Pipette Calibration; Y-Axis Calibration Check - GE MicroCal iTC200 Operating Instructions Manual

5 Maintenance
5.7

Pipette calibration

5.7 Pipette calibration
The pipette may need to be re-calibrated, especially after a new pipette is installed. The
"ITC => Pipette Tools" menu option will open the following popup.
Figure 5-7. Pipette menu.
The Initialize Pipette section will make the software run a routine that checks the
sensors on the pipette and makes sure that the software knows certain constants of the
pipette. The checkbox at the bottom of the section will cause the software to run this
check every time it starts.
The Open Port Calibration section contains the controls for adjusting the position of the
plunger when the fill port is open. Use the distance slider bar and the up and down
buttons to move the plunger tip to just above the fill port in the upper side of the glass
syringe bore. Once the position is correct, click the Calibrate Open Fill Port button to
have the software set the current location as the open fill port location. Click the Open
and Close buttons in the bottom section to check the calibration.
5.8

Y-axis calibration check

It is recommended that the y-axis calibration be checked every few months to ensure
accurate data acquisition. The automatic calibration check routine will send a series of
pulses to the cell heaters, dissipating a known power. The offset in the DP as a result of
this power is analyzed in comparison to the correct DP offset.
Make sure the cells are clean, and fill both cells with degassed, distilled water. Load the
titration syringe with water and insert it into the sample cell. It is recommended that
Origin for real-time data be enabled.
To begin the y-axis calibration check procedure:
54
MicroCal iTC Operating Instructions 28-9639-77 AA
200