Calibrating Volumes - Qiagen QIAcube ht User Manual

QIAcube HT Software
11. If the (the pipetting head) is accidentally moved too far
12. When the selected "Level at bottom of well" position is
13. Once all plates have been height calibrated, click
5.13.3

Calibrating volumes

A direct relationship exists between the rotation of the motor
shaft (P-axis) and the vertical movement of the pipet piston
(i.e., volume aspirated or dispensed). Volume calibration
defines the number of units that the P-axis motor must move
to accurately deliver a given volume of liquid.
Volume calibration is performed using the "Volume
calibration" wizard. During installation, the preinstalled
settings can be set up using "Option 1" from the "Volume
calibration" wizard.
Before starting the first run on a newly installed QIAcube HT
volume calibration has to be done. If no volume calibration
has been done an error is shown at start of a run.
Opening the "Volume calibration" wizard
1. Start the QIAcube HT Software.
2. From the options that appear, select "Empty Project (last
3. From the "Options" menu in the menu bar, select "Robot
5-80
downwards in the Z direction (i.e., touches the base),
ensure that the pipetting head is returned to the proper
position by clicking "Home Z-axis".
suitable, click "OK" to set the correct height calibration.
"Close" to exit the wizard.
For more details, see Section 5.2, page 5-3.
used layout)" or "Empty Project (default layout).
Setup/Calibrate Volumes".
The "Volume Calibration Wizard" dialog box appears.
QIAcube HT User Manual 07/2013