Olympus FLUOVIEW FV1000 User Manual page 127

I. When each fluorescence dye point is clear
I. When each fluorescence dye point is clear
Sample: single stain of green fluorescence dye (GFP)
1
3
Unmixing image between GFP and Auto fluorescence
29
Image Analysis (
Image Analysis (
and auto fluorescence from cell
ROI 2
2
ROI 1
4
5
6
Only GFP image
Unmixing
Unmixing
1. Open the XYL image (GFP +
auto fluorescence).
2. Enclose a point dyed with GFP only
and a point dyed with
auto fluorescence only.
3. From Processing on the menu bar,
select Spectral Deconvolution.
4. Double-click on ROI1(GFP) and
ROI2(Auto fluorescence).
5. Check that the Processing Type is
set to "Normal" and click on Execute.
6. An unmixing image is obtained.
Green color is GFP.
Gray color is Auto fluorescence.
)
)
29