Olympus FLUOVIEW FV1000 User Manual page 123

Image Acquisition (Spectral Image on XYL Image)
Image Acquisition (Spectral Image on XYL Image)
Sample: Double stain of green fluorescence dye (Alexa Fluor 488)
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Acquisition of a spectral image (XYL)
and green fluorescence dye (YOYO-1)
Select 488 for the
excitation laser.
Select BS20/80 or
DM405/488.
1. Click on the FV10-ASW software
button
lamp shutter.
Alternatively, click on the
to close the halogen bulb shutter.
2. Click on the
optical path diagram.
3. Make settings as shown below.
3
to close the fluorescence
button to view the
Select Mirror.
Select CHS1 only.
button
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