Routine Editor: Waveform Editor - Sutter Instrument IPA Operation Manual

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The default setting is '4', which when paired with the default Leak Ratio, is
equivalent to commonly used P/4 settings.
Note that as each leak pulse replicates the stimulus waveform, larger
numbers of leak pulses can greatly increase the total duration of a sweep
duriing acquisition.
Leak Ratio
Set the leak subtraction pulse size relative to the main waveform pulse,
using a ratio between +1 and -1. The setting should be low enough that no
eletrically-gated ion channels are activated. For instance, a Leak Ratio
setting of 0.25 will generate leak pulses at ¼ the amplitude of the main
stimulus waveform, a Leak Ratio of 0.2 will generate leak pulses at 1/5 the
main pulse.
Note that the program scales the leak subtraction pulses based upon the
Leak Ratio setting, not the number of Leak Pulses. This means that the
Leak Ratio can be set independently from Num Leak Pulses if desired,
instead of being coupled together.
Tip: As an alternate way to avoid electrically activating ion channels, use a
negative ratio to reverse the polarity of the leak pulses relative to the main
pulse.
Leak Holding
Set the leak pulses holding level. This can be set differently from the
routine's main holding level, for flexibility in finding a suitable voltage range
for the leak pulses.

4.15 Routine Editor: Waveform Editor

INTEGRATED PATCH AMPLIFIER OPERATION - M – R . 1.0.6 (20160729)
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