Thermo Scientific 370 Operating And Maintenance Manual
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Thermo Scientific 370 Operating And Maintenance Manual

370 series steri-cycle co2 incubator direct heat with sterilization cycle
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Model 370 Series
Steri-Cycle CO
Incubator
2
Direct Heat with Sterilization Cycle
Operating and Maintenance Manual
7000370 Rev. 12

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Summary of Contents for Thermo Scientific 370

  • Page 1 Model 370 Series Steri-Cycle CO Incubator Direct Heat with Sterilization Cycle Operating and Maintenance Manual 7000370 Rev. 12...
  • Page 2 Factory default for gas guard to ON from OFF 21574/IN-3124 8/12/03 Updated temp sensor from 290137 to 290184 (glass encapsulated) 21237/IN-3068 2/24/03 Upgraded inner door components (370-200-9) 20959/IN-3048 10/1/02 Added spacers to power supply mounting 20374/IN-2994 11/12/01 Updated 200-6 drawing - Kaizen, board hardware 20460 Corrected 4-20mA board temp output to 12.5 - 162.5°C...
  • Page 3 Preface Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲ Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲...
  • Page 4 Preface Important operating and/or maintenance instructions. Read the accompanying text carefully. Potential electrical hazards. Only qualified persons should perform procedures associated with this symbol. Equipment being maintained or serviced must be turned off and locked off to prevent possible injury. Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be damaged by elevated temperatures.
  • Page 5 Preface Do You Need Information or Assistance on Thermo Fisher Scientific Products? If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at: 1-740-373-4763 Direct 1-888-213-1790 Toll Free, U.S. and Canada 1-740-373-4189 http://www.thermo.com/forma Internet Worldwide Web Home Page services.controlenv@thermo.com Service E-Mail Address Sales Support...
  • Page 6 Preface Warranty Notes Information You Should Know Before Requesting Warranty Service • Locate the model and serial numbers. A serial tag is located on the unit itself. • For equipment service or maintenance, or with technical or special application inquiries, contact Technical Services at 1-888-213-1790 or 1-740-373-4763 (USA and Canada).

  • Page 8: Table Of Contents

    Table of Contents Section 1 Installation and Start-Up ........1-1 Control Panel Components .
  • Page 9 Table of Contents Section 3 Configuration ..........3-1 Turn Audible Alarm ON/OFF .
  • Page 10 Table of Contents Section 6 Factory Options ..........6-1 Remote Alarms .

  • Page 12: Installation And Start-Up

    Installation and Start-Up Section 1 Figure 1-1. Steri-Cycle Components • Outer Door - Reversible to opposite swing, see Section 5 • Inner Door - Reversible to opposite swing, see Section 5 • Chamber Gas Sample Port - Used for sampling chamber CO content using a FYRITE or similar instrument.

  • Page 13: Control Panel Components

    Section 1 Installation and Start-Up Control Panel Silence - Mutes the audible alarm. Components Alarm Indicator - Light pulses on/off during an alarm condition. Mode Select Switch - Used to select Run, Setpoints, Calibration and System Configuration Modes. Message Center - Displays system status. Mode Select Indicators - Run: Run Menu Set: Set Points Menu...

  • Page 14: Keypad Operation

    Section 1 Installation and Start-Up Keypad Operation The Model 370 Series incubator has four basic modes which allow incubator setup: Run, Setpoints, Calibration and System Configuration. • Run is the default mode in which the incubator will normally be during operation.

  • Page 15: Displays

    Section 1 Installation and Start-Up Keypad Operation Scroll for Parameters Arrows: Steps the operator through parameters of SET, CAL and CONFIG Modes. The right arrow goes to the next (continued) parameter, the left arrow returns to the previous parameter. Up Arrow: Increases or toggles the parameter value selected in SET, CAL, and CONFIG Modes.

  • Page 16: Stacking The Incubators

    Section 1 Installation and Start-Up Stacking the Warning Install stacked units against a wall or similar structure. ▲ Incubators Warning With incubators in a stacked configuration, do not leave both exterior doors open at the same time. ▲ Warning If the units have been in operation, turn them both off and disconnect the power before beginning any service work.
  • Page 17 Section 1 Installation and Start-Up 5. Insert the stacking brackets into the slots at the back of the stacked units as shown in Figure 1-7. 6. Align the slotted holes in the brackets with the mounting holes on the back of the top incubator.

  • Page 18: Preliminary Cleaning

    Section 1 Installation and Start-Up Preliminary Cleaning 1. Remove vinyl from shelf channels, duct sheets, and air duct, if present. 2. Using a suitable laboratory disinfectant, clean all interior surfaces. Installing the Shelves 1. Install the large sheet metal side ducts with the tabs facing into the center of the chamber with their slots up.

  • Page 19: Access Port Filter & Co Sensor Cover Plate

    Section 1 Installation and Start-Up Access Port Filter & 1. Locate the opening in the top left corner on the inside of the chamber. Remove the tape covering the opening on the outside of the unit. Sensor Cover Plate 2. Locate the stopper with filter in the hardware bag. Lift the metal port cover and install the assembly in the opening inside the chamber.

  • Page 20: Leveling The Unit

    Section 1 Installation and Start-Up Installing the HEPA 1. Remove the filter from the shipping box. Filter (continued) 2. Remove the plastic coating from the filter, using caution not to touch the filter media. 3. Install the filter as shown in Figure 1-13. Refer to Section 5 for HEPA filter maintenance.

  • Page 21: Filling The Humidity Pan

    Section 1 Installation and Start-Up Plug the provided power cord into the power inlet connector on the back of the cabinet (Figure 1-14), then into grounded, dedicated, electrical circuit. Figure 1-14. Back of Cabinet Filling the Humidity Fill the humidity pan with sterile distilled water to within 1/2” of the top. Place the pan directly in the center of the incubator floor.

  • Page 22: Connecting The Co Gas Supply

    Section 1 Installation and Start-Up Even high purity water can contain bacteria and organic contaminants. Water should always be sterilized or treated with a decontaminant, safe for use with stainless steel as well as safe for the product, prior to being introduced into the humidity pan.

  • Page 23: Incubator Start-Up

    See Chart 1-1 for more detail. Setting the Operating All Model 370/380 Series incubators have an operating temperature range of 10°C to 50°C, depending on ambient temperature. The incubator is Temperature shipped from the factory with a temperature setpoint of 10°C.

  • Page 24: Setting The Operating Temperature

    ▲ Model 370/380 Series incubators are equipped with a secondary temperature monitoring system to monitor the air temperature inside the cabinet. This system is designed as a safety device to turn off all heaters in the event of a temperature control failure.

  • Page 25: Setting The Overtemp Setpoint

    Section 1 Installation and Start-Up Setting the Overtemp To set the Overtemp setpoint: Setpoint (continued) 1. Press the Mode key until the Set indicator lights 2. Press the right arrow until Otemp XX.X is displayed in the message center 3. Press the up or down arrow key until the desired Overtemp setpoint is displayed 4.
  • Page 26 Section 1 Installation and Start-Up Thermo Fisher Scientific Steri-Cycle CO Incubator 1-15...

  • Page 28: Calibration

    Calibration Section 2 After the unit has stabilized, several different systems can be calibrated. In the calibration mode, the air temperature, CO2 and RH levels can be calibrated to reference instruments. To access the calibration mode, press the Mode key until the Cal indicator lights. Press the right and/or left arrow until the appropriate parameter appears in the message center.

  • Page 29: Thermal Conductivity Co System

    5. Press the Mode key to return to Run or the right/left arrow to go to next/previous parameter. Thermal Conductivity Models 370 and 371 have a thermal conductivity (T/C) CO sensor. Thermal conductivity of the incubator atmosphere is not only effected by...
  • Page 30 Section 2 Calibration Thermal Conductivity 1. Make sure the stabilization periods outlined above are followed. System (continued) 2. Sample the chamber atmosphere through the sample port with an independent instrument. Sample the atmosphere at least 3 times to ensure accuracy of the instrument. 3.

  • Page 31: Infrared Co System

    6. Press the Mode key to return to Run mode. Relative Humidity All Model 370/380 Series incubators can be equipped with an optional direct-readout relative humidity sensor. This is a readout only of the chamber relative humidity level. It does not provide any control of the relative humidity in the cabinet.

  • Page 32: Relative Humidity

    Section 2 Calibration Relative Humidity If a reliable RH measuring device is not available, the display can be calibrated to a typical level; (continued) 1. Follow the RH stabilization periods outlined above. 2. With a full humidity pan and stable temperature, the relative humidity in the chamber will be 95%.
  • Page 33 Section 2 Calibration Steri-Cycle CO Incubator Thermo Fisher Scientific...

  • Page 34: Configuration

    Configuration Section 3 Several features available in the Configuration Mode allow custom setup of the incubator. These features are listed with descriptions below. All features may not be necessary in all applications, but are available if needed. To enter Configuration mode, press the Mode key until the Config indicator lights.

  • Page 35: Set Replace Hepa Filter Reminder

    Section 3 Configuration Set REPLACE HEPA A HEPA filter replacement timer can be set for a specific amount of time, from 1 to 12 months of actual unit running time. Time will not accrue filter reminder when the unit is turned off. The default time is 6 months. When the allotted time runs out, REPLACE HEPA appears in the display and the visual alarm flashes.

  • Page 36: Set Low Temp Alarm Limit (Tracking Alarm)

    Section 3 Configuration Set Low Temp Alarm The low temp alarm limit is the deviation from the temperature setpoint which causes a low temp alarm. The low temp alarm is variable from 0.5° Limit (tracking alarm) below setpoint to 5° below setpoint. The factory setting is 1° below setpoint.

  • Page 37: Set Low Co 2 Alarm Limit (Tracking Alarm)

    Section 3 Configuration Set Low CO Alarm The low CO alarm limit is the deviation from the CO setpoint which will cause a low CO alarm. The setpoint is variable from 0.5% CO Limit (tracking alarm) below setpoint to 5.0% CO below setpoint.

  • Page 38: Enable Co Alarms To Trip Contacts

    Section 3 Configuration Enable CO Alarms to High and Low CO alarms can be programmed to trip the remote alarm contacts. A setting of ON allows this, a setting of OFF blocks CO alarms Trip Contacts from tripping the contacts. The factory setting is ON. 1.

  • Page 39: Set New Span Number For T/C Co Sensors

    Section 3 Configuration Set New Span Number If a new T/C CO sensor is being installed, the two numbers on the factory installed sticker on the T/C cell must be entered to calibrate the for T/C CO Sensors in the unit. Note For the technician’s convenience, a label containing the two numbers on the T/C cell is affixed inside the electronics drawer.

  • Page 40: Enable Rh Alarms To Trip Contacts

    Section 3 Configuration Enable RH Alarms to The low RH alarm can be programmed to trip the remote alarm contacts. A setting of ON allows this, a setting of OFF blocks the RH alarm from Trip Contacts tripping the contacts. The factory setting is ON. 1.

  • Page 41: Select A Primary Tank W/ Gas Guard Option

    Section 3 Configuration Select a Primary Tank On units equipped with the Gas Guard option, a primary tank can be selected. The primary tank will be either Tank 1 or 2. The factory setting w/ Gas Guard Option is Tank1. 1.

  • Page 42: Set An Rs485 Communications Address

    Section 3 Configuration Set an RS485 On units with the RS485 option, direct communication with the Model 1535 alarm system can be established. Each piece of equipment connected Communications Address to the 1535 must have a unique address. An address of 0-24 can be entered for the incubator.
  • Page 43 Section 3 Configuration 3-10 Steri-Cycle CO Incubator Thermo Fisher Scientific...
  • Page 44 Section 3 Configuration Thermo Fisher Scientific Steri-Cycle CO Incubator 3-11...
  • Page 45 Section 3 Configuration 3-12 Steri-Cycle CO Incubator Thermo Fisher Scientific...

  • Page 46: Alarms

    Alarms Section 4 The Model 370/380 Series incubator alarm system is shown in the table below. When an alarm is active, the message appears in the LED message center. Pressing Silence disables the audible alarm for the ringback period. However, the visual alarm continues until the incubator returns to a normal condition.

  • Page 47: Temp Controller Failure Tmp Cntr Err

    Section 4 Alarms Temp Controller Failure In addition to other safety features designed into Model 370 Series incubators, a thermostat is also provided to monitor the cabinet’s TMP CNTR ERR temperature. In the unlikely event of a temperature control failure, the thermostat will turn off all heaters at a cabinet temperature of 160°C,...

  • Page 48: Preventive Maintenance

    Do not use powdered gloves for tissue cultures. 401 Millcreek Road, Box 649 • Marietta, Ohio 45750 USA • 740-373-4763 http://www.services.controlenv@thermo.com USA and Canada 888-213-1790 • Telefax: 740-373-4189 • Preventive Maintenance for Model 370/380 Series Incubators Refer to Manual Action Daily...

  • Page 50: Routine Maintenance

    Routine Maintenance Section 5 Warning If the unit has been in service, turn it off and disconnect the power cord connector before proceeding with any maintenance. ▲ Caution Before using any cleaning method except those recommended by the manufacturer, users must check with the manufacturer that the proposed method will not damage the equipment.

  • Page 51: Clean Cabinet Exterior

    Section 5 Routine Maintenance 1. Remove the shelves, access port filter assembly, HEPA filter, air sample filter and tubing, and the left and right duct sheets. Discard the HEPA filters and the access port filter assembly. See Figure 5-1. Figure 5-1. Component Locations 2.

  • Page 52: Clean Glass Doors

    Section 5 Routine Maintenance Clean Glass Doors The chamber glass door and the optional independent inner glass doors may be cleaned using the same disinfectant as used on the incubator interior. It is imperative that they be rinsed with sterile distilled water to remove the disinfectant residue.
  • Page 53 Section 5 Routine Maintenance Reverse Door Swing 1. Locate the small cover plate securing the door cord (Figure 5-3). Remove the (continued) screws from the plate and disconnect the door cord from the connector behind the plate. Figure 5-3. Cover Connector 2.

  • Page 54: Hepa Filter Maintenance

    Section 5 Routine Maintenance 11. Install the door handle opposite the hinges. Install screw covers. 12. To secure the outer door to the unit, the Phillips screws on the side of the unit must be removed and installed on the opposite side. 13.

  • Page 55: Replace Air Sample Filter

    Section 5 Routine Maintenance Replace Air Sample 1. Connect one end of the air sample filter to the hose barb on the chamber ceiling. Filter 2. Connect the other end of the filter to the metal tubing on the HEPA filter adapter (Figure-5-8).

  • Page 56: Major Components

    Section 5 Routine Maintenance Major Components Refer to Figure 5-9. Some components shown are factory installed options. Figure 5-9. Electronics Drawer Thermo Fisher Scientific Steri-Cycle CO Incubator...

  • Page 57: Sterilization Cycle

    Section 5 Routine Maintenance Sterilization Cycle Information You Need to Know Before Starting a Cycle • The Sterilization Cycle requires approximately 12 hours - heat-up (2-4 hours), sterilize (2 hours), and cool down (6-8 hours). Additional time is needed to verify the calibration of temperature and CO after the cycle is complete.
  • Page 58 Section 5 Routine Maintenance Checkpoints What if? Then Cycle does not initiate or terminates in mid-cycle Check for alarms: SYS IN OTEMP, AIR SNSR ERR, TMP CTRL ERR Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit.. Do not use second unit during Sterilization Cycle.
  • Page 59 Section 5 Routine Maintenance Sterilization Cycle Warning The Sterilization Cycle heats the incubator interior surfaces to 140°C. Contact with any surface inside the outer door during this cycle may result in burns. ▲ 1. Empty the water from the humidity pan and place the pan back into the incubator.
  • Page 60 Section 5 Routine Maintenance Note To remove the IR sensor, if applicable, first power the unit off. Then turn the two thumbscrews on the top duct until the cover plate disengages. Secured on the inside of the plate is a dummy sensor (Figure 5-12). ▲...
  • Page 61 Section 5 Routine Maintenance 6. After ENTER is pressed, the Heat Phase initiates. The green LED flashes and the display toggles between “STERILIZING” and “HEAT PHASE”. During this period, the HEAT light will be on and the cabinet heats to sterilization temperature. Figure 5-15.
  • Page 62 Section 5 Routine Maintenance 9. Completed Sterilization Cycle with T/C CO sensor - When the temperature cools down to the original set operating temperature or 30°C, whichever is higher, the display toggles between “CYC COMPLETE”, “REPL HEPAs”, and “PRESS ENTER”. The green LED is lighted but no longer flashes.
  • Page 63 Section 5 Routine Maintenance 10. Open the chamber and install a new HEPA filter, air sample filter and access port filter assembly. Press ENTER. 11. When ENTER is pressed, the LED goes out, the display returns to “SYSTEM OK”, and the HEPA filter replacement timer is reset. 12.

  • Page 64: Factory Options

    Factory Options Section 6 A description of the factory option connections to external equipment follows. Remote Alarms A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet. Refer to Figure 6-5 for the location of the alarm connector.

  • Page 65: Rs485 Interface

    Section 6 Factory Options RS485 Interface All incubator models can be purchased with the RS485 communications option. This option allows the incubator to be directly connected to a (P/N 1900085) Model 1535 alarm system without the use of a communications module. A junction box is provided with each RS485 option.
  • Page 66 Section 6 Factory Options Analog Output Boards For the 0-1V and 0-5V boards, the recording device must supply a load >/=1000 ohm. For the 4-20mA board, the recorder must supply a load of (continued) </=100 ohm. To wire in the analog output board, use a shielded 22 gauge, 3-conductor wire (P/N 73041), maximum length 50 ft (15.2m).

  • Page 67: Co 2 Gas Guard

    Section 1 of this manual. ▲ (P/N 1900086) Model 370 Series incubators can be equipped with a built-in Gas Guard system that will operate with a CO gas supply. The Gas Guard uses two pressure switches to continuously monitor the pressures of two...

  • Page 68: Connecting Co

    Section 6 Factory Options Connecting CO The CO inlets for the Gas Guard are located on the rear of the cabinet. Using 1/4” ID tubing, connect one of the CO supply tanks to the fitting Supplies labeled CO2 Inlet #1 Tank. Connect the second CO supply tank to the fitting labeled CO2 Inlet #2 Tank.

  • Page 69: Humidity Readout

    Gas Guard continues to detect that both gas supply pressures are below 10 psig (0.690 bars). Humidity Readout Model 370/380 Series incubators can be equipped with a humidity sensor to monitor the relative humidity inside the chamber. The sensor is located (P/N 1900091) on the chamber ceiling and provides a signal that is displayed in 1% increments on the control panel.

  • Page 70: Specifications

    Specifications Section 7 *Specifications are based on nominal voltages of 115V or 230V in ambients of 22°C to 25°C. Temperature Control ..±0.1°C Microprocessor PID Control Setpoint ..Digital - Touch pad, 0.1°C Range .

  • Page 71: Section 7 Specifications

    Section 7 Specifications Shelves Dimensions ..18.5” x 18.5” (47cm x 47cm) Construction ..Stainless steel (belt sanded, both sides) Surface area ..2.4 sq. ft. (0.22 sq. m) per shelf Max.
  • Page 72 Section 7 Specifications Copper Options Perforated shelf Customer Installed: P/N 224166 - solid copper Humidity pan Customer Installed: P/N 237015 - solid copper Copper interior components kit Factory installed: Replace stainless steel duct work, shelves, and humidity pan with solid copper P/N 1900095 Optional Data Outputs Analog .
  • Page 74 Spare Parts Section 8 Part Number ..Description 190699 ...Removable feather gasket 190670 ...Duct sheet and shelf channel kit 191650 .
  • Page 78 BACK PANEL STUD DETAIL BACK PANEL STUD DETAIL 370-CO2-INLET-2 370-CO2-INLET-2 72015 3/16" VINYL TUBING 6" 72015 3/16" VINYL TUBING 6" 370_CO2_INLET_1 950013 VINYL INLET TUBE 370 , 8" 950013 VINYL INLET TUBE 370 , 8" 840020_FITTING 840020_FITTING .250 BULKHEAD FITTING .250 BULKHEAD FITTING...
  • Page 79 360236_SCSWITCH GREEN PUSHBUTTON SWITCH GREEN PUSHBUTTON SWITCH THIS DOCUMENT CONTAINS PROPRIETARY THIS DOCUMENT CONTAINS PROPRIETARY MODEL/PART NAME: 370 SERIES INCUBATOR 370 SERIES INCUBATOR INFORMATION AND SUCH INFORMATION IS NOT TO INFORMATION AND SUCH INFORMATION IS NOT TO DWG TITLE: CONTROL PANEL BEZEL ASSEMBLY...
  • Page 84 Thermo Fisher Scientific Steri-Cycle CO Incubator...
  • Page 85 Steri-Cycle CO Incubator Thermo Fisher Scientific...
  • Page 86 Thermo Fisher Scientific Steri-Cycle CO Incubator...
  • Page 88 Steri-Cycle CO Incubator Thermo Fisher Scientific...
  • Page 89 Thermo Fisher Scientific Steri-Cycle CO Incubator...
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  • Page 92 Thermo Fisher Scientific Steri-Cycle CO Incubator 10-1...
  • Page 93 Section 10 Section title 10-2 Steri-Cycle CO Incubator Thermo Fisher Scientific...
  • Page 94 Thermo Fisher Scientific Steri-Cycle CO Incubator 11-1...
  • Page 96 Thermo Fisher Scientific Controlled Environment Equipment 401 Millcreek Road Marietta, Ohio 45750 United States www.thermofisher.com...

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