GE MicroCal iTC200 Operating Instructions Manual page 57

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If creating customized calibration parameters, users must be aware of the DP range
limits when setting reference power and pulse sizes. The reference power must be high
enough to allow all pulses without hitting saturation, and if a pulse size is too small, it
can show abnormally high error.
After the final equilibration phase has completed, the initial delay will begin and the
pulses will be applied as entered. As each pulse completes, Origin will analyze the pulse
region and determine the deflection of the baseline as well as the energy (area) of the
pulse. The requested power and energy will also be displayed as will a percent error for
both power and energy. The reported error in deflection or energy should be less than
1%. If the error is reported as higher than 1%, please contact GE Healthcare.
For a more rigorous analysis, once the calibration is done and the system is
thermostatting again, open the ITC Calibrations project. Click on the Y-Axis Calibration
(DP, uCal/sec) button. Origin will ask for the DP check file. Select the data file just created
and click Open. The computer will think for a few moments. If any of the pulses are out
of specifications, a pop-up will inform you. It will ask you to save.
Origin will show four graphs. The upper left graph holds the raw data. The lower two
graphs show the energy and power of each pulse. The upper right graph displays the
percent error for the energy and power of each pulse. Right-click at the upper right
portion of the graph and select Go To Window from the menu. Check the sizes of the
errors. If any of the errors is greater than 1%, please contact GE Healthcare.
MicroCal iTC Operating Instructions 28-9639-77 AA
200
Select MicroCal iTC
200
Check. Once the menu has been selected, the Calibration Pulse Setup window will
appear. This window allows the calibration pulses to be modified.
Enter individual pulse parameters by first selecting a pulse or multiple pulses, then
enter the desired parameter value into the pulse parameter boxes (Calibration
Power, Pulse Duration and Pulse spacing). Users are encouraged to simply use the
default y-axis calibration parameters.
After the run and pulse parameters are entered, click on the Start Run button to
start the run. The ITC will equilibrate in the same manner as it would during a
titration experiment.
software menu ITC => Start ITC Calibration Run => Y Axis
Maintenance 5
Y-axis calibration check 5.8
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