Olympus FLUOVIEW FV1000 User Manual page 114

Acquisition of a single image (XY plane) (fluorescence image only)
Sample: Double stain of green fluorescence dye (Alexa 488)
Sequential scan (Line Sequential is introduced here.)
4
3
2
5. Right-click on the Image
16
Image Acquisition
Image Acquisition
(Double Stain on XY Image)
(Double Stain on XY Image)
and red fluorescence dye (Alexa 546)
1. Click on the DyeList button. On the
DyeList panel, double-click on a
fluorescence reagent to be used for
observation.
2. Check Sequential and select Line.
3. Adjust the green (Alexa Fluor 488)
image and the red (Alexa Fluor 546)
image.
4. Press the XY button to acquire an
image.
5. Saving the image: Right-click on
the Image Icon displayed on the
Data Manager and select Save
As to save the image.
(Save as Type "oib" or "oif" file
format specifically for the FV10-
ASW software.) The image is
acquired.
Memo
File formats specifically for the FV10-ASW
OIF format:
Creates "a folder that contains an image (16-
bit TIFF)" and "an accessory file," which
cannot be opened separately from each other.
OIB format:
Creates the OIF format files in a single file,
which is convenient for migration and other
operations.
16