanning biological microscope
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Complement of adjusting the image
Complement of adjusting the image
6
5
13
PanX,Y and Rotation reset button
7
8
9
5. Pan X,Y
Be able to move the field of view to
set Pan X,Y without stage action
6. Rotation
Be able to rotate the whole image.
7.
Click "Auto" button to acquire
Optimized Conforcal aperture
Conforcal aperture
conforcal aperture to larger diameter
for dim fluorescence image then, be
able to get the more bright image. But
Z axis resolution gets worse.
8. Laser Intensity
intensity is increase , more bright
image is .
* More increase laser intensity is , more
discoloration image is .
9.
Kalman accumulation
acquisition is repeated to the
specified number of times to provide
an averaged image. Consequently,
noise is averaged and roughness on
the whole image is reduced.
Advantage: The speed of each scan is fast.
Disadvantage: Some blur occurs due to
averaging of images.
change
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More Laser
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Image
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13

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